2023
Bacteria require phase separation for fitness in the mammalian gut
Krypotou E, Townsend G, Gao X, Tachiyama S, Liu J, Pokorzynski N, Goodman A, Groisman E. Bacteria require phase separation for fitness in the mammalian gut. Science 2023, 379: 1149-1156. PMID: 36927025, PMCID: PMC10148683, DOI: 10.1126/science.abn7229.Peer-Reviewed Original ResearchConceptsMammalian gutTranscription termination factor RhoTermination factor RhoGene regulationTranscription terminationMechanisms bacteriaBacteria interactionsHuman commensalValuable targetBacteriaRhoGut microbiotaFitnessNovel clinical applicationsTherapeutic manipulationGutHuman healthCommensalRegulation
2019
A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis
Pokorzynski ND, Brinkworth AJ, Carabeo R. A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis. ELife 2019, 8: e42295. PMID: 30938288, PMCID: PMC6504234, DOI: 10.7554/elife.42295.Peer-Reviewed Original ResearchConceptsSalvage pathwayNovel promoter elementIron-dependent regulationStress response regulatorGlobal stress response regulatorIron-dependent regulatorTermination of transcriptsIntergenic region upstreamHost immune effectorsResponse regulatorTranscriptional regulationObligate intracellular bacteriumIron starvationIntergenic regionPromoter elementsPromoter upstreamTryptophan biosynthesisStress responseRegion upstreamIron-dependent mechanismEssential nutrientsIntracellular bacteriumChlamydia trachomatisImmune effectorsRegulation