2001
Anomalous apical plasma membrane phenotype in CK8-deficient mice indicates a novel role for intermediate filaments in the polarization of simple epithelia
Ameen N, Figueroa Y, Salas P. Anomalous apical plasma membrane phenotype in CK8-deficient mice indicates a novel role for intermediate filaments in the polarization of simple epithelia. Journal Of Cell Science 2001, 114: 563-575. PMID: 11171325, DOI: 10.1242/jcs.114.3.563.Peer-Reviewed Original ResearchConceptsIntermediate filamentsPolarized epithelial cellsApical membrane proteinsEpithelial cellsSyntaxin 3Apical domainFemale sterilityMembrane proteinsApical markerNovel functionGamma-tubulinNovel roleApical poleSimple epitheliaCell typesColorectal hyperplasiaCK intermediate filamentsNull micePhenotypeBasolateral levelsNecrotic cellsMembrane phenotypeCellsFilamentsCytoplasm of enterocytes
2000
Microvillus Inclusion Disease: A Genetic Defect Affecting Apical Membrane Protein Traffic in Intestinal Epithelium
Ameen N, Salas P. Microvillus Inclusion Disease: A Genetic Defect Affecting Apical Membrane Protein Traffic in Intestinal Epithelium. Traffic 2000, 1: 76-83. PMID: 11208062, DOI: 10.1034/j.1600-0854.2000.010111.x.Peer-Reviewed Original ResearchConceptsMicrovillus inclusion diseaseApical membrane trafficMembrane protein trafficVacuolar apical compartmentApical membrane markersMicrovillus inclusionsF-actin layerBasolateral proteinsFirst genetic defectMembrane trafficTissue culture epithelial cellsProtein trafficApical exocytosisLater stepsApical membraneMembrane markersTerminal webImmunofluorescence analysisApical cytoplasmGenetic defectsEpithelial cellsProteinApical compartmentIntestinal epitheliumNormal microtubules
1996
A delta F508 mutation in mouse cystic fibrosis transmembrane conductance regulator results in a temperature-sensitive processing defect in vivo.
French PJ, van Doorninck JH, Peters RH, Verbeek E, Ameen NA, Marino CR, de Jonge HR, Bijman J, Scholte BJ. A delta F508 mutation in mouse cystic fibrosis transmembrane conductance regulator results in a temperature-sensitive processing defect in vivo. Journal Of Clinical Investigation 1996, 98: 1304-1312. PMID: 8823295, PMCID: PMC507556, DOI: 10.1172/jci118917.Peer-Reviewed Original Research