2017
Linaclotide activates guanylate cyclase‐C/cGMP/protein kinase‐II‐dependent trafficking of CFTR in the intestine
Ahsan K, Tchernychev B, Kessler MM, Solinga RM, Arthur D, Linde CI, Silos‐Santiago I, Hannig G, Ameen NA. Linaclotide activates guanylate cyclase‐C/cGMP/protein kinase‐II‐dependent trafficking of CFTR in the intestine. Physiological Reports 2017, 5: e13299. PMID: 28592587, PMCID: PMC5471438, DOI: 10.14814/phy2.13299.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell Line, TumorCell MembraneCyclic AMP-Dependent Protein KinasesCyclic GMPCyclic GMP-Dependent Protein Kinase Type IICystic Fibrosis Transmembrane Conductance RegulatorGuanylyl Cyclase C AgonistsHumansIntestinal MucosaMalePeptidesProtein TransportRatsRats, Sprague-DawleyReceptors, Guanylate Cyclase-CoupledSignal TransductionConceptsRat intestinal loopsLinaclotide treatmentFluid secretionIntestinal loopsCystic fibrosis transmembrane conductance regulatorCell surfaceFibrosis transmembrane conductance regulatorCell surface traffickingChronic idiopathic constipationIrritable bowel syndromeTransmembrane conductance regulatorIntestinal fluid secretionCell surface translocationReceptor guanylyl cyclaseHuman intestinal tissueCaco-2BBe cellsCFTR traffickingCFTR pathwaySubapical compartmentCellular signalingCGMP/PKGProtein kinaseSurface biotinylationIdiopathic constipationPathway components
2016
Identification of intestinal ion transport defects in microvillus inclusion disease
Kravtsov DV, Ahsan MK, Kumari V, van Ijzendoorn SC, Reyes-Mugica M, Kumar A, Gujral T, Dudeja PK, Ameen NA. Identification of intestinal ion transport defects in microvillus inclusion disease. AJP Gastrointestinal And Liver Physiology 2016, 311: g142-g155. PMID: 27229121, PMCID: PMC4967175, DOI: 10.1152/ajpgi.00041.2016.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCaco-2 CellsChloride-Bicarbonate AntiportersCystic Fibrosis Transmembrane Conductance RegulatorEnterocytesGene Expression RegulationHumansIon TransportJejunumMalabsorption SyndromesMembrane Transport ProteinsMicrovilliMucolipidosesMyosin Heavy ChainsMyosin Type VPhenotypePhosphoproteinsRNA InterferenceSignal TransductionSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSulfate TransportersTranscription FactorsTransfectionYAP-Signaling ProteinsConceptsMicrovillus inclusion diseaseStool lossVillus atrophyInclusion diseaseEnterocyte maturationMicrovillus inclusionsIntestinal fluid transportIntestinal cell modelIon transport defectImmunohistochemical stainingSecretory diarrheaBrush border defectsImmature enterocytesC2BBe cellsT84 cellsElectrophysiological approachesCFTR ion transportBB membraneLoss of MYO5BEnterocytesFunctional CFTRDiarrheaAtrophyFunction mutationsCFTR localization