2022
Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID)
Ahsan K, dos Reis DC, Barbieri A, Sumigray KD, Nottoli T, Salas PJ, Ameen NA. Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID). Journal Of Clinical Medicine 2022, 11: 4179. PMID: 35887942, PMCID: PMC9319011, DOI: 10.3390/jcm11144179.Peer-Reviewed Original ResearchMicrovillus inclusion diseaseSevere diarrheaInclusion diseaseFluid secretionCystic fibrosis transmembrane conductance regulatorDouble knockout miceMicrovillus inclusionsGlucocorticoid-inducible kinase 1Carbohydrate malabsorptionKnockout miceIntestinal carbohydrateDiarrheaDiarrheal diseaseMalabsorptionMiceVillus enterocytesDiseaseLoss of MYO5BFunction mutationsApical cystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorKinase 1SecretionIntestineTransmembrane conductance regulator
2013
Role of Myosin1a in regulated exocytosis of CFTR in villus enterocytes
Hoekstra N, Kravtsov D, Mooseker M, Ameen N. Role of Myosin1a in regulated exocytosis of CFTR in villus enterocytes. The FASEB Journal 2013, 27: 913.11-913.11. DOI: 10.1096/fasebj.27.1_supplement.913.11.Peer-Reviewed Original ResearchSurface biotinylationRegulated exocytosisCFTR trafficSurface CFTRSubapical endosomesBrush border membraneMembrane traffickingCFTR deliveryKD cellsKnockdown cellsCFTR channelsCFTR distributionVillus enterocytesMolecular mechanismsEnterocyte brush border membraneCFTRBiotinylationConfocal microscopyEndosomesIntestinal brush border membraneMouse intestineExocytosisCGMP agonistMyo1aBorder membrane
1995
A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator
Ameen N, Ardito T, Kashgarian M, Marino C. A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator. Gastroenterology 1995, 108: 1016-1023. PMID: 7535272, DOI: 10.1016/0016-5085(95)90198-1.Peer-Reviewed Original ResearchConceptsProximal small intestineCrypt epithelial cellsVillus cellsSmall intestineHuman proximal small intestineCystic fibrosis transmembrane conductance regulatorEpithelial cellsSurface marker expressionFibrosis transmembrane conductance regulatorBrush border sucraseTransmembrane conductance regulatorIntestinal villus cellsBACKGROUND/Marker antibodiesMarker expressionUnique subsetVillus enterocytesConductance regulatorIntestineLactase expressionBrush border