Functional vacuolar ATPase (V-ATPase) proton pumps traffic to the enterocyte brush border membrane and require CFTR
Collaco AM, Geibel P, Lee BS, Geibel JP, Ameen NA. Functional vacuolar ATPase (V-ATPase) proton pumps traffic to the enterocyte brush border membrane and require CFTR. American Journal Of Physiology - Cell Physiology 2013, 305: c981-c996. PMID: 23986201, PMCID: PMC4109618, DOI: 10.1152/ajpcell.00067.2013.Peer-Reviewed Original ResearchConceptsV-ATPaseCystic fibrosis transmembrane conductance regulator (CFTR) channelV-ATPase complexV-ATPase functionBrush border membraneProton effluxRat Brunner's glandsIntestinal cellsCAMP/PKACaco-2BBe cellsBorder membraneApical domainCoimmunoprecipitation studiesCFTR distributionVacuolar ATPasesSubapical cytoplasmSpecific subunitsCAMP stimulationProton pumpCAMP treatmentEnterocyte brush border membraneSodium-hydrogen exchangerHydrogen exchangerApical membraneCFTRRegulated traffic of anion transporters in mammalian Brunner's glands: a role for water and fluid transport
Collaco AM, Jakab RL, Hoekstra NE, Mitchell KA, Brooks A, Ameen NA. Regulated traffic of anion transporters in mammalian Brunner's glands: a role for water and fluid transport. AJP Gastrointestinal And Liver Physiology 2013, 305: g258-g275. PMID: 23744739, PMCID: PMC3742856, DOI: 10.1152/ajpgi.00485.2012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnion Transport ProteinsAquaporin 5BicarbonatesBiological TransportBrunner GlandsCarbacholCeliac DiseaseChloridesCyclic AMPCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDuodenumHumansMaleProtonsRatsRats, Sprague-DawleySecond Messenger SystemsVacuolar Proton-Translocating ATPasesWaterConceptsCystic fibrosis transmembrane conductance regulatorV-ATPaseProton-pumping vacuolar ATPaseApical membraneDistinct membrane domainsFibrosis transmembrane conductance regulatorRat Brunner's glandsTransmembrane conductance regulatorSodium bicarbonate cotransporterAnion transportersApical traffickingMembrane domainsChloride cotransporter 1Secretion of glycoproteinsVacuolar ATPaseSecond messenger activationTransporter localizationConductance regulatorSubcellular distributionAquaporin 5 water channelFunctional transportPKA inhibitorAntimicrobial peptidesTransportersBasolateral membraneCFTR and V‐ATPase trafficking and function in the intestinal brush border membrane
Collaco A, Geibel P, Kravtsov D, Ghantaphang S, Lee B, Geibel J, Ameen N. CFTR and V‐ATPase trafficking and function in the intestinal brush border membrane. The FASEB Journal 2013, 27: 913.10-913.10. DOI: 10.1096/fasebj.27.1_supplement.913.10.Peer-Reviewed Original ResearchES cellsV-ATPaseMulti-subunit proton pumpCystic fibrosis transmembrane conductance regulator (CFTR) chloride channelV-ATPase traffickingIntestinal brush border membraneBrush border membraneBasolateral domainPlasma membraneVacuolar ATPaseE subunitBorder membraneCAMP stimulationProton pumpCAMP activationDistinct domainsFunctional synergyApical membraneCFTRChloride channelsProton effluxSubunitsMouse intestineTransportersE interaction