2020
Intestinal TMEM16A function as a luminal chloride channel
Kazi M, Saha T, Aoun J, Hayashi M, Sheikh I, Leblanc N, Sarkar P, Ameen N, Woodward O. Intestinal TMEM16A function as a luminal chloride channel. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.06115.Peer-Reviewed Original ResearchMouse intestinal tissueIntestinal tissueHuman colonic tissueColonic tissueLuminal expressionLuminal ClTMEM16A protein expressionIntestinal epithelial tissueAvailable polyclonal antibodyLuminal membraneWestern blot analysisTMEM16A functionSerosal applicationAgonist carbacholDistal colonCarbacholMRNA expressionColonWestern blotMouse intestineT84 cellsHigh expressionHuman colonProtein expressionTMEM16A protein
2017
AP2 α modulates cystic fibrosis transmembrane conductance regulator function in the human intestine
Kumari V, Desai S, Ameen NA. AP2 α modulates cystic fibrosis transmembrane conductance regulator function in the human intestine. Journal Of Cystic Fibrosis 2017, 16: 327-334. PMID: 28438500, PMCID: PMC5502754, DOI: 10.1016/j.jcf.2017.03.012.Peer-Reviewed Original Research
2016
Identification of intestinal ion transport defects in microvillus inclusion disease
Kravtsov DV, Ahsan MK, Kumari V, van Ijzendoorn SC, Reyes-Mugica M, Kumar A, Gujral T, Dudeja PK, Ameen NA. Identification of intestinal ion transport defects in microvillus inclusion disease. AJP Gastrointestinal And Liver Physiology 2016, 311: g142-g155. PMID: 27229121, PMCID: PMC4967175, DOI: 10.1152/ajpgi.00041.2016.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCaco-2 CellsChloride-Bicarbonate AntiportersCystic Fibrosis Transmembrane Conductance RegulatorEnterocytesGene Expression RegulationHumansIon TransportJejunumMalabsorption SyndromesMembrane Transport ProteinsMicrovilliMucolipidosesMyosin Heavy ChainsMyosin Type VPhenotypePhosphoproteinsRNA InterferenceSignal TransductionSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSulfate TransportersTranscription FactorsTransfectionYAP-Signaling ProteinsConceptsMicrovillus inclusion diseaseStool lossVillus atrophyInclusion diseaseEnterocyte maturationMicrovillus inclusionsIntestinal fluid transportIntestinal cell modelIon transport defectImmunohistochemical stainingSecretory diarrheaBrush border defectsImmature enterocytesC2BBe cellsT84 cellsElectrophysiological approachesCFTR ion transportBB membraneLoss of MYO5BEnterocytesFunctional CFTRDiarrheaAtrophyFunction mutationsCFTR localization