2017
Linaclotide activates guanylate cyclase‐C/cGMP/protein kinase‐II‐dependent trafficking of CFTR in the intestine
Ahsan K, Tchernychev B, Kessler MM, Solinga RM, Arthur D, Linde CI, Silos‐Santiago I, Hannig G, Ameen NA. Linaclotide activates guanylate cyclase‐C/cGMP/protein kinase‐II‐dependent trafficking of CFTR in the intestine. Physiological Reports 2017, 5: e13299. PMID: 28592587, PMCID: PMC5471438, DOI: 10.14814/phy2.13299.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell Line, TumorCell MembraneCyclic AMP-Dependent Protein KinasesCyclic GMPCyclic GMP-Dependent Protein Kinase Type IICystic Fibrosis Transmembrane Conductance RegulatorGuanylyl Cyclase C AgonistsHumansIntestinal MucosaMalePeptidesProtein TransportRatsRats, Sprague-DawleyReceptors, Guanylate Cyclase-CoupledSignal TransductionConceptsRat intestinal loopsLinaclotide treatmentFluid secretionIntestinal loopsCystic fibrosis transmembrane conductance regulatorCell surfaceFibrosis transmembrane conductance regulatorCell surface traffickingChronic idiopathic constipationIrritable bowel syndromeTransmembrane conductance regulatorIntestinal fluid secretionCell surface translocationReceptor guanylyl cyclaseHuman intestinal tissueCaco-2BBe cellsCFTR traffickingCFTR pathwaySubapical compartmentCellular signalingCGMP/PKGProtein kinaseSurface biotinylationIdiopathic constipationPathway components
2013
Role of Myosin1a in regulated exocytosis of CFTR in villus enterocytes
Hoekstra N, Kravtsov D, Mooseker M, Ameen N. Role of Myosin1a in regulated exocytosis of CFTR in villus enterocytes. The FASEB Journal 2013, 27: 913.11-913.11. DOI: 10.1096/fasebj.27.1_supplement.913.11.Peer-Reviewed Original ResearchSurface biotinylationRegulated exocytosisCFTR trafficSurface CFTRSubapical endosomesBrush border membraneMembrane traffickingCFTR deliveryKD cellsKnockdown cellsCFTR channelsCFTR distributionVillus enterocytesMolecular mechanismsEnterocyte brush border membraneCFTRBiotinylationConfocal microscopyEndosomesIntestinal brush border membraneMouse intestineExocytosisCGMP agonistMyo1aBorder membrane