2022
Serum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID)
Ahsan K, dos Reis D, Barbieri A, Sumigray K, Nottoli T, Salas P, Ameen N. Serum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID). The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.r5730.Peer-Reviewed Original ResearchMicrovillus inclusion diseaseGlucocorticoid-inducible kinase 1Severe diarrheaF miceInclusion diseaseCystic fibrosis transmembrane conductance regulatorFluid secretionWorsening of diarrheaMicrovillus inclusionsPhosphorylation of PDK1Kinase 1Intestinal lysatesSGK1 pathwayCarbohydrate malabsorptionER miceTamoxifen inductionBeta-ENaCDiarrheaDiarrheal diseaseSmall intestineMalabsorptionMiceDCKO miceGlucose lossPhosphorylation of SGK1
2018
Establishment of Microvillus Inclusion Disease (MVID) human models using small intestinal enteroids and embryonic stem cells
Figueroa‐Hall L, Ameen N. Establishment of Microvillus Inclusion Disease (MVID) human models using small intestinal enteroids and embryonic stem cells. The FASEB Journal 2018, 32: 750.31-750.31. DOI: 10.1096/fasebj.2018.32.1_supplement.750.31.Peer-Reviewed Original ResearchMicrovillus inclusion diseaseHuman intestinal modelSmall intestineIntestinal modelSeverity of illnessIntestinal organoidsSmall intestinal modelProximal small intestineCancer-derived cell linesFull-text articlesDevelopment of therapiesStem cellsIntestinal cell modelSmall intestinal organoidsIntestinal enteroidsInclusion diseasePathognomonic featuresSecretory diarrheaEpithelial diseaseHuman embryonic stem cellsIntestinal tissueIntestine resultsTransporter traffickingAppropriate research modelsLentiviral transfection
2013
Characterization of CFTR High Expresser (CHE) cells of the small intestine
Jakab R, Ameen N. Characterization of CFTR High Expresser (CHE) cells of the small intestine. The FASEB Journal 2013, 27: 913.12-913.12. DOI: 10.1096/fasebj.27.1_supplement.913.12.Peer-Reviewed Original Research
2009
T1744 Cell-Specific Functional Expression and Regulation of cAMP, Calcium and Acid-Stimulated Chloride and Bicarbonate Secretion in the Proximal Small Intestine
Jakab R, Collaco A, Ameen N. T1744 Cell-Specific Functional Expression and Regulation of cAMP, Calcium and Acid-Stimulated Chloride and Bicarbonate Secretion in the Proximal Small Intestine. Gastroenterology 2009, 136: a-570-a-571. DOI: 10.1016/s0016-5085(09)62625-1.Peer-Reviewed Original Research
2000
Cellular localization of the cystic fibrosis transmembrane conductance regulator in mouse intestinal tract
Ameen N, Alexis J, Salas P. Cellular localization of the cystic fibrosis transmembrane conductance regulator in mouse intestinal tract. Histochemistry And Cell Biology 2000, 114: 69-75. PMID: 10959824, DOI: 10.1007/s004180000164.Peer-Reviewed Original ResearchConceptsMouse intestinal tractCystic fibrosisIntestinal tractCystic fibrosis transmembrane conductance regulatorSmall intestinal obstructionSevere gastrointestinal diseasePathophysiology of CFMouse model systemFibrosis transmembrane conductance regulatorVillus distributionTransmembrane conductance regulatorIntestinal obstructionDifferent phenotypic expressionsCF intestineGastrointestinal diseasesBicarbonate secretionTransgenic miceHuman CF diseaseSmall intestineDisease expressionImmunoblot techniqueLethal obstructionMouse intestineMiceConductance regulator
1995
A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator
Ameen N, Ardito T, Kashgarian M, Marino C. A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator. Gastroenterology 1995, 108: 1016-1023. PMID: 7535272, DOI: 10.1016/0016-5085(95)90198-1.Peer-Reviewed Original ResearchConceptsProximal small intestineCrypt epithelial cellsVillus cellsSmall intestineHuman proximal small intestineCystic fibrosis transmembrane conductance regulatorEpithelial cellsSurface marker expressionFibrosis transmembrane conductance regulatorBrush border sucraseTransmembrane conductance regulatorIntestinal villus cellsBACKGROUND/Marker antibodiesMarker expressionUnique subsetVillus enterocytesConductance regulatorIntestineLactase expressionBrush border