2024
354 Elucidating the role of signaling and stress on CFTR and its contribution to diarrhea in SGK1 knockout mouse intestine
Muiler C, Santos A, Dastoor P, Jin J, dos Reis D, Hoque K, Ameen N. 354 Elucidating the role of signaling and stress on CFTR and its contribution to diarrhea in SGK1 knockout mouse intestine. Journal Of Cystic Fibrosis 2024, 23: s185. DOI: 10.1016/s1569-1993(24)01194-9.Peer-Reviewed Original ResearchMouse intestine
2021
Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner
Saha T, Aoun J, Hayashi M, Ali I, Sarkar P, Bag PK, Leblanc N, Ameen N, Woodward OM, Hoque KM. Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner. Biochemistry And Biophysics Reports 2021, 25: 100912. PMID: 33537462, PMCID: PMC7838733, DOI: 10.1016/j.bbrep.2021.100912.Peer-Reviewed Original Research
2020
Intestinal TMEM16A function as a luminal chloride channel
Kazi M, Saha T, Aoun J, Hayashi M, Sheikh I, Leblanc N, Sarkar P, Ameen N, Woodward O. Intestinal TMEM16A function as a luminal chloride channel. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.06115.Peer-Reviewed Original ResearchMouse intestinal tissueIntestinal tissueHuman colonic tissueColonic tissueLuminal expressionLuminal ClTMEM16A protein expressionIntestinal epithelial tissueAvailable polyclonal antibodyLuminal membraneWestern blot analysisTMEM16A functionSerosal applicationAgonist carbacholDistal colonCarbacholMRNA expressionColonWestern blotMouse intestineT84 cellsHigh expressionHuman colonProtein expressionTMEM16A protein
2013
Role of Myosin1a in regulated exocytosis of CFTR in villus enterocytes
Hoekstra N, Kravtsov D, Mooseker M, Ameen N. Role of Myosin1a in regulated exocytosis of CFTR in villus enterocytes. The FASEB Journal 2013, 27: 913.11-913.11. DOI: 10.1096/fasebj.27.1_supplement.913.11.Peer-Reviewed Original ResearchSurface biotinylationRegulated exocytosisCFTR trafficSurface CFTRSubapical endosomesBrush border membraneMembrane traffickingCFTR deliveryKD cellsKnockdown cellsCFTR channelsCFTR distributionVillus enterocytesMolecular mechanismsEnterocyte brush border membraneCFTRBiotinylationConfocal microscopyEndosomesIntestinal brush border membraneMouse intestineExocytosisCGMP agonistMyo1aBorder membraneCFTR and V‐ATPase trafficking and function in the intestinal brush border membrane
Collaco A, Geibel P, Kravtsov D, Ghantaphang S, Lee B, Geibel J, Ameen N. CFTR and V‐ATPase trafficking and function in the intestinal brush border membrane. The FASEB Journal 2013, 27: 913.10-913.10. DOI: 10.1096/fasebj.27.1_supplement.913.10.Peer-Reviewed Original ResearchES cellsV-ATPaseMulti-subunit proton pumpCystic fibrosis transmembrane conductance regulator (CFTR) chloride channelV-ATPase traffickingIntestinal brush border membraneBrush border membraneBasolateral domainPlasma membraneVacuolar ATPaseE subunitBorder membraneCAMP stimulationProton pumpCAMP activationDistinct domainsFunctional synergyApical membraneCFTRChloride channelsProton effluxSubunitsMouse intestineTransportersE interaction
2000
Cellular localization of the cystic fibrosis transmembrane conductance regulator in mouse intestinal tract
Ameen N, Alexis J, Salas P. Cellular localization of the cystic fibrosis transmembrane conductance regulator in mouse intestinal tract. Histochemistry And Cell Biology 2000, 114: 69-75. PMID: 10959824, DOI: 10.1007/s004180000164.Peer-Reviewed Original ResearchConceptsMouse intestinal tractCystic fibrosisIntestinal tractCystic fibrosis transmembrane conductance regulatorSmall intestinal obstructionSevere gastrointestinal diseasePathophysiology of CFMouse model systemFibrosis transmembrane conductance regulatorVillus distributionTransmembrane conductance regulatorIntestinal obstructionDifferent phenotypic expressionsCF intestineGastrointestinal diseasesBicarbonate secretionTransgenic miceHuman CF diseaseSmall intestineDisease expressionImmunoblot techniqueLethal obstructionMouse intestineMiceConductance regulator