2024
Localization and function of humanized F508del-CFTR in mouse intestine following activation of serum glucocorticoid kinase 1 and Trikafta
Dastoor P, Muiler C, Garrison A, Egan M, Carlos Dos Reis D, Santos A, Ameen N. Localization and function of humanized F508del-CFTR in mouse intestine following activation of serum glucocorticoid kinase 1 and Trikafta. European Journal Of Pharmacology 2024, 978: 176771. PMID: 38925289, DOI: 10.1016/j.ejphar.2024.176771.Peer-Reviewed Original ResearchSerum glucocorticoid kinase 1Glucocorticoid kinase 1F508del-CFTRCystic fibrosisMouse modelF508del-CFTR miceCFTR-expressing epithelial cellsCF mouse modelsHumanized mouse modelTreatment of ratsIntestinal diseaseIntestinal segmentsKinase 1CFTR modulatorsCFTR mutationsCF patientsTrikaftaDex treatmentLung diseaseEfficacy of compoundsDelta F508CFTRCombined treatmentEpithelial cellsLoss of function
2010
T1837 The Vacuolar ATPase Associates With CFTR in Apical Endocytic and Recycling Vesicles and Undergoes cAMP Regulated Trafficking in Intestinal Epithelial Cells
Collaco A, Jakab R, Gorelick F, Ameen N. T1837 The Vacuolar ATPase Associates With CFTR in Apical Endocytic and Recycling Vesicles and Undergoes cAMP Regulated Trafficking in Intestinal Epithelial Cells. Gastroenterology 2010, 138: s-589. DOI: 10.1016/s0016-5085(10)62715-1.Peer-Reviewed Original Research
2001
Anomalous apical plasma membrane phenotype in CK8-deficient mice indicates a novel role for intermediate filaments in the polarization of simple epithelia
Ameen N, Figueroa Y, Salas P. Anomalous apical plasma membrane phenotype in CK8-deficient mice indicates a novel role for intermediate filaments in the polarization of simple epithelia. Journal Of Cell Science 2001, 114: 563-575. PMID: 11171325, DOI: 10.1242/jcs.114.3.563.Peer-Reviewed Original ResearchConceptsIntermediate filamentsPolarized epithelial cellsApical membrane proteinsEpithelial cellsSyntaxin 3Apical domainFemale sterilityMembrane proteinsApical markerNovel functionGamma-tubulinNovel roleApical poleSimple epitheliaCell typesColorectal hyperplasiaCK intermediate filamentsNull micePhenotypeBasolateral levelsNecrotic cellsMembrane phenotypeCellsFilamentsCytoplasm of enterocytes
2000
Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic
Ameen N, van Donselaar E, Posthuma G, de Jonge H, McLaughlin G, Geuze H, Marino C, Peters P. Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic. Histochemistry And Cell Biology 2000, 114: 219-228. PMID: 11083465, DOI: 10.1007/s004180000167.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorVesicle trafficSubcellular distributionVesicle insertionCAMP stimulationCAMP-activated chloride channelCryoimmunogold electron microscopyFibrosis transmembrane conductance regulatorApical plasma membraneTransmembrane conductance regulatorCultured intestinal cellsCFTR regulationCHE cellsPhysiologic roleVesicular compartmentsPlasma membraneApical redistributionConductance regulatorSubapical vesiclesCellular distributionChloride channelsSecretory cellsIntestinal cellsEpithelial cellsCellsMicrovillus Inclusion Disease: A Genetic Defect Affecting Apical Membrane Protein Traffic in Intestinal Epithelium
Ameen N, Salas P. Microvillus Inclusion Disease: A Genetic Defect Affecting Apical Membrane Protein Traffic in Intestinal Epithelium. Traffic 2000, 1: 76-83. PMID: 11208062, DOI: 10.1034/j.1600-0854.2000.010111.x.Peer-Reviewed Original ResearchConceptsMicrovillus inclusion diseaseApical membrane trafficMembrane protein trafficVacuolar apical compartmentApical membrane markersMicrovillus inclusionsF-actin layerBasolateral proteinsFirst genetic defectMembrane trafficTissue culture epithelial cellsProtein trafficApical exocytosisLater stepsApical membraneMembrane markersTerminal webImmunofluorescence analysisApical cytoplasmGenetic defectsEpithelial cellsProteinApical compartmentIntestinal epitheliumNormal microtubules
1999
CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo
Ameen N, Martensson B, Bourguinon L, Marino C, Isenberg J, McLaughlin G. CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo. Journal Of Cell Science 1999, 112: 887-894. PMID: 10036238, DOI: 10.1242/jcs.112.6.887.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulator (CFTR) channelVillus epithelial cellsApical surfaceApical plasma membraneAnti-CFTR antibodiesQuantitative confocal microscopyNew protein synthesisApical membrane insertionIntestinal villus epithelial cellsEpithelial cellsMembrane CFTRMembrane insertionApical cytoskeletonNormal CFTRCHE cellsPlasma membraneAbsence of cycloheximideSubcellular redistributionCFTR channelsC-terminusCAMP stimulationIntracellular cAMP levelsProtein synthesisChannel insertionCFTR function
1995
A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator
Ameen N, Ardito T, Kashgarian M, Marino C. A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator. Gastroenterology 1995, 108: 1016-1023. PMID: 7535272, DOI: 10.1016/0016-5085(95)90198-1.Peer-Reviewed Original ResearchConceptsProximal small intestineCrypt epithelial cellsVillus cellsSmall intestineHuman proximal small intestineCystic fibrosis transmembrane conductance regulatorEpithelial cellsSurface marker expressionFibrosis transmembrane conductance regulatorBrush border sucraseTransmembrane conductance regulatorIntestinal villus cellsBACKGROUND/Marker antibodiesMarker expressionUnique subsetVillus enterocytesConductance regulatorIntestineLactase expressionBrush border