2023
CFTR High Expresser Cells in cystic fibrosis and intestinal diseases
dos Reis D, Dastoor P, Santos A, Sumigray K, Ameen N. CFTR High Expresser Cells in cystic fibrosis and intestinal diseases. Heliyon 2023, 9: e14568. PMID: 36967909, PMCID: PMC10031467, DOI: 10.1016/j.heliyon.2023.e14568.Peer-Reviewed Original ResearchCystic fibrosisIntestinal diseaseCystic fibrosis transmembrane conductance regulatorQuality of lifeIntestinal dysfunctionGastrointestinal diseasesIntestinal physiologyFibrosisDiseasePathophysiologyFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCFTR leadConductance regulator
2022
Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID)
Ahsan K, dos Reis DC, Barbieri A, Sumigray KD, Nottoli T, Salas PJ, Ameen NA. Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID). Journal Of Clinical Medicine 2022, 11: 4179. PMID: 35887942, PMCID: PMC9319011, DOI: 10.3390/jcm11144179.Peer-Reviewed Original ResearchMicrovillus inclusion diseaseSevere diarrheaInclusion diseaseFluid secretionCystic fibrosis transmembrane conductance regulatorDouble knockout miceMicrovillus inclusionsGlucocorticoid-inducible kinase 1Carbohydrate malabsorptionKnockout miceIntestinal carbohydrateDiarrheaDiarrheal diseaseMalabsorptionMiceVillus enterocytesDiseaseLoss of MYO5BFunction mutationsApical cystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorKinase 1SecretionIntestineTransmembrane conductance regulatorSerum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID)
Ahsan K, dos Reis D, Barbieri A, Sumigray K, Nottoli T, Salas P, Ameen N. Serum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID). The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.r5730.Peer-Reviewed Original ResearchMicrovillus inclusion diseaseGlucocorticoid-inducible kinase 1Severe diarrheaF miceInclusion diseaseCystic fibrosis transmembrane conductance regulatorFluid secretionWorsening of diarrheaMicrovillus inclusionsPhosphorylation of PDK1Kinase 1Intestinal lysatesSGK1 pathwayCarbohydrate malabsorptionER miceTamoxifen inductionBeta-ENaCDiarrheaDiarrheal diseaseSmall intestineMalabsorptionMiceDCKO miceGlucose lossPhosphorylation of SGK1
2017
Linaclotide activates guanylate cyclase‐C/cGMP/protein kinase‐II‐dependent trafficking of CFTR in the intestine
Ahsan K, Tchernychev B, Kessler MM, Solinga RM, Arthur D, Linde CI, Silos‐Santiago I, Hannig G, Ameen NA. Linaclotide activates guanylate cyclase‐C/cGMP/protein kinase‐II‐dependent trafficking of CFTR in the intestine. Physiological Reports 2017, 5: e13299. PMID: 28592587, PMCID: PMC5471438, DOI: 10.14814/phy2.13299.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell Line, TumorCell MembraneCyclic AMP-Dependent Protein KinasesCyclic GMPCyclic GMP-Dependent Protein Kinase Type IICystic Fibrosis Transmembrane Conductance RegulatorGuanylyl Cyclase C AgonistsHumansIntestinal MucosaMalePeptidesProtein TransportRatsRats, Sprague-DawleyReceptors, Guanylate Cyclase-CoupledSignal TransductionConceptsRat intestinal loopsLinaclotide treatmentFluid secretionIntestinal loopsCystic fibrosis transmembrane conductance regulatorCell surfaceFibrosis transmembrane conductance regulatorCell surface traffickingChronic idiopathic constipationIrritable bowel syndromeTransmembrane conductance regulatorIntestinal fluid secretionCell surface translocationReceptor guanylyl cyclaseHuman intestinal tissueCaco-2BBe cellsCFTR traffickingCFTR pathwaySubapical compartmentCellular signalingCGMP/PKGProtein kinaseSurface biotinylationIdiopathic constipationPathway components
2013
Regulated traffic of anion transporters in mammalian Brunner's glands: a role for water and fluid transport
Collaco AM, Jakab RL, Hoekstra NE, Mitchell KA, Brooks A, Ameen NA. Regulated traffic of anion transporters in mammalian Brunner's glands: a role for water and fluid transport. AJP Gastrointestinal And Liver Physiology 2013, 305: g258-g275. PMID: 23744739, PMCID: PMC3742856, DOI: 10.1152/ajpgi.00485.2012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnion Transport ProteinsAquaporin 5BicarbonatesBiological TransportBrunner GlandsCarbacholCeliac DiseaseChloridesCyclic AMPCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDuodenumHumansMaleProtonsRatsRats, Sprague-DawleySecond Messenger SystemsVacuolar Proton-Translocating ATPasesWaterConceptsCystic fibrosis transmembrane conductance regulatorV-ATPaseProton-pumping vacuolar ATPaseApical membraneDistinct membrane domainsFibrosis transmembrane conductance regulatorRat Brunner's glandsTransmembrane conductance regulatorSodium bicarbonate cotransporterAnion transportersApical traffickingMembrane domainsChloride cotransporter 1Secretion of glycoproteinsVacuolar ATPaseSecond messenger activationTransporter localizationConductance regulatorSubcellular distributionAquaporin 5 water channelFunctional transportPKA inhibitorAntimicrobial peptidesTransportersBasolateral membrane
2000
Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic
Ameen N, van Donselaar E, Posthuma G, de Jonge H, McLaughlin G, Geuze H, Marino C, Peters P. Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic. Histochemistry And Cell Biology 2000, 114: 219-228. PMID: 11083465, DOI: 10.1007/s004180000167.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorVesicle trafficSubcellular distributionVesicle insertionCAMP stimulationCAMP-activated chloride channelCryoimmunogold electron microscopyFibrosis transmembrane conductance regulatorApical plasma membraneTransmembrane conductance regulatorCultured intestinal cellsCFTR regulationCHE cellsPhysiologic roleVesicular compartmentsPlasma membraneApical redistributionConductance regulatorSubapical vesiclesCellular distributionChloride channelsSecretory cellsIntestinal cellsEpithelial cellsCellsCellular localization of the cystic fibrosis transmembrane conductance regulator in mouse intestinal tract
Ameen N, Alexis J, Salas P. Cellular localization of the cystic fibrosis transmembrane conductance regulator in mouse intestinal tract. Histochemistry And Cell Biology 2000, 114: 69-75. PMID: 10959824, DOI: 10.1007/s004180000164.Peer-Reviewed Original ResearchConceptsMouse intestinal tractCystic fibrosisIntestinal tractCystic fibrosis transmembrane conductance regulatorSmall intestinal obstructionSevere gastrointestinal diseasePathophysiology of CFMouse model systemFibrosis transmembrane conductance regulatorVillus distributionTransmembrane conductance regulatorIntestinal obstructionDifferent phenotypic expressionsCF intestineGastrointestinal diseasesBicarbonate secretionTransgenic miceHuman CF diseaseSmall intestineDisease expressionImmunoblot techniqueLethal obstructionMouse intestineMiceConductance regulator
1995
A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator
Ameen N, Ardito T, Kashgarian M, Marino C. A unique subset of rat and human intestinal villus cells express the cystic fibrosis transmembrane conductance regulator. Gastroenterology 1995, 108: 1016-1023. PMID: 7535272, DOI: 10.1016/0016-5085(95)90198-1.Peer-Reviewed Original ResearchConceptsProximal small intestineCrypt epithelial cellsVillus cellsSmall intestineHuman proximal small intestineCystic fibrosis transmembrane conductance regulatorEpithelial cellsSurface marker expressionFibrosis transmembrane conductance regulatorBrush border sucraseTransmembrane conductance regulatorIntestinal villus cellsBACKGROUND/Marker antibodiesMarker expressionUnique subsetVillus enterocytesConductance regulatorIntestineLactase expressionBrush border