2024
Localization and function of humanized F508del-CFTR in mouse intestine following activation of serum glucocorticoid kinase 1 and Trikafta
Dastoor P, Muiler C, Garrison A, Egan M, Carlos Dos Reis D, Santos A, Ameen N. Localization and function of humanized F508del-CFTR in mouse intestine following activation of serum glucocorticoid kinase 1 and Trikafta. European Journal Of Pharmacology 2024, 978: 176771. PMID: 38925289, DOI: 10.1016/j.ejphar.2024.176771.Peer-Reviewed Original ResearchSerum glucocorticoid kinase 1Glucocorticoid kinase 1F508del-CFTRCystic fibrosisMouse modelF508del-CFTR miceCFTR-expressing epithelial cellsCF mouse modelsHumanized mouse modelTreatment of ratsIntestinal diseaseIntestinal segmentsKinase 1CFTR modulatorsCFTR mutationsCF patientsTrikaftaDex treatmentLung diseaseEfficacy of compoundsDelta F508CFTRCombined treatmentEpithelial cellsLoss of function
2017
AP2 α modulates cystic fibrosis transmembrane conductance regulator function in the human intestine
Kumari V, Desai S, Ameen NA. AP2 α modulates cystic fibrosis transmembrane conductance regulator function in the human intestine. Journal Of Cystic Fibrosis 2017, 16: 327-334. PMID: 28438500, PMCID: PMC5502754, DOI: 10.1016/j.jcf.2017.03.012.Peer-Reviewed Original Research
2013
Functional vacuolar ATPase (V-ATPase) proton pumps traffic to the enterocyte brush border membrane and require CFTR
Collaco AM, Geibel P, Lee BS, Geibel JP, Ameen NA. Functional vacuolar ATPase (V-ATPase) proton pumps traffic to the enterocyte brush border membrane and require CFTR. American Journal Of Physiology - Cell Physiology 2013, 305: c981-c996. PMID: 23986201, PMCID: PMC4109618, DOI: 10.1152/ajpcell.00067.2013.Peer-Reviewed Original ResearchConceptsV-ATPaseCystic fibrosis transmembrane conductance regulator (CFTR) channelV-ATPase complexV-ATPase functionBrush border membraneProton effluxRat Brunner's glandsIntestinal cellsCAMP/PKACaco-2BBe cellsBorder membraneApical domainCoimmunoprecipitation studiesCFTR distributionVacuolar ATPasesSubapical cytoplasmSpecific subunitsCAMP stimulationProton pumpCAMP treatmentEnterocyte brush border membraneSodium-hydrogen exchangerHydrogen exchangerApical membraneCFTRRole of Myosin1a in regulated exocytosis of CFTR in villus enterocytes
Hoekstra N, Kravtsov D, Mooseker M, Ameen N. Role of Myosin1a in regulated exocytosis of CFTR in villus enterocytes. The FASEB Journal 2013, 27: 913.11-913.11. DOI: 10.1096/fasebj.27.1_supplement.913.11.Peer-Reviewed Original ResearchSurface biotinylationRegulated exocytosisCFTR trafficSurface CFTRSubapical endosomesBrush border membraneMembrane traffickingCFTR deliveryKD cellsKnockdown cellsCFTR channelsCFTR distributionVillus enterocytesMolecular mechanismsEnterocyte brush border membraneCFTRBiotinylationConfocal microscopyEndosomesIntestinal brush border membraneMouse intestineExocytosisCGMP agonistMyo1aBorder membraneCFTR and V‐ATPase trafficking and function in the intestinal brush border membrane
Collaco A, Geibel P, Kravtsov D, Ghantaphang S, Lee B, Geibel J, Ameen N. CFTR and V‐ATPase trafficking and function in the intestinal brush border membrane. The FASEB Journal 2013, 27: 913.10-913.10. DOI: 10.1096/fasebj.27.1_supplement.913.10.Peer-Reviewed Original ResearchES cellsV-ATPaseMulti-subunit proton pumpCystic fibrosis transmembrane conductance regulator (CFTR) chloride channelV-ATPase traffickingIntestinal brush border membraneBrush border membraneBasolateral domainPlasma membraneVacuolar ATPaseE subunitBorder membraneCAMP stimulationProton pumpCAMP activationDistinct domainsFunctional synergyApical membraneCFTRChloride channelsProton effluxSubunitsMouse intestineTransportersE interaction
2010
T1837 The Vacuolar ATPase Associates With CFTR in Apical Endocytic and Recycling Vesicles and Undergoes cAMP Regulated Trafficking in Intestinal Epithelial Cells
Collaco A, Jakab R, Gorelick F, Ameen N. T1837 The Vacuolar ATPase Associates With CFTR in Apical Endocytic and Recycling Vesicles and Undergoes cAMP Regulated Trafficking in Intestinal Epithelial Cells. Gastroenterology 2010, 138: s-589. DOI: 10.1016/s0016-5085(10)62715-1.Peer-Reviewed Original Research
2008
S1771 Regulated Exocytosis and Fusion of Vesicle Associated CFTR to the Apical Plasma Membrane of the Intestine Is Mediated By the Snare Protein Syntaxin-3
Ameen N, Collaco A. S1771 Regulated Exocytosis and Fusion of Vesicle Associated CFTR to the Apical Plasma Membrane of the Intestine Is Mediated By the Snare Protein Syntaxin-3. Gastroenterology 2008, 134: a-266. DOI: 10.1016/s0016-5085(08)61239-1.Peer-Reviewed Original Research