2015
Mechanisms of chorioamnionitis‐associated preterm birth: interleukin‐1β inhibits progesterone receptor expression in decidual cells
Guzeloglu-Kayisli O, Kayisli UA, Semerci N, Basar M, Buchwalder LF, Buhimschi CS, Buhimschi IA, Arcuri F, Larsen K, Huang JS, Schatz F, Lockwood CJ. Mechanisms of chorioamnionitis‐associated preterm birth: interleukin‐1β inhibits progesterone receptor expression in decidual cells. The Journal Of Pathology 2015, 237: 423-434. PMID: 26175191, DOI: 10.1002/path.4589.Peer-Reviewed Original ResearchConceptsProgesterone receptor expressionPreterm birthDecidual cellsPR expressionIL-1βDC culturesReceptor expressionPR levelsIL-1β suppressionLower PR levelsFunctional progesterone withdrawalCOX-2 expressionRelease of cytokinesIL-1β treatmentDecidual prostaglandin productionP38 MAPK signaling cascadesCervical ripeningProgesterone withdrawalCOX inhibitorsImmunohistochemical stainingChorioamnionitisFetal membrane ruptureNF-kBProstaglandin productionPGF2α production
2014
Interferon-γ Protects First-Trimester Decidual Cells against Aberrant Matrix Metalloproteinases 1, 3, and 9 Expression in Preeclampsia
Lockwood CJ, Basar M, Kayisli UA, Guzeloglu-Kayisli O, Murk W, Wang J, De Paz N, Shapiro JP, Masch RJ, Semerci N, Huang SJ, Schatz F. Interferon-γ Protects First-Trimester Decidual Cells against Aberrant Matrix Metalloproteinases 1, 3, and 9 Expression in Preeclampsia. American Journal Of Pathology 2014, 184: 2549-2559. PMID: 25065683, PMCID: PMC4188280, DOI: 10.1016/j.ajpath.2014.05.025.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternDeciduaEnzyme-Linked Immunosorbent AssayFemaleHumansImmunohistochemistryInterferon-gammaKiller Cells, NaturalMacrophagesMatrix Metalloproteinase 1Matrix Metalloproteinase 3Matrix Metalloproteinase 9Matrix Metalloproteinases, SecretedPre-EclampsiaPregnancyPregnancy Trimester, FirstReal-Time Polymerase Chain ReactionConceptsMitogen-activated protein kinaseP38 mitogen-activated protein kinaseMitogen-activated protein kinase phosphorylationP38 mitogen-activated protein kinase phosphorylationExtracellular matrix proteinsProtein kinase phosphorylationHuman first trimester decidual cellsProtein kinaseReal-time quantitative RT-PCREVT invasionKinase phosphorylationQuantitative RT-PCRMatrix proteinsProteolytic cascadeDecidual cellsIntegrin receptorsExtravillous trophoblastsHuman extravillous trophoblastsIncomplete spiral arteryMMP-9Protein levelsProtein expressionMMP-1MMP-2Fibrillar collagen
2011
Bidirectional Interaction Between Unfolded-Protein-Response Key Protein HSPA5 and Estrogen Signaling in Human Endometrium1
Guzel E, Basar M, Ocak N, Arici A, Kayisli UA. Bidirectional Interaction Between Unfolded-Protein-Response Key Protein HSPA5 and Estrogen Signaling in Human Endometrium1. Biology Of Reproduction 2011, 85: 121-127. PMID: 21389343, DOI: 10.1095/biolreprod.110.089532.Peer-Reviewed Original ResearchConceptsHSPA5 expressionHuman endometriumMenstrual cycleStromal cellsUnfolded protein responseNormal human endometriumEndometrial glandularEstradiol levelsHuman Endometrium1Possible inverse correlationIshikawa cellsEstrogen signalingLocal paracrineEndometriumSteroid hormonesAutocrine factorHeat shock 70EstrogenInverse correlationBidirectional regulationExpression levelsBidirectional interactionsShock 70Dynamic tissueHSPA5
2010
Preeclampsia-related increase of interleukin-11 expression in human decidual cells
Basar M, Yen CF, Buchwalder LF, Murk W, Huang SJ, Godlewski K, Kocamaz E, Arda O, Schatz F, Lockwood CJ, Kayisli UA. Preeclampsia-related increase of interleukin-11 expression in human decidual cells. Reproduction 2010, 140: 605-612. PMID: 20668109, PMCID: PMC3795512, DOI: 10.1530/rep-10-0263.Peer-Reviewed Original ResearchMeSH KeywordsAdultDeciduaEstradiolFemaleHumansImidazolesImmunohistochemistryInterleukin-11Interleukin-1betaNaphthalenesNF-kappa BP38 Mitogen-Activated Protein KinasesPre-EclampsiaPregnancyProtein Kinase CPyridinesReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStatistics, NonparametricTumor Necrosis Factor-alphaConceptsFirst trimester decidual cellsDecidual cellsPlacental sectionsLeukocyte-free first trimester decidual cellsGestational age-matched controlsProtein kinase CMaternal-placental interfaceUteroplacental blood flowPathogenesis of preeclampsiaAnti-inflammatory processesCultured decidual cellsTumor necrosis factorAge-matched controlsHuman decidual cellsQuantitative RT-PCRSystemic inflammationNecrosis factorPreeclampsiaBlood flowP38 MAP kinaseIL11 expressionNuclear factorStimulatory effectIL11RT-PCR