2018
Development and Application of FASA, a Model for Quantifying Fatty Acid Metabolism Using Stable Isotope Labeling
Argus J, Wilks M, Zhou Q, Hsieh W, Khialeeva E, Hoi X, Bui V, Xu S, Yu A, Wang E, Herschman H, Williams K, Bensinger S. Development and Application of FASA, a Model for Quantifying Fatty Acid Metabolism Using Stable Isotope Labeling. Cell Reports 2018, 25: 2919-2934.e8. PMID: 30517876, PMCID: PMC6432944, DOI: 10.1016/j.celrep.2018.11.041.Peer-Reviewed Original ResearchConceptsFatty acid metabolismHomeostasis of fatty acidsCellular fatty acid contentCellular fatty acid metabolismAcid metabolismMovement of fatty acidsPolyunsaturated fatty acid poolStable isotope labelingFatty acidsFatty acid homeostasisElongation pathwayFatty acid poolAcid homeostasisMetabolic stepsContribution of synthesisAcid poolPro-inflammatory stimuliFatty acid contentIsotope labelingInflammatory signalingAcid contentMetabolismCellsElongationAcid
2015
Limiting Cholesterol Biosynthetic Flux Spontaneously Engages Type I IFN Signaling
York AG, Williams KJ, Argus JP, Zhou QD, Brar G, Vergnes L, Gray EE, Zhen A, Wu NC, Yamada DH, Cunningham CR, Tarling EJ, Wilks MQ, Casero D, Gray DH, Yu AK, Wang ES, Brooks DG, Sun R, Kitchen SG, Wu TT, Reue K, Stetson DB, Bensinger SJ. Limiting Cholesterol Biosynthetic Flux Spontaneously Engages Type I IFN Signaling. Cell 2015, 163: 1716-1729. PMID: 26686653, PMCID: PMC4783382, DOI: 10.1016/j.cell.2015.11.045.Peer-Reviewed Original ResearchConceptsImport of cholesterolI interferonType I IFNsSTING-dependent mannerCholesterol biosynthetic pathwayI IFNsCombination of biosynthesisBiosynthetic fluxBiosynthetic pathwayLong-chain fatty acidsIsotope tracer analysisMetabolic shiftMetabolic pathwaysType I interferonCholesterol biosynthesisLipid requirementsChain fatty acidsInnate immunityBiosynthesisFatty acidsPool sizePathwayMechanistic studiesViral challengeFree cholesterol