2024
Fasting-mimicking diet causes hepatic and blood markers changes indicating reduced biological age and disease risk
Brandhorst S, Levine M, Wei M, Shelehchi M, Morgan T, Nayak K, Dorff T, Hong K, Crimmins E, Cohen P, Longo V. Fasting-mimicking diet causes hepatic and blood markers changes indicating reduced biological age and disease risk. Nature Communications 2024, 15: 1309. PMID: 38378685, PMCID: PMC10879164, DOI: 10.1038/s41467-024-45260-9.Peer-Reviewed Original ResearchConceptsMultiple cardiometabolic risk factorsAssociated with reduced insulin resistanceCardiometabolic risk factorsFasting-mimicking dietImmune system agingRandomized clinical trialsAnalysis of blood samplesAutoimmune cellsBiological ageClinical trialsReduce inflammationMarker changesRisk factorsHepatic fatInsulin resistanceAdult study participantsBlood samplesNormal cellsWeight lossReducing biological ageBiomarker of biological agingDamaged cellsStudy participantsDisease riskAge
2022
Tick tock, tick tock: Mouse culture and tissue aging captured by an epigenetic clock
Minteer C, Morselli M, Meer M, Cao J, Higgins‐Chen A, Lang SM, Pellegrini M, Yan Q, Levine ME. Tick tock, tick tock: Mouse culture and tissue aging captured by an epigenetic clock. Aging Cell 2022, 21: e13553. PMID: 35104377, PMCID: PMC8844113, DOI: 10.1111/acel.13553.Peer-Reviewed Original ResearchConceptsMouse embryonic fibroblastsDNA methylationEpigenetic agingImportant chromatin regulatorsPolycomb group (PcG) factorsAnti-aging interventionsChromatin regulatorsEmbryonic fibroblastsCellular senescenceTissue agingCellular agingEpigenetic clocksMultiple tissuesMouse tissuesCaloric restrictionMechanistic insightsAging changesKidney fibroblastsReduced representationTime pointsPhysiological agingMouse culturesSuch alterationsTick-TockTissueEpigenetic aging of the demographically non-aging naked mole-rat
Kerepesi C, Meer MV, Ablaeva J, Amoroso VG, Lee SG, Zhang B, Gerashchenko MV, Trapp A, Yim SH, Lu AT, Levine ME, Seluanov A, Horvath S, Park TJ, Gorbunova V, Gladyshev VN. Epigenetic aging of the demographically non-aging naked mole-rat. Nature Communications 2022, 13: 355. PMID: 35039495, PMCID: PMC8763950, DOI: 10.1038/s41467-022-27959-9.Peer-Reviewed Original Research
2020
Reprogramming to recover youthful epigenetic information and restore vision
Lu Y, Brommer B, Tian X, Krishnan A, Meer M, Wang C, Vera DL, Zeng Q, Yu D, Bonkowski MS, Yang JH, Zhou S, Hoffmann EM, Karg MM, Schultz MB, Kane AE, Davidsohn N, Korobkina E, Chwalek K, Rajman LA, Church GM, Hochedlinger K, Gladyshev VN, Horvath S, Levine ME, Gregory-Ksander MS, Ksander BR, He Z, Sinclair DA. Reprogramming to recover youthful epigenetic information and restore vision. Nature 2020, 588: 124-129. PMID: 33268865, PMCID: PMC7752134, DOI: 10.1038/s41586-020-2975-4.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAxonsCell Line, TumorCell SurvivalCellular ReprogrammingDependovirusDioxygenasesDisease Models, AnimalDNA MethylationDNA-Binding ProteinsEpigenesis, GeneticEyeFemaleGenetic VectorsGlaucomaHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMiceMice, Inbred C57BLNerve RegenerationOctamer Transcription Factor-3Optic Nerve InjuriesProto-Oncogene ProteinsRetinal Ganglion CellsSOXB1 Transcription FactorsTranscriptomeVision, OcularConceptsDNA methylation patternsMethylation patternsTissue functionCentral nervous systemGene expression patternsCause of agingEpigenetic noiseEpigenetic informationDNA methylationEctopic expressionExpression patternsKLF4 geneMouse retinal ganglion cellsMammalian tissuesRetinal ganglion cellsAged miceGanglion cellsVision lossTissue dysfunctionMouse modelCNS tissueAxon regenerationNervous systemDegenerative processOlder individualsMouse brain transcriptome responses to inhaled nanoparticulate matter differed by sex and APOE in Nrf2-Nfkb interactions
Haghani A, Cacciottolo M, Doty KR, D'Agostino C, Thorwald M, Safi N, Levine ME, Sioutas C, Town TC, Forman HJ, Zhang H, Morgan TE, Finch CE. Mouse brain transcriptome responses to inhaled nanoparticulate matter differed by sex and APOE in Nrf2-Nfkb interactions. ELife 2020, 9: e54822. PMID: 32579111, PMCID: PMC7314548, DOI: 10.7554/elife.54822.Peer-Reviewed Original Research
2018
Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
Yen K, Wan J, Mehta HH, Miller B, Christensen A, Levine ME, Salomon MP, Brandhorst S, Xiao J, Kim SJ, Navarrete G, Campo D, Harry GJ, Longo V, Pike CJ, Mack WJ, Hodis HN, Crimmins EM, Cohen P. Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans. Scientific Reports 2018, 8: 14212. PMID: 30242290, PMCID: PMC6154958, DOI: 10.1038/s41598-018-32616-7.Peer-Reviewed Original Research
2014
Low Protein Intake Is Associated with a Major Reduction in IGF-1, Cancer, and Overall Mortality in the 65 and Younger but Not Older Population
Levine ME, Suarez JA, Brandhorst S, Balasubramanian P, Cheng CW, Madia F, Fontana L, Mirisola MG, Guevara-Aguirre J, Wan J, Passarino G, Kennedy BK, Wei M, Cohen P, Crimmins EM, Longo VD. Low Protein Intake Is Associated with a Major Reduction in IGF-1, Cancer, and Overall Mortality in the 65 and Younger but Not Older Population. Cell Metabolism 2014, 19: 407-417. PMID: 24606898, PMCID: PMC3988204, DOI: 10.1016/j.cmet.2014.02.006.Peer-Reviewed Original ResearchMeSH KeywordsAgedAnimalsBreast NeoplasmsCarrier ProteinsCross-Sectional StudiesDiabetes MellitusDiet, Protein-RestrictedFemaleFollow-Up StudiesHumansInsulin-Like Growth Factor ILongevityMaleMelanomaMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutMiddle AgedNeoplasmsProportional Hazards ModelsSignal TransductionConceptsHigh protein intakeOverall mortalityProtein intakeCancer death riskProgression of breastLow protein intakeLow-protein dietHigh protein consumptionDiabetes mortalityAge-related diseasesDeath riskProtein restrictionIGF-1Melanoma tumorsMortalityMouse studiesOlder populationProtein dietOlder adultsIntakeMajor reductionProtein consumptionCancerLow proteinAge