2024
Detection of Chikungunya Virus RNA in Oral Fluid and Urine: An Alternative Approach to Diagnosis?
Jacob-Nascimento L, Portilho M, Anjos R, Moreira P, Stauber C, Weaver S, Kitron U, Reis M, Ribeiro G. Detection of Chikungunya Virus RNA in Oral Fluid and Urine: An Alternative Approach to Diagnosis? Viruses 2024, 16: 235. PMID: 38400011, PMCID: PMC10891727, DOI: 10.3390/v16020235.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, ViralChikungunya FeverChikungunya virusDengueDisease ProgressionHumansImmunoglobulin MRNA, ViralConceptsChikungunya virus infectionAcute-phase seraOral fluidChikungunya virusIgM seroconversionChikungunya virus diagnosisUrine samplesAcute febrile illnessConvalescent-phase seraAcute chikungunyaChikungunya patientsChikungunya virus RNADisease courseFebrile illnessPaired seraRT-qPCRAcute phaseIgM detectionConvalescent-phasePatientsControl group samplesSerumUrineCHIKV transmissionIgM
2023
Dynamics of chikungunya virus transmission in the first year after its introduction in Brazil: A cohort study in an urban community
Anjos R, Portilho M, Jacob-Nascimento L, Carvalho C, Moreira P, Sacramento G, Nery N, de Oliveira D, Cruz J, Cardoso C, Argibay H, Plante K, Plante J, Weaver S, Kitron U, Reis M, Ko A, Costa F, Ribeiro G. Dynamics of chikungunya virus transmission in the first year after its introduction in Brazil: A cohort study in an urban community. PLOS Neglected Tropical Diseases 2023, 17: e0011863. PMID: 38150470, PMCID: PMC10775974, DOI: 10.1371/journal.pntd.0011863.Peer-Reviewed Original Research
2022
Plasmodium infection is associated with cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein
Lapidus S, Liu F, Casanovas-Massana A, Dai Y, Huck J, Lucas C, Klein J, Filler R, Strine M, Sy M, Deme A, Badiane A, Dieye B, Ndiaye I, Diedhiou Y, Mbaye A, Diagne C, Vigan-Womas I, Mbengue A, Sadio B, Diagne M, Moore A, Mangou K, Diallo F, Sene S, Pouye M, Faye R, Diouf B, Nery N, Costa F, Reis M, Muenker M, Hodson D, Mbarga Y, Katz B, Andrews J, Campbell M, Srivathsan A, Kamath K, Baum-Jones E, Faye O, Sall A, Vélez J, Cappello M, Wilson M, Ben-Mamoun C, Tedder R, McClure M, Cherepanov P, Somé F, Dabiré R, Moukoko C, Ouédraogo J, Boum Y, Shon J, Ndiaye D, Wisnewski A, Parikh S, Iwasaki A, Wilen C, Ko A, Ring A, Bei A. Plasmodium infection is associated with cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein. Scientific Reports 2022, 12: 22175. PMID: 36550362, PMCID: PMC9778468, DOI: 10.1038/s41598-022-26709-7.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, ViralCOVID-19Cross ReactionsEpitopesHumansMalariaN-Acetylneuraminic AcidSARS-CoV-2Spike Glycoprotein, CoronavirusConceptsCross-reactive antibodiesSARS-CoV-2Positive SARS-CoV-2 antibody resultsPositive SARS-CoV-2 antibodiesSARS-CoV-2 reactivitySARS-CoV-2 antibodiesAcute malaria infectionSpike proteinAntibody test resultsPre-pandemic samplesMalaria-endemic countriesPopulation-level immunityMalaria-endemic regionsSpike S1 subunitNon-endemic countriesSARS-CoV-2 spike proteinSARS-CoV-2 proteinsPopulation-level exposureCOVID-19 transmissionMalaria exposureFalse-positive resultsMalaria infectionDisease burdenPlasmodium infectionAntibody resultsReliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveys
Belitardo E, Nery N, Ticona J, Portilho MM, Mello IO, Ribeiro GS, Reis MG, Costa F, Cummings DAT, Ko AI, Fofana MO. Reliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveys. Diagnostic Microbiology And Infectious Disease 2022, 104: 115807. PMID: 36162285, PMCID: PMC9428330, DOI: 10.1016/j.diagmicrobio.2022.115807.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, ViralCOVID-19HumansImmunoglobulin GReproducibility of ResultsSARS-CoV-2Seroepidemiologic StudiesConceptsSARS-CoV-2 incidenceCommunity-based cohortPopulation-level immunitySARS-CoV-2Large-scale serosurveysELISA optical densityIgG titersIgG ELISAPandemic evolvesSerologic surveyTitersOptical density valuesPopulation-level changesSerosurveyImmunitySingle dilutionSerial dilutionsNODCohortIncidenceELISASerum
2020
Transmission of Chikungunya Virus in a Brazilian Urban Slum - Volume 26, Number 7—July 2020 - Emerging Infectious Diseases journal - CDC
Anjos RO, Mugabe VA, Moreira PSS, Carvalho CX, Portilho MM, Khouri R, Sacramento GA, Nery NRR, Reis MG, Kitron UD, Ko AI, Costa F, Ribeiro GS. Transmission of Chikungunya Virus in a Brazilian Urban Slum - Volume 26, Number 7—July 2020 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2020, 26: 1364-1373. PMID: 32568045, PMCID: PMC7323528, DOI: 10.3201/eid2607.190846.Peer-Reviewed Original ResearchConceptsEpisodes of feverChikungunya outbreakInfectious Diseases journal - CDCSymptomatic infection ratePresumptive clinical diagnosisSufficient herd immunityCommunity-based studyFurther longitudinal studiesCHIKV seroprevalenceSeropositive personsVirus seroprevalenceClinical dataHerd immunityChikungunya virusInfection rateClinical diagnosisSerum samplesDisease pointUnpaved streetsArthralgiaLongitudinal studyFeverSeroprevalenceEpisodesOutbreak
2019
Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
Kikuti M, Tauro L, Moreira P, Nascimento L, Portilho M, Soares G, Weaver S, Reis M, Kitron U, Ribeiro G. Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. International Journal Of Infectious Diseases 2019, 91: 38-43. PMID: 31704226, DOI: 10.1016/j.ijid.2019.11.001.Peer-Reviewed Original ResearchConceptsConvalescent samplesIgM enzymeRT-PCRZika virus transmissionNegative patientsCHIKV infectionAcute samplesZIKV casesConvalescent seraSurveillance studyCHIKV transmissionZika virusCHIKV casesDiagnostic performanceLow specificityVirus transmissionEquivocal resultsTest sensitivitySerumELISA specificityPatientsHigh rateDengueConcomitant transmissionMore equivocal resultsAccuracy of the SD BIOLINE Dengue Duo for rapid point-of-care diagnosis of dengue
Kikuti M, Cruz J, Rodrigues M, Tavares A, Paploski I, Silva M, Santana P, Tauro L, Silva G, Campos G, Araújo J, Kitron U, Reis M, Ribeiro G. Accuracy of the SD BIOLINE Dengue Duo for rapid point-of-care diagnosis of dengue. PLOS ONE 2019, 14: e0213301. PMID: 30840708, PMCID: PMC6402663, DOI: 10.1371/journal.pone.0213301.Peer-Reviewed Original ResearchConceptsRapid diagnosis testsNon-dengue febrile patientsFebrile patientsHealthy controlsBlood donorsLaboratory-confirmed dengue casesSD BIOLINE Dengue DuoSecondary DENV infectionDengue virus antigenAcute-phase seraDengue diagnostic testsRepresentative serum samplesDengue-endemic regionsDengue-endemic areasSample of casesDENV antibodiesDENV infectionNS1 ELISAPrimary infectionDengue diagnosisMedical treatment decisionsTreatment decisionsVirus antigenRDT accuracyRDT sensitivity