2003
Nutrient-dependent and Insulin-stimulated Phosphorylation of Insulin Receptor Substrate-1 on Serine 302 Correlates with Increased Insulin Signaling*
Giraud J, Leshan R, Lee Y, White M. Nutrient-dependent and Insulin-stimulated Phosphorylation of Insulin Receptor Substrate-1 on Serine 302 Correlates with Increased Insulin Signaling*. Journal Of Biological Chemistry 2003, 279: 3447-3454. PMID: 14623899, DOI: 10.1074/jbc.m308631200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino AcidsAndrostadienesAnimalsBlotting, WesternBromodeoxyuridineCell DivisionCell LineCHO CellsCricetinaeCulture Media, Serum-FreeDose-Response Relationship, DrugEnzyme InhibitorsGlucoseGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaInsulinInsulin Receptor Substrate ProteinsJNK Mitogen-Activated Protein KinasesMiceMitogen-Activated Protein KinasesMolecular Sequence DataMutagenesis, Site-DirectedMutationPhosphoproteinsPhosphorylationPoint MutationPrecipitin TestsRatsSerineSignal TransductionSirolimusTime FactorsWortmanninConceptsInsulin/IGFIRS-1Insulin-stimulated signal transductionInsulin receptor substrate IRS-1Ser/Thr phosphorylationSequence-specific polyclonal antibodiesInsulin-stimulated tyrosine phosphorylationInsulin receptor substrate-1Synthase kinase-3beta phosphorylationSubstrate IRS-1IRS-1-mediated signalingRibosomal S6 proteinC-Jun kinaseInsulin-stimulated phosphorylationReceptor substrate-1IGF-I stimulationThr phosphorylationKinase associatesP85 bindingPhosphorylated residuesSignal transductionInsulin-stimulated AktTyrosine phosphorylationS6 proteinNutrient availability
1999
Early biochemical events in insulin-stimulated fluid phase endocytosis
Pitterle D, Sperling R, Myers M, White M, Blackshear P. Early biochemical events in insulin-stimulated fluid phase endocytosis. American Journal Of Physiology 1999, 276: e94-e105. PMID: 9886955, DOI: 10.1152/ajpendo.1999.276.1.e94.Peer-Reviewed Original ResearchConceptsInsulin receptor substrate-1Fluid-phase endocytosisMitogen-activated protein kinase kinaseRat-1 cellsInsulin receptorDominant negative mutant RasHematopoietic precursor cell lineBat cellsHIRc-B cellsInhibitor of PIProtein kinase kinaseWild-type RasMEK inhibitor PD 98059Active insulin receptorEndogenous IRS-1Receptor substrate-1Inhibitor PD 98059Certain cell typesPrecursor cell lineInitial molecular mechanismsIRS-1 expressionKinase kinaseEarly biochemical eventsMutant RasSubstrate-1
1998
Insulin Receptor Substrate-1 is the Predominant Signaling Molecule Activated by Insulin-like Growth Factor-I, Insulin, and Interleukin-4 in Estrogen Receptor-positive Human Breast Cancer Cells*
Jackson J, White M, Yee D. Insulin Receptor Substrate-1 is the Predominant Signaling Molecule Activated by Insulin-like Growth Factor-I, Insulin, and Interleukin-4 in Estrogen Receptor-positive Human Breast Cancer Cells*. Journal Of Biological Chemistry 1998, 273: 9994-10003. PMID: 9545345, DOI: 10.1074/jbc.273.16.9994.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesBreast NeoplasmsCalcium-Calmodulin-Dependent Protein KinasesEnzyme InhibitorsFemaleFlavonoidsHumansInsulinInsulin Receptor Substrate ProteinsInsulin-Like Growth Factor IInterleukin-4Intracellular Signaling Peptides and ProteinsKineticsMitogen-Activated Protein Kinase KinasesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphoproteinsPhosphorylationPhosphotyrosineProtein Kinase InhibitorsProtein KinasesReceptor, InsulinReceptors, EstrogenSignal TransductionTumor Cells, CulturedWortmanninConceptsIRS-1Tyrosine phosphorylationIRS-2Insulin-like growth factorBreast cancer cellsIGF-I treatmentGreater tyrosine phosphorylationInterleukin-4Substrate adaptor proteinMitogen-activated protein kinase activityCancer cellsCell linesInsulin receptor substrate-1Mitogen-activated protein kinaseP85 regulatory subunitProtein kinase activityActivation of phosphatidylinositolReceptor substrate-1Estrogen receptor-positive human breast cancer cellsGrowth factorPrimary breast tumor specimensIGF-stimulated growthAdaptor proteinRegulatory subunitT47-D breast cancer cells
1997
Activation of the phosphatidylinositol 3-kinase serine kinase by IFN-alpha.
Uddin S, Fish E, Sher D, Gardziola C, White M, Platanias L. Activation of the phosphatidylinositol 3-kinase serine kinase by IFN-alpha. The Journal Of Immunology 1997, 158: 2390-7. PMID: 9036989, DOI: 10.4049/jimmunol.158.5.2390.Peer-Reviewed Original ResearchConceptsSerine kinaseTreatment of cellsIRS-1Kinase assaysSerine kinase activityDual-specificity enzymeP85 regulatory subunitReceptor-generated signalsIRS-1 proteinJak-1 kinasesIFN-alpha-induced activationProtein associatesP85 subunitPhosphoaminoacid analysisRegulatory subunitSerine residuesSerine phosphorylationTyrosine phosphorylationTyk-2STAT-2MAP kinaseKinase activityPretreatment of cellsInhibitor wortmanninPhosphatidylinositol