Interaction of insulin receptor substrate-1 (IRS-1) with phosphatidylinositol 3-kinase: effect of substitution of serine for alanine in potential IRS-1 serine phosphorylation sites.
Delahaye L, Mothe-Satney I, Myers M, White M, Van Obberghen E. Interaction of insulin receptor substrate-1 (IRS-1) with phosphatidylinositol 3-kinase: effect of substitution of serine for alanine in potential IRS-1 serine phosphorylation sites. Endocrinology 1998, 139: 4911-9. PMID: 9832428, DOI: 10.1210/endo.139.12.6379.Peer-Reviewed Original ResearchConceptsInsulin receptor substrate-1Protein kinase B activitySerine phosphorylation sitesRegulatory subunitReceptor substrate-1Phosphorylation sitesPotential binding sitesTyrosine phosphorylationSubstrate-1Potential tyrosine phosphorylation sitesIRS-1 interactsPotential serine phosphorylation sitesWild-type IRS-1Two-hybrid systemTyrosine phosphorylation sitesInsulin-stimulated phosphatidylinositolPhosphorylate IRS-1P85alpha regulatory subunitBinding sitesYeast kinasesThreonine phosphorylationSerine mutantsYXXM motifsB activityP85alpha