2001
PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells
Fleischhauer J, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2001, 281: c1614-c1623. PMID: 11600425, DOI: 10.1152/ajpcell.2001.281.5.c1614.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsCiliary epithelial cellsCl- channelsEpithelial cellsPE cellsCl- channel activityAction of nucleotidesProtein kinaseNonpigmented ciliary epithelial cellsSecond messengerCell sortingChannel activityATP activationElectronic cell sortingATPSequential cascadeChannel activationNucleotidesP2Y receptorsPatch clampingATP assayATP releaseCellsMicroM ATPCAMP
1995
Immortalization of cat iris sphincter smooth muscle cells by SV40 virus: Growth, morphological, biochemical and pharmacological characteristics
Ocklind A, Yousufzai S, Ghosh S, Coca-Prados M, St Jernschantz J, Abdel-Latif A. Immortalization of cat iris sphincter smooth muscle cells by SV40 virus: Growth, morphological, biochemical and pharmacological characteristics. Experimental Eye Research 1995, 61: 535-545. PMID: 8654496, DOI: 10.1016/s0014-4835(05)80047-8.Peer-Reviewed Original ResearchConceptsSignal transduction pathwaysCat iris sphincter smooth muscle cellsSmooth muscle alpha-actinMuscle alpha-actinImmortalized cellsTransduction pathwaysSmooth muscle cellsMuscle cellsAlpha-actinImportant model systemSV40 virusLarge T antigenImmortalized cell culturesMlc proteinNormal muscle cellsTransformation phenotypeSuccessful immortalizationLow-serum mediumCellular DNAContact inhibitionPopulation doublingsSV-CISM-2 cellsT antigenViral transformationArachidonic acid
1994
EP2-receptor Stimulated Cyclic Amp Synthesis in Cultured Human Non-pigmented Ciliary Epithelium
Jumblatt M, Neltner A, Coca-Prados M, Paterson C. EP2-receptor Stimulated Cyclic Amp Synthesis in Cultured Human Non-pigmented Ciliary Epithelium. Experimental Eye Research 1994, 58: 563-566. PMID: 7925693, DOI: 10.1006/exer.1994.1050.Peer-Reviewed Original ResearchConceptsHuman NPE cellsReceptor subtypesProstaglandin E2 receptor subtypesCiliary epitheliumCAMP synthesisNon-pigmented epithelial cellsNPE cellsEP2 receptor subtypeCyclic AMP synthesisNon-pigmented ciliary epitheliumHuman non-pigmented ciliary epitheliumEP2 subtypeProstanoid agonistsPharmacologic characterizationSpecific receptorsHuman NonPhysiologic functionProstanoidsEpithelial cellsMethyl xanthineSubtypesAMP synthesisCyclic AMPCyclic monophosphatePGE2