2018
The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells
Rodríguez-Menéndez S, García M, Fernández B, Álvarez L, Fernández-Vega-Cueto A, Coca-Prados M, Pereiro R, González-Iglesias H. The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells. Nutrients 2018, 10: 1874. PMID: 30513827, PMCID: PMC6315569, DOI: 10.3390/nu10121874.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumOxidative stressRetinal pigment epithelial cellsOxidative damagePre-treated cellsInduces oxidative stressPigment epithelial cellsNon-treated cellsReactive oxygen intermediatesΜM of zincPigment epitheliumRPE cellsZn-MTProtective mechanismEpithelial cellsFree radical generatorMT levelsAAPH treatmentOxygen intermediates
2017
Quantitative study of zinc and metallothioneins in the human retina and RPE cells by mass spectrometry-based methodologies
Rodríguez-Menéndez S, Fernández B, García M, Álvarez L, Fernández M, Sanz-Medel A, Coca-Prados M, Pereiro R, González-Iglesias H. Quantitative study of zinc and metallothioneins in the human retina and RPE cells by mass spectrometry-based methodologies. Talanta 2017, 178: 222-230. PMID: 29136815, DOI: 10.1016/j.talanta.2017.09.024.Peer-Reviewed Original Research
2016
The zinc‐metallothionein redox system in human retina and RPE
Alvarez L, García M, Rodríguez S, Fernández B, Pereiro R, Sanz‐Medel A, Coca‐Prados M, González‐Iglesias H. The zinc‐metallothionein redox system in human retina and RPE. Acta Ophthalmologica 2016, 94 DOI: 10.1111/j.1755-3768.2016.0560.Peer-Reviewed Original ResearchHuman RPE cellsInflammatory cytokinesNeuroprotective functionOxidative stress processesEye sectionsNeural retinaHuman donorsRPE cellsRetinaHuman retinaHuman eyeRPEMT proteinExogenous zincCellular zinc homeostasisCellular modelZinc homeostasisMain regulatorStoichiometric transitionsEyesBinding profileProtein binding profileProtein synthesisLesser levelsMetallothionein
2003
CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells
Kennedy BN, Li C, Ortego J, Coca-Prados M, Sarthy VP, Crabb JW. CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells. Experimental Eye Research 2003, 76: 257-260. PMID: 12565814, DOI: 10.1016/s0014-4835(02)00308-1.Peer-Reviewed Original ResearchConceptsTranscriptional regulationBinding Protein FunctionsCell-specific expressionPromoter analysisProtein functionRepressor elementEnhancer elementsPromoter constructsReporter activityRetinal pigment epithelial cellsPigment epithelial cellsCiliary epitheliumEpithelial cellsVisual cycleRPE cellsBPRegulationRetinal pigment epitheliumCellsMüller cellsGenesMutationsRLBP1 geneEpitheliumWildtype