2023
Acetate controls endothelial-to-mesenchymal transition
Zhu X, Wang Y, Soaita I, Lee H, Bae H, Boutagy N, Bostwick A, Zhang R, Bowman C, Xu Y, Trefely S, Chen Y, Qin L, Sessa W, Tellides G, Jang C, Snyder N, Yu L, Arany Z, Simons M. Acetate controls endothelial-to-mesenchymal transition. Cell Metabolism 2023, 35: 1163-1178.e10. PMID: 37327791, PMCID: PMC10529701, DOI: 10.1016/j.cmet.2023.05.010.Peer-Reviewed Original ResearchConceptsTGF-β signalingChronic vascular diseaseTGF-β receptor ALK5Mesenchymal transitionInduction of EndMTVascular diseaseMolecular basisPositive feedback loopReceptor ALK5Cellular levelSMADs 2Novel targetEndMT inductionMetabolic modulationMetabolic basisFibrotic stateSignalingPotential treatmentEndMTTGFDiseaseActivationInductionACSS2PDK4
2013
Endothelial ERK signaling controls lymphatic fate specification
Deng Y, Atri D, Eichmann A, Simons M. Endothelial ERK signaling controls lymphatic fate specification. Journal Of Clinical Investigation 2013, 123: 1202-1215. PMID: 23391722, PMCID: PMC3582116, DOI: 10.1172/jci63034.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaBody PatterningButadienesCells, CulturedEndothelium, LymphaticExtracellular Signal-Regulated MAP KinasesFemaleGene ExpressionGene Expression Regulation, DevelopmentalHomeodomain ProteinsHuman Umbilical Vein Endothelial CellsHumansLymphangiectasisLymphangiogenesisMaleMAP Kinase Signaling SystemMiceMice, TransgenicMutation, MissenseNitrilesProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-rafSOXF Transcription FactorsTumor Suppressor ProteinsUp-RegulationVascular Endothelial Growth Factor Receptor-3ConceptsFate specificationERK activationSOX18 expressionEndothelial cellsLymphatic endothelial cellsInhibition of ERKLymphatic fateDifferentiation programNoonan syndromeLymphatic phenotypeInducible expressionRAF1 geneMolecular eventsFunction mutationsProx1 expressionVenous endothelial cellsCardinal veinERKExpressionLymphatic vesselsKey roleRelated diseasesSOX18ActivationExcessive production
2012
FGF Regulates TGF-β Signaling and Endothelial-to-Mesenchymal Transition via Control of let-7 miRNA Expression
Chen PY, Qin L, Barnes C, Charisse K, Yi T, Zhang X, Ali R, Medina PP, Yu J, Slack FJ, Anderson DG, Kotelianski V, Wang F, Tellides G, Simons M. FGF Regulates TGF-β Signaling and Endothelial-to-Mesenchymal Transition via Control of let-7 miRNA Expression. Cell Reports 2012, 2: 1684-1696. PMID: 23200853, PMCID: PMC3534912, DOI: 10.1016/j.celrep.2012.10.021.Peer-Reviewed Original ResearchConceptsFibroblast growth factorEndo-MTMesenchymal transitionGrowth factorNormal endothelial functionBlood vessel functionTGF-β signalingEndothelial functionVascular pathologyEndothelial homeostasisNeointima formationVessel functionΒ ligandMiRNA levelsMiRNA expressionActivationExpressionUnexpected role
2008
Arterial branching morphogenesis
Simons M. Arterial branching morphogenesis. The FASEB Journal 2008, 22: 520.1-520.1. DOI: 10.1096/fasebj.22.1_supplement.520.1.Peer-Reviewed Original ResearchVariety of signalsArterial morphogenesisArterial differentiationBranching morphogenesisEmbryonic developmentZebrafish modelPDGF stimulationOrgan growthAdult tissuesGene expressionIntracellular signalingMorphogenesisCell typesMolecular defectsTree sizeKey eventsEndothelial cellsRegulationAbnormal activationKey roleObvious importanceActivationSynectinRac1Signaling
2000
Thrombospondin Type 1 Repeats Interact with Matrix Metalloproteinase 2 REGULATION OF METALLOPROTEINASE ACTIVITY*
Bein K, Simons M. Thrombospondin Type 1 Repeats Interact with Matrix Metalloproteinase 2 REGULATION OF METALLOPROTEINASE ACTIVITY*. Journal Of Biological Chemistry 2000, 275: 32167-32173. PMID: 10900205, DOI: 10.1074/jbc.m003834200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesBlotting, WesternCatalytic DomainCattleCell LineCollagenasesEnzyme ActivationEnzyme PrecursorsHumansMatrix Metalloproteinase 2Matrix Metalloproteinase 3Matrix Metalloproteinase 9Matrix Metalloproteinase InhibitorsPeptide FragmentsPrecipitin TestsProperdinProtein BindingProtein Structure, TertiaryRepetitive Sequences, Amino AcidSaccharomyces cerevisiaeThrombinThrombospondin 1ThrombospondinsTwo-Hybrid System TechniquesConceptsIntact thrombospondin-1Thrombospondin-1Ability of TSP1Matrix metalloproteinase-2Two-hybrid systemType 1 repeatsCatalytic domainCDNA clonesProtein interactionsThrombospondin type 1Terminal regionGelatin-binding domainAmino acidsInhibitors of angiogenesisCatalytic centerMMP activityTSP2Western blottingProteinTissue cultureClonesMapping sitesMetalloproteinase activityActivationMetalloproteinase-2
1999
Phosphatidylinositol-4,5-bisphosphate Mediates the Interaction of Syndecan-4 with Protein Kinase C †
Horowitz A, Murakami M, Gao Y, Simons M. Phosphatidylinositol-4,5-bisphosphate Mediates the Interaction of Syndecan-4 with Protein Kinase C †. Biochemistry 1999, 38: 15871-15877. PMID: 10625452, DOI: 10.1021/bi991363i.Peer-Reviewed Original ResearchConceptsCytoplasmic tailSyndecan-4Protein kinase CalphaProtein kinase CExtracellular matrix proteinsActivation of PKCalphaGrowth factorExtracellular signalsDelta isoenzymesTransmembrane proteoglycansKinase CMatrix proteinsPhosphatidylinositolPKCalphaActivation mechanismCooperative activationBisphosphateActivationProteoglycansRecent studiesTailPICalphaSignalingAffinity