2021
The neural G protein Gαo tagged with GFP at an internal loop is functional in Caenorhabditis elegans
Kumar S, Olson AC, Koelle MR. The neural G protein Gαo tagged with GFP at an internal loop is functional in Caenorhabditis elegans. G3: Genes, Genomes, Genetics 2021, 11: jkab167. PMID: 34003969, PMCID: PMC8496287, DOI: 10.1093/g3journal/jkab167.Peer-Reviewed Original ResearchConceptsGreen fluorescent proteinCaenorhabditis elegansGenetic analysisHeterotrimeric G proteinsG protein GαoInternal loopC. elegansProtein complexesBiochemical purificationEpitope tagPlasma membraneAlpha subunitMolecular mechanismsFluorescent proteinGenetic studiesElegansGαoG proteinsEgg layingTransgenic expressionBiochemical studiesGαo proteinsBody morphologyProteinNeurotransmitter release
2009
RSBP-1 Is a Membrane-targeting Subunit Required by the Gαq-specific But Not the Gαo-specific R7 Regulator of G protein Signaling in Caenorhabditis elegans
Porter MY, Koelle MR. RSBP-1 Is a Membrane-targeting Subunit Required by the Gαq-specific But Not the Gαo-specific R7 Regulator of G protein Signaling in Caenorhabditis elegans. Molecular Biology Of The Cell 2009, 21: 232-243. PMID: 19923320, PMCID: PMC2808233, DOI: 10.1091/mbc.e09-07-0642.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsCell MembraneGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11GTP-Binding Protein RegulatorsImmunoprecipitationLocomotionMembrane ProteinsMolecular Sequence DataMusclesMutationNervous SystemOvulationProtein TransportRGS ProteinsSequence AlignmentSequence Homology, Amino AcidSignal TransductionSubcellular FractionsTransgenesConceptsR7 RGS proteinsRGS proteinsCaenorhabditis elegansEGL-10EAT-16G protein signaling (RGS) proteinsG proteinsMembrane-targeting sequenceGalpha GTPase activityC. elegans neuronsPhenocopies lossR7 regulatorMembrane associationRGS activityMembrane localizationProtein familyR7 familySignaling proteinsGTPase activityPlasma membraneGenetic studiesCultured cellsProteinR7BPElegans
2006
Heterotrimeric G Protein Signaling: Getting inside the Cell
Koelle MR. Heterotrimeric G Protein Signaling: Getting inside the Cell. Cell 2006, 126: 25-27. PMID: 16839871, DOI: 10.1016/j.cell.2006.06.026.Peer-Reviewed Original Research