2019
Serotonin and neuropeptides are both released by the HSN command neuron to initiate C. elegans egg laying
Brewer JC, Olson AC, Collins KM, Koelle MR. Serotonin and neuropeptides are both released by the HSN command neuron to initiate C. elegans egg laying. PLOS Genetics 2019, 15: e1007896. PMID: 30677018, PMCID: PMC6363226, DOI: 10.1371/journal.pgen.1007896.Peer-Reviewed Original ResearchConceptsHermaphrodite-specific neuronsEgg-laying defectsNLP-3C. elegansEgg-laying musclesEgg-laying circuitDirect postsynaptic targetsEgg-laying behaviorSerotonergic Hermaphrodite Specific NeuronsMuscle cellsSmall molecule neurotransmittersNull mutantsHSN neuronsDouble mutantSingle mutantsMutant animalsSerotonergic neuronsWild-type animalsSevere defectsMutantsElegansNeuropeptide substance PMammalian brainEggsSpecific neurons
2013
Postsynaptic ERG Potassium Channels Limit Muscle Excitability to Allow Distinct Egg-Laying Behavior States in Caenorhabditis elegans
Collins KM, Koelle MR. Postsynaptic ERG Potassium Channels Limit Muscle Excitability to Allow Distinct Egg-Laying Behavior States in Caenorhabditis elegans. Journal Of Neuroscience 2013, 33: 761-775. PMID: 23303953, PMCID: PMC3542984, DOI: 10.1523/jneurosci.3896-12.2013.Peer-Reviewed Original ResearchConceptsEgg-laying musclesBody bendsUNC-103Caenorhabditis elegansCalcium transientsTwo-state behaviorERG potassium channelsMutantsERG channelsPotassium channelsInactive phaseEggsDistinct behavioral statesMuscle excitabilityPostsynaptic excitabilityPostsynaptic sitesERG functionCaenorhabditisCalcium imagingElegansFurther adjustmentExcitabilityRhythmic excitationMuscleBehavioral states
2011
Two types of chloride transporters are required for GABAA receptor‐mediated inhibition in C. elegans
Bellemer A, Hirata T, Romero MF, Koelle MR. Two types of chloride transporters are required for GABAA receptor‐mediated inhibition in C. elegans. The EMBO Journal 2011, 30: 1852-1863. PMID: 21427702, PMCID: PMC3101993, DOI: 10.1038/emboj.2011.83.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedAnion Transport ProteinsBrainCaenorhabditis elegansCaenorhabditis elegans ProteinsChloridesElectrophysiologyGene Expression RegulationHydrogen-Ion ConcentrationMicroscopyMotor ActivityMutationNeuronsOocytesPlasmidsReceptors, GABA-ASymportersTransgenesXenopusConceptsCaenorhabditis elegans mutantC. elegansSynapse developmentInhibits cellBehavioral defectsCl- gradientGABAA receptor-mediated inhibitionMutantsReceptor-mediated inhibitionTransportersChloride transportersCl- channelsIdentified mutationsNeuronal expressionCl(-) cotransporterCl(-) extruderInhibitory neurotransmissionChloride gradientChloride influxElegansCellsSevere disruptionCL flowNeural activityPrincipal mechanism
2004
Mechanism of extrasynaptic dopamine signaling in Caenorhabditis elegans
Chase DL, Pepper JS, Koelle MR. Mechanism of extrasynaptic dopamine signaling in Caenorhabditis elegans. Nature Neuroscience 2004, 7: 1096-1103. PMID: 15378064, DOI: 10.1038/nn1316.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsDNA, ComplementaryDopamineGene TargetingGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11GTP-Binding ProteinsMolecular Sequence DataMotor ActivityMotor NeuronsMutationNervous SystemPhylogenyReceptors, DopamineReceptors, Dopamine D1Receptors, Dopamine D2RGS ProteinsSequence Homology, Amino AcidSequence Homology, Nucleic AcidSignal TransductionConceptsCaenorhabditis elegansDOP-3DOP-1D2-like receptorsSignaling ComplexC. elegans locomotionLocomotion defectsExtrasynaptic dopamineAntagonistic effectGαoGαqElegansD2-like dopamine receptorsD1-like receptorsSame motor neuronsPathwayReceptorsDopaminergic neuronsDopamine receptorsMotor neuronsMutantsGenesDopamineSubunitsRegulatorGenetic Analysis of RGS Protein Function in Caenorhabditis elegans
Chase DL, Koelle MR. Genetic Analysis of RGS Protein Function in Caenorhabditis elegans. Methods In Enzymology 2004, 389: 305-320. PMID: 15313573, DOI: 10.1016/s0076-6879(04)89018-9.Peer-Reviewed Original ResearchConceptsRGS proteinsC. elegansG proteinsRGS protein functionStructure/function studiesG protein geneCaenorhabditis elegansGalpha mutantsClose homologProtein functionGalpha proteinsElegansGenetic analysisDetailed protocolTransgenic expressionProteinMost mammaliansMutantsFunction studiesOrthologsCaenorhabditisHomologMammalianGenesOrganisms
2003
Genetic and Cellular Basis for Acetylcholine Inhibition of Caenorhabditis elegans Egg-Laying Behavior
Bany IA, Dong MQ, Koelle MR. Genetic and Cellular Basis for Acetylcholine Inhibition of Caenorhabditis elegans Egg-Laying Behavior. Journal Of Neuroscience 2003, 23: 8060-8069. PMID: 12954868, PMCID: PMC6740490, DOI: 10.1523/jneurosci.23-22-08060.2003.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAcetylcholinesteraseAnimalsAnimals, Genetically ModifiedBehavior, AnimalCaenorhabditis elegansCaenorhabditis elegans ProteinsCholinesterase InhibitorsDNA, ComplementaryGTP-Binding ProteinsHomeodomain ProteinsInhibition, PsychologicalMutationNeuronsNuclear ProteinsOvipositionPhenotypeReceptors, CholinergicSignal TransductionSynapsesConceptsHermaphrodite-specific neuronsEgg-laying behaviorG proteinsG-protein signaling genesEgg-laying defectsEgg-laying musclesEgg-laying systemAnalysis of mutantsInhibition of eggSerotonergic Hermaphrodite Specific NeuronsUnc-4Caenorhabditis elegansUnc-17Signaling GenesThird cell typeActivation of eggsMorphological defectsCha-1MutantsCell typesCellular basisNeurotransmitter releaseGenesEggsPartial defect
2001
Two RGS proteins that inhibit Gαo and Gαq signaling in C. elegans neurons require a Gβ5-like subunit for function
Chase D, Patikoglou G, Koelle M. Two RGS proteins that inhibit Gαo and Gαq signaling in C. elegans neurons require a Gβ5-like subunit for function. Current Biology 2001, 11: 222-231. PMID: 11250150, DOI: 10.1016/s0960-9822(01)00071-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedBehavior, AnimalCaenorhabditis elegansCaenorhabditis elegans ProteinsGene ExpressionGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11GTP-Binding Protein beta SubunitsGTP-Binding Protein RegulatorsGTP-Binding ProteinsHelminth ProteinsHeterotrimeric GTP-Binding ProteinsMiceNeuronsOvipositionRGS ProteinsSignal TransductionTransgenesConceptsRGS proteinsEGL-10EAT-16C. elegansG protein signaling (RGS) proteinsG protein heterotrimersC. elegans neuronsG protein signalingGPB-2Gbeta proteinsGTPase activatorNull mutantsRGS activityDouble mutantSignaling proteinsProtein signalingG proteinsPhysiological roleMutantsProteinKnockout animalsElegansObvious defectsRGSSubunits
1999
Antagonism between Goα and Gqα in Caenorhabditis elegans: the RGS protein EAT-16 is necessary for Goα signaling and regulates Gqα activity
Hajdu-Cronin Y, Chen W, Patikoglou G, Koelle M, Sternberg P. Antagonism between Goα and Gqα in Caenorhabditis elegans: the RGS protein EAT-16 is necessary for Goα signaling and regulates Gqα activity. Genes & Development 1999, 13: 1780-1793. PMID: 10421631, PMCID: PMC316886, DOI: 10.1101/gad.13.14.1780.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCaenorhabditis elegansCaenorhabditis elegans ProteinsCOS CellsDNA PrimersGene Expression RegulationGenes, SuppressorGTP-Binding Protein RegulatorsGTP-Binding ProteinsHelminth ProteinsMolecular Sequence DataMutationSequence Homology, Amino AcidSignal TransductionConceptsEGL-30Cellular rolesEAT-16Double mutant analysisMajor cellular roleHeterotrimeric G proteinsG protein signalingMolecular genetic approachesCOS-7 cellsGOA-1Function mutantsCaenorhabditis elegansC. elegansDouble mutantProtein signalingGenetic approachesG proteinsSAG-1ElegansMutantsGenesGoαHyperactive phenotypeProteinMutations