2019
Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells.
Ventura A, Vassall A, Yurter A, Robinson E, Filler R, Hanlon D, Meeth K, Ezaldein H, Girardi M, Sobolev O, Bosenberg MW, Edelson RL. Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells. Journal Of Visualized Experiments 2019 PMID: 31157760, DOI: 10.3791/59370.Peer-Reviewed Original ResearchConceptsCutaneous T-cell lymphomaDendritic cellsCellular vaccinesClinical efficacyAnti-tumor T cell immunityVivo anti-tumor responsesMonocyte-derived dendritic cellsTumor cellsSyngeneic mouse tumor modelsImmunogenic dendritic cellsAnti-cancer immunityT cell immunityAnti-tumor responseHuman dendritic cellsT-cell lymphomaAnti-tumor effectsKey mechanistic driversApoptotic tumor cellsMouse tumor modelsCell immunitySafety profileCancer immunotherapyCell lymphomaMouse modelBlood samples
2018
Extracorporeal Photochemotherapy Drives Monocyte-to-Dendritic Cell Maturation to Induce Anti-Cancer Immunity
Ventura A, Vassall A, Robinson E, Filler R, Hanlon D, Meeth K, Ezaldein H, Girardi M, Sobolev O, Bosenberg MW, Edelson RL. Extracorporeal Photochemotherapy Drives Monocyte-to-Dendritic Cell Maturation to Induce Anti-Cancer Immunity. Cancer Research 2018, 78: canres.0171.2018. PMID: 29764863, DOI: 10.1158/0008-5472.can-18-0171.Peer-Reviewed Original ResearchConceptsT cellsT cell antitumor immunityTumor-specific T cellsTumor cellsEffective immunotherapeutic agentFavorable safety profileResponder T cellsDendritic cell differentiationTumor-challenged miceImmunogenic cell deathSelective antitumor effectApoptotic tumor cellsPotential therapeutic applicabilityProcessing/presentationAntimelanoma immunityHealthy DCsImmunogenic malignanciesAntitumor immunityCellular vaccinesImmunotherapeutic effectsAdditional malignanciesImmunotherapeutic agentsSafety profileCancer immunotherapyTumor antigens
2010
Rapid generation of maturationally synchronized human dendritic cells: contribution to the clinical efficacy of extracorporeal photochemotherapy
Berger C, Hoffmann K, Vasquez JG, Mane S, Lewis J, Filler R, Lin A, Zhao H, Durazzo T, Baird A, Lin W, Foss F, Christensen I, Girardi M, Tigelaar R, Edelson R. Rapid generation of maturationally synchronized human dendritic cells: contribution to the clinical efficacy of extracorporeal photochemotherapy. Blood 2010, 116: 4838-4847. PMID: 20720185, PMCID: PMC3321745, DOI: 10.1182/blood-2009-11-256040.Peer-Reviewed Original ResearchMeSH KeywordsAntigen PresentationCell DifferentiationCell SeparationDendritic CellsFlow CytometryGene ExpressionGraft vs Host DiseaseHumansImmunophenotypingIn Situ HybridizationLymphoma, T-Cell, CutaneousMonocytesOligonucleotide Array Sequence AnalysisPhotopheresisReverse Transcriptase Polymerase Chain ReactionConceptsCutaneous T-cell lymphomaCostimulatory surface moleculesHuman dendritic cellsT-cell lymphomaDC differentiation pathwayAutoreactive disordersTherapeutic DCsDifferentiation pathwayHost diseaseDendritic cellsExtracorporeal photochemotherapyDistinctive molecular signaturesClinical efficacyOrgan rejectionCancer immunotherapyFunctional DCsEndogenous antigensNormal monocytesNormal subjectsClinical successCell differentiation pathwayLevel of expressionDisease statesMonocytesEnhanced expression
2009
Molecular Analysis of Tumor-Promoting CD8+ T Cells in Two-Stage Cutaneous Chemical Carcinogenesis
Kwong BY, Roberts SJ, Silberzahn T, Filler RB, Neustadter JH, Galan A, Reddy S, Lin WM, Ellis PD, Langford CF, Hayday AC, Girardi M. Molecular Analysis of Tumor-Promoting CD8+ T Cells in Two-Stage Cutaneous Chemical Carcinogenesis. Journal Of Investigative Dermatology 2009, 130: 1726-1736. PMID: 19924136, PMCID: PMC2920801, DOI: 10.1038/jid.2009.362.Peer-Reviewed Original ResearchMeSH Keywords9,10-Dimethyl-1,2-benzanthraceneAmphiregulinAnimalsCD8-Positive T-LymphocytesCell DifferentiationDisease Models, AnimalEGF Family of ProteinsForkhead Transcription FactorsGene Expression ProfilingGlycoproteinsHepatitis A Virus Cellular Receptor 2Intercellular Signaling Peptides and ProteinsInterleukin-10Interleukin-17MiceMice, KnockoutReceptors, Antigen, T-Cell, alpha-betaReceptors, VirusSkin NeoplasmsConceptsTumor-infiltrating lymphocytesT cellsCutaneous carcinogenesisIL-17-producing T cellsT-proCutaneous chemical carcinogenesisTricolor flow cytometryContribution of inflammationCytolytic T cellsTumor-bearing miceEpithelial growth factorMechanism of actionT helperCancer immunotherapyUseful biomarkerMalignant progressionFlow cytometryClear associationGrowth factorReduced cytotoxicChemical carcinogenesisCarcinogenesisPro phenotypeWhole genome expression analysisTime-course analysis