Featured Publications
Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response
Bungiro RD, Harrison LM, Dondji B, Cappello M. Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response. PLOS Neglected Tropical Diseases 2022, 16: e0010098. PMID: 34986139, PMCID: PMC8765627, DOI: 10.1371/journal.pntd.0010098.Peer-Reviewed Original ResearchMeSH KeywordsAncylostomaAncylostomiasisAnimalsCricetinaeDisease Models, AnimalHemoglobinsMaleMesocricetusMouthSkinConceptsCellular immune responsesGolden Syrian hamstersImmune responsePercutaneous infectionOral infectionSyrian hamstersAncylostoma ceylanicumPrimary immune responseRoute of infectionModerate growth delayFaecal egg outputPercutaneous groupImmunological manifestationsAntibody titersHookworm Ancylostoma ceylanicumInfected hamstersWorm recoveryLarval antigensBlood hemoglobinDisease pathogenesisHost responseGrowth delayHuman infectionsInfectionA. ceylanicum
2013
Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection
Wiśniewski M, Jaros S, Bąska P, Cappello M, Wędrychowicz H. Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection. Acta Parasitologica 2013, 58: 376-383. PMID: 23990436, DOI: 10.2478/s11686-013-0151-9.Peer-Reviewed Original ResearchMeSH KeywordsAncylostomaAncylostomiasisAnimalsCricetinaeDisease Models, AnimalMaleMesocricetusMetalloproteasesVaccinationVaccines, DNAConceptsChallenge infectionAnthelminthic drug resistanceEffective human vaccineWorm burden reductionsGroups of hamstersIron deficiency anemiaCDNA vaccineTriple immunizationInfected childrenDeficiency anemiaDNA vaccineHookworm infectionDNA vaccinationHuman vaccinesAnimal modelsNumerous antigensVaccine developmentGrowth retardationIntestinal nematodesDrug resistanceVaccineBurden reductionPartial protectionInfectionHemoglobin digestion
2012
Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum
Davey D, Manickam N, Simms BT, Harrison LM, Vermeire JJ, Cappello M. Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum. Experimental Parasitology 2012, 133: 243-249. PMID: 23232252, PMCID: PMC3580025, DOI: 10.1016/j.exppara.2012.11.010.Peer-Reviewed Original ResearchConceptsExperimental infectionThird-stage hookworm larvaeParasite-specific IgAHumoral immune responseStudy of pathogenesisResource-limited countriesHuman hookworm diseaseHookworm infectionLifelong susceptibilityPrimary infectionImmune responseProlonged susceptibilitySubsequent challengeAntibody productionVaccine developmentHookworm larvaeHookworm diseaseIntestinal nematodesInfectionPathological effectsRepeated exposureAncylostoma ceylanicumContinued susceptibilityMajor causeMediate resistance
2010
Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis
Espitia CM, Zhao W, Saldarriaga O, Osorio Y, Harrison LM, Cappello M, Travi BL, Melby PC. Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis. BMC Immunology 2010, 11: 31. PMID: 20569429, PMCID: PMC2909172, DOI: 10.1186/1471-2172-11-31.Peer-Reviewed Original ResearchConceptsReal-time RT-PCR assaysReal-time reverse transcriptase PCRRT-PCR assaysReverse transcriptase-PCRCutaneous leishmaniasisType 2 cytokine responsesNew World cutaneous leishmaniasisMRNA expressionDistinct immunological featuresCytokine mRNA expressionChemokine ligand 22Analysis of interleukinChemokine receptor 4Transcriptase-PCRReal-time RT-PCRImmune response moleculesMechanisms of diseaseIL-21IL-10Cytokine responsesFoxp3 expressionIL-12p40Immunological featuresIL-13Duplex real-time RT-PCR assay
2005
Detection of excretory/secretory coproantigens in experimental hookworm infection.
Bungiro RD, Cappello M. Detection of excretory/secretory coproantigens in experimental hookworm infection. American Journal Of Tropical Medicine And Hygiene 2005, 73: 915-20. PMID: 16282303, DOI: 10.4269/ajtmh.2005.73.915.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayMolecular host-parasite interactionsFuture diagnostic methodsExcretory/secretoryFecal extractsImmunosorbent assayA. ceylanicum infectionExperimental hookworm infectionIntestinal worm burdenRabbit polyclonal IgG antibodyPolyclonal IgG antibodiesES proteinsDiagnostic methodsHookworm antigensDetectionHookworm infectionIgG antibodiesStool samplesWorm burdenDay 17Hamster serumCoproantigenFaecal eggMug/Degrees C.
2003
Oral Transfer of Adult Ancylostoma ceylanicum Hookworms into Permissive and Nonpermissive Host Species
Bungiro RD, Anderson BR, Cappello M. Oral Transfer of Adult Ancylostoma ceylanicum Hookworms into Permissive and Nonpermissive Host Species. Infection And Immunity 2003, 71: 1880-1886. PMID: 12654804, PMCID: PMC152085, DOI: 10.1128/iai.71.4.1880-1886.2003.Peer-Reviewed Original ResearchConceptsBALB/c miceHigh-dose groupHumoral immune responseCD-1 miceLong-term survivalRecovery of parasitesExcretory-secretory productsHemoglobin levelsPrechallenge levelsC57BL/6 miceHookworm infectionC miceWeanling hamstersOral infectionImmune responseMurine modelHookworm larvaeSyrian hamstersMouse strainsTypical infectionsInfectionPrior exposureMiceAdult wormsOral transferShort report: Ancylostoma ceylanicum: exsheathment is not required for successful cryopreservation of third stage hookworm larvae.
Duarte J, Harrison LM, Cappello M. Short report: Ancylostoma ceylanicum: exsheathment is not required for successful cryopreservation of third stage hookworm larvae. American Journal Of Tropical Medicine And Hygiene 2003, 68: 44-5. PMID: 12556146, DOI: 10.4269/ajtmh.2003.68.1.0680044.Peer-Reviewed Original Research