2014
Transcriptional profiling of the spleen in progressive visceral leishmaniasis reveals mixed expression of type 1 and type 2 cytokine-responsive genes
Espitia CM, Saldarriaga OA, Travi BL, Osorio EY, Hernandez A, Band M, Patel MJ, Medina AA, Cappello M, Pekosz A, Melby PC. Transcriptional profiling of the spleen in progressive visceral leishmaniasis reveals mixed expression of type 1 and type 2 cytokine-responsive genes. BMC Immunology 2014, 15: 38. PMID: 25424735, PMCID: PMC4253007, DOI: 10.1186/s12865-014-0038-z.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCluster AnalysisCricetinaeCytokinesDisease ProgressionDNA, ComplementaryExpressed Sequence TagsGene Expression ProfilingGene Expression RegulationGene OntologyHumansInterferon-gammaLeishmania donovaniLeishmaniasis, VisceralMesocricetusMolecular Sequence AnnotationOligonucleotide Array Sequence AnalysisPrincipal Component AnalysisRNA, MessengerSignal TransductionSpleenUp-RegulationConceptsSyrian golden hamstersVisceral leishmaniasisHamster modelGolden hamstersProgressive visceral leishmaniasisIL-4/ILIFN-γ responsesAlternative macrophage activationProgression of diseaseStudy of immunopathogenesisHamster infection modelPolarization of macrophagesImmune cell traffickingImmune response genesImmune-related genesSpleen responseImmunopathological featuresMassive splenomegalyInfected groupMacrophage activationIntracellular protozoanCell traffickingType 1Infection modelLeishmaniasis
2010
Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis
Espitia CM, Zhao W, Saldarriaga O, Osorio Y, Harrison LM, Cappello M, Travi BL, Melby PC. Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis. BMC Immunology 2010, 11: 31. PMID: 20569429, PMCID: PMC2909172, DOI: 10.1186/1471-2172-11-31.Peer-Reviewed Original ResearchConceptsReal-time RT-PCR assaysReal-time reverse transcriptase PCRRT-PCR assaysReverse transcriptase-PCRCutaneous leishmaniasisType 2 cytokine responsesNew World cutaneous leishmaniasisMRNA expressionDistinct immunological featuresCytokine mRNA expressionChemokine ligand 22Analysis of interleukinChemokine receptor 4Transcriptase-PCRReal-time RT-PCRImmune response moleculesMechanisms of diseaseIL-21IL-10Cytokine responsesFoxp3 expressionIL-12p40Immunological featuresIL-13Duplex real-time RT-PCR assay