2010
Allosteric inhibition of macrophage migration inhibitory factor revealed by ibudilast
Cho Y, Crichlow GV, Vermeire JJ, Leng L, Du X, Hodsdon ME, Bucala R, Cappello M, Gross M, Gaeta F, Johnson K, Lolis EJ. Allosteric inhibition of macrophage migration inhibitory factor revealed by ibudilast. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 11313-11318. PMID: 20534506, PMCID: PMC2895110, DOI: 10.1073/pnas.1002716107.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorInhibitory factorPeripheral blood mononuclear cellsBlood mononuclear cellsNeuropathic painOpioid withdrawalMIF receptorBronchial asthmaOcular indicationsMononuclear cellsProinflammatory proteinsAntiinflammatory drugsGlial cellsPossible therapeuticsAntiinflammatory activityChemotactic functionNonselective inhibitorAV411Major receptorTautomerase activityReceptorsAllosteric binding siteAntibody experimentsTreatment
2007
Structural and Functional Characterization of a Secreted Hookworm Macrophage Migration Inhibitory Factor (MIF) That Interacts with the Human MIF Receptor CD74*
Cho Y, Jones BF, Vermeire JJ, Leng L, DiFedele L, Harrison LM, Xiong H, Kwong YK, Chen Y, Bucala R, Lolis E, Cappello M. Structural and Functional Characterization of a Secreted Hookworm Macrophage Migration Inhibitory Factor (MIF) That Interacts with the Human MIF Receptor CD74*. Journal Of Biological Chemistry 2007, 282: 23447-23456. PMID: 17567581, PMCID: PMC3707627, DOI: 10.1074/jbc.m702950200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntigens, Differentiation, B-LymphocyteCricetinaeCytokinesHistocompatibility Antigens Class IIHumansIntestinal MucosaMacrophage Migration-Inhibitory FactorsMesocricetusMolecular Sequence DataProtein BindingRabbitsRecombinant ProteinsSequence Homology, Amino AcidConceptsMacrophage migration inhibitory factorMIF receptor CD74Migration inhibitory factorReceptor CD74Inhibitory factorHost immune responseHuman macrophage migration inhibitory factorMIF proteinHookworm Ancylostoma ceylanicumImmune responseDisease pathogenesisHuman cytokinesMacrophage migrationAncylostoma ceylanicumMajor causeWorm survivalParasite survivalISO-1Significant differencesTautomerase activityHookworm proteinsCytokinesCD74HookwormRelative bioactivity
2004
Ancylostoma ceylanicum anticoagulant peptide-1: role of the predicted reactive site amino acid in mediating inhibition of coagulation factors Xa and VIIa
Mieszczanek J, Harrison LM, Cappello M. Ancylostoma ceylanicum anticoagulant peptide-1: role of the predicted reactive site amino acid in mediating inhibition of coagulation factors Xa and VIIa. Molecular And Biochemical Parasitology 2004, 137: 151-159. PMID: 15279961, DOI: 10.1016/j.molbiopara.2004.05.011.Peer-Reviewed Original ResearchConceptsCoagulation factor XaAmino acid sequence similarityFactor XaGastrointestinal blood lossIron deficiency anemiaSite amino acid residuesAmino acid residuesSite amino acidsMechanism of actionBlood lossHookworm infectionSequence similarityDeficiency anemiaLeading causeFactor VIIa/tissue factor complexFactor complexCoagulation inhibitorsPeptide-1Ancylostoma caninum anticoagulant peptide 5Coagulation responseAcid residuesNon-active siteA. ceylanicumAnticoagulantsTissue factor complex
1998
Ancylostoma caninum Anticoagulant Peptide Blocks Metastasis In Vivo and Inhibits Factor Xa Binding to Melanoma Cells In Vitro
Donnelly K, Bromberg M, Milstone A, Madison McNiff J, Terwilliger G, Konigsberg W, Cappello M. Ancylostoma caninum Anticoagulant Peptide Blocks Metastasis In Vivo and Inhibits Factor Xa Binding to Melanoma Cells In Vitro. Thrombosis And Haemostasis 1998, 79: 1041-1047. PMID: 9609244, DOI: 10.1055/s-0037-1615117.Peer-Reviewed Original ResearchConceptsAnti-metastatic activityThrombin generationAntimetastatic effectFactor XaEffector cell protease receptor-1Melanoma cellsTissue factor pathway inhibitorDose-dependent reductionFactor pathway inhibitorHuman coagulation factor XaHuman melanoma cellsFactor V/VaPulmonary metastasesSubcutaneous injectionSCID miceCoagulation factor XaVein injectionBlock metastasisReceptor 1Inhibits factor XaPathway inhibitorTumor cellsXa concentrationsProthrombinase activityPotential mechanisms