2004
Disruption of Ixodes scapularis anticoagulation by using RNA interference
Narasimhan S, Montgomery RR, DePonte K, Tschudi C, Marcantonio N, Anderson JF, Sauer JR, Cappello M, Kantor FS, Fikrig E. Disruption of Ixodes scapularis anticoagulation by using RNA interference. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 1141-1146. PMID: 14745044, PMCID: PMC337020, DOI: 10.1073/pnas.0307669100.Peer-Reviewed Original ResearchConceptsAnti-factor Xa activityNumerous infectious diseasesIxodes scapularis ticksRNA interferenceMultiple anticoagulantsReduction of mRNAXa activityVaccine candidatesInfectious diseasesTick salivaAnticoagulantsBabesia microtiPhysiologic functionI. scapularis salivaSalivary glandsBorrelia burgdorferiTick feedingScapularis ticksAnticoagulant activityAnaplasma phagocytophilumTick engorgementSuch interventionsEngorgement weightEngorgementKey target
2001
Molecular Characterization of Ancylostoma Inhibitors of Coagulation Factor Xa HOOKWORM ANTICOAGULANT ACTIVITY IN VITRO PREDICTS PARASITE BLOODFEEDING IN VIVO *
Harrison LM, Nerlinger A, Bungiro RD, Córdova JL, Kuzmič P, Cappello M. Molecular Characterization of Ancylostoma Inhibitors of Coagulation Factor Xa HOOKWORM ANTICOAGULANT ACTIVITY IN VITRO PREDICTS PARASITE BLOODFEEDING IN VIVO *. Journal Of Biological Chemistry 2001, 277: 6223-6229. PMID: 11741914, DOI: 10.1074/jbc.m109908200.Peer-Reviewed Original ResearchConceptsCoagulation factor XaAdult A. ceylanicumHookworm speciesHuman vaccine developmentIron deficiency anemiaAnticoagulant activityFactor Xa inhibitory activityFactor Xa activityDog hookworm Ancylostoma caninumFactor XaXa inhibitory activityMechanism of actionFirst anticoagulantGastrointestinal hemorrhageDeficiency anemiaReverse transcription-PCRSpecies of hookwormXa activityPeptide-1Vaccine developmentHookworm Ancylostoma caninumA. ceylanicumAncylostoma ceylanicumHookwormAncylostoma caninumAncylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic
Harrison L, Córdova J, Cappello M. Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic. Molecular And Biochemical Parasitology 2001, 115: 101-107. PMID: 11377744, DOI: 10.1016/s0166-6851(01)00276-6.Peer-Reviewed Original ResearchConceptsPolyclonal IgGAnticoagulant activityFuture immunization strategiesGastrointestinal blood lossIron deficiency anemiaPartial thromboplastin timeFactor Xa activityHuman coagulation factor XaBlood lossDeficiency anemiaHookworm infectionThromboplastin timeXa activityAmphidial glandsAdult Ancylostoma caninum hookwormsCoagulation factor XaAdult hookwormsImmunization strategiesA. ceylanicumIgGMajor causeHookwormSerine protease inhibitorAdult wormsViable target