2001
Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic
Harrison L, Córdova J, Cappello M. Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic. Molecular And Biochemical Parasitology 2001, 115: 101-107. PMID: 11377744, DOI: 10.1016/s0166-6851(01)00276-6.Peer-Reviewed Original ResearchConceptsPolyclonal IgGAnticoagulant activityFuture immunization strategiesGastrointestinal blood lossIron deficiency anemiaPartial thromboplastin timeFactor Xa activityHuman coagulation factor XaBlood lossDeficiency anemiaHookworm infectionThromboplastin timeXa activityAmphidial glandsAdult Ancylostoma caninum hookwormsCoagulation factor XaAdult hookwormsImmunization strategiesA. ceylanicumIgGMajor causeHookwormSerine protease inhibitorAdult wormsViable target
1993
Ancylostoma Factor Xa Inhibitor: Partial Purification and Its Identification as a Major Hookworm-Derived Anticoagulant In Vitro
Cappello M, Clyne L, McPhedran P, Hotez P. Ancylostoma Factor Xa Inhibitor: Partial Purification and Its Identification as a Major Hookworm-Derived Anticoagulant In Vitro. The Journal Of Infectious Diseases 1993, 167: 1474-1477. PMID: 8501344, DOI: 10.1093/infdis/167.6.1474.Peer-Reviewed Original ResearchConceptsPotent factor Xa inhibitorsIon-exchange column chromatographyColumn chromatographyAnticoagulant activityAncylostoma hookwormsPartial thromboplastin timePeptide substratesColumn fractionsProthrombin timeChromogenic peptide substratesPartial purificationAnti-Xa activityFactor Xa inhibitorsChromatographyTime assaysPurificationSoluble protein extractsThromboplastin timeXa inhibitors