Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms
Bungiro RD, Solis CV, Harrison LM, Cappello M. Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms. Infection And Immunity 2004, 72: 2203-2213. PMID: 15039344, PMCID: PMC375217, DOI: 10.1128/iai.72.4.2203-2213.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, OralAmino Acid SequenceAncylostomaAncylostomiasisAnimalsAntibodies, HelminthAntigens, HelminthBase SequenceCloning, MolecularCricetinaeHelminth ProteinsImmunizationImmunization, PassiveInjections, SubcutaneousMesocricetusMolecular Sequence DataRecombinant ProteinsSequence Analysis, DNAVaccinesConceptsAdult worm RNANovel immunoreactive proteinsCDNA predictsImmunization of hamstersES productsEnzyme-linked immunosorbentProtein 2Immunoreactive proteinExcretory-secretory productsHookworm anaemiaOral immunizationGlobal morbidityReverse transcription-PCRInfected hamstersInduced antibodiesHigh-pressure liquid chromatographyReverse-phase high-pressure liquid chromatographyNormal serumAdult hookwormsComplete recoveryVaccinationHyperimmune rabbitAnemiaImmunizationAdult worms