2014
Molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm Ancylostoma ceylanicum
Fairfax KC, Harrison LM, Cappello M. Molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm Ancylostoma ceylanicum. Molecular And Biochemical Parasitology 2014, 198: 37-44. PMID: 25481749, PMCID: PMC4354846, DOI: 10.1016/j.molbiopara.2014.11.005.Peer-Reviewed Original ResearchConceptsAmino acid sequenceFatty acids de novoShares sequence identityExcretory/secretory proteinsPseudocoelomic spaceMolecular cloningCis-parinaric acidPartial cDNASequence identityAcid sequenceFatty acidsSimilar proteinsSecretory proteinsHookworm biologyRecombinant subunitsSoluble adult worm extractProteinAntigens/allergensSubunits
2006
Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: Evidence for a role in hookworm reproductive physiology
Brown AC, Harrison LM, Kapulkin W, Jones BF, Sinha A, Savage A, Villalon N, Cappello M. Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: Evidence for a role in hookworm reproductive physiology. Molecular And Biochemical Parasitology 2006, 151: 141-147. PMID: 17129620, PMCID: PMC1831819, DOI: 10.1016/j.molbiopara.2006.10.017.Peer-Reviewed Original ResearchConceptsC-type lectinRecombinant proteinsAmino acid mature proteinDrosophila S2 cellsPutative C-type lectinReproductive physiologySecretory signal sequenceEssential cell functionsSoluble protein extractsEgg cell membraneS2 cellsMature proteinCDNA correspondingProtein familyCDNA correspondsSignal sequenceMolecular cloningSperm proteinsRelated proteinsReverse transcription-PCRProtein extractsCell membraneProteinTranscription-PCRCell function
2003
Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum
Del Valle A, Jones BF, Harrison LM, Chadderdon RC, Cappello M. Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum. Molecular And Biochemical Parasitology 2003, 129: 167-177. PMID: 12850261, DOI: 10.1016/s0166-6851(03)00121-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntibodies, HelminthChromatography, High Pressure LiquidCloning, MolecularCollagenDNA, ComplementaryFibrinogenHelminth ProteinsImmunoglobulin GImmunohistochemistryIntegrin alpha2beta1Molecular Sequence DataPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexRecombinant ProteinsSpectrometry, Mass, Electrospray IonizationConceptsIron deficiency anemiaCell surface integrin receptorsIdentical N-terminalAmino acid sequence homologyAmino acid sequenceSingle gene productSurface integrin receptorsDeficiency anemiaMammalian hemostasisCorresponding native proteinsCDNA correspondingSignificant homologyMolecular cloningSimilar molecular massSecreted proteinsGene productsSequence homologyAcid sequenceInhibitor proteinHPI proteinVaccine-based strategiesNative proteinN-terminalAdult Ancylostoma caninumAdult stage
1996
Anticoagulant repertoire of the hookworm Ancylostoma caninum.
Stassens P, Bergum P, Gansemans Y, Jespers L, Laroche Y, Huang S, Maki S, Messens J, Lauwereys M, Cappello M, Hotez P, Lasters I, Vlasuk G. Anticoagulant repertoire of the hookworm Ancylostoma caninum. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 2149-2154. PMID: 8700900, PMCID: PMC39925, DOI: 10.1073/pnas.93.5.2149.Peer-Reviewed Original ResearchConceptsHookworm Ancylostoma caninumReactive site residuesFVIIa/TFBlood coagulation factor VIIaBlood coagulation factor XaMolecular cloningSerine protease inhibitorMammalian hostsCysteine residuesHost hemostasisSite residuesCoagulation factor VIIaHomologous regionsSequence positionsAncylostoma caninumNematode AscarisCatalytic centerBinary complexUnique mechanismCoagulation factor XaProtease inhibitorsResiduesInhibitorsTissue factorThird form