2001
Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic
Harrison L, Córdova J, Cappello M. Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic. Molecular And Biochemical Parasitology 2001, 115: 101-107. PMID: 11377744, DOI: 10.1016/s0166-6851(01)00276-6.Peer-Reviewed Original ResearchConceptsPolyclonal IgGAnticoagulant activityFuture immunization strategiesGastrointestinal blood lossIron deficiency anemiaPartial thromboplastin timeFactor Xa activityHuman coagulation factor XaBlood lossDeficiency anemiaHookworm infectionThromboplastin timeXa activityAmphidial glandsAdult Ancylostoma caninum hookwormsCoagulation factor XaAdult hookwormsImmunization strategiesA. ceylanicumIgGMajor causeHookwormSerine protease inhibitorAdult wormsViable target
1998
Ancylostoma caninum Anticoagulant Peptide Blocks Metastasis In Vivo and Inhibits Factor Xa Binding to Melanoma Cells In Vitro
Donnelly K, Bromberg M, Milstone A, Madison McNiff J, Terwilliger G, Konigsberg W, Cappello M. Ancylostoma caninum Anticoagulant Peptide Blocks Metastasis In Vivo and Inhibits Factor Xa Binding to Melanoma Cells In Vitro. Thrombosis And Haemostasis 1998, 79: 1041-1047. PMID: 9609244, DOI: 10.1055/s-0037-1615117.Peer-Reviewed Original ResearchConceptsAnti-metastatic activityThrombin generationAntimetastatic effectFactor XaEffector cell protease receptor-1Melanoma cellsTissue factor pathway inhibitorDose-dependent reductionFactor pathway inhibitorHuman coagulation factor XaHuman melanoma cellsFactor V/VaPulmonary metastasesSubcutaneous injectionSCID miceCoagulation factor XaVein injectionBlock metastasisReceptor 1Inhibits factor XaPathway inhibitorTumor cellsXa concentrationsProthrombinase activityPotential mechanisms
1995
Ancylostoma caninum anticoagulant peptide: a hookworm-derived inhibitor of human coagulation factor Xa.
Cappello M, Vlasuk G, Bergum P, Huang S, Hotez P. Ancylostoma caninum anticoagulant peptide: a hookworm-derived inhibitor of human coagulation factor Xa. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 6152-6156. PMID: 7597095, PMCID: PMC41660, DOI: 10.1073/pnas.92.13.6152.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsArthropod ProteinsBlood CoagulationChromatography, GelChromatography, High Pressure LiquidChromatography, Ion ExchangeFactor Xa InhibitorsHelminth ProteinsHirudinsHumansIntercellular Signaling Peptides and ProteinsKineticsMass SpectrometryMolecular Sequence DataPeptide FragmentsPeptidesProthrombin TimeRecombinant ProteinsSerine Proteinase InhibitorsTicksConceptsBlood lossFactor XaIntestinal blood lossGastrointestinal blood lossHuman hookworm infectionIron deficiency anemiaSoil-transmitted helminthsHuman coagulation factor XaFree factor XaAnticoagulant peptideSpecific inhibitorHookworm infectionDeficiency anemiaProthrombin timeIntestinal mucosaCoagulation factor XaMajor causeRecombinant hirudinChromogenic assayMajor anticoagulantAdult wormsAnticoagulantsProtease inhibitorsHuman coagulationInhibitors