Featured Publications
Application of multiplex amplicon deep-sequencing (MAD-seq) to screen for putative drug resistance markers in the Necator americanus isotype-1 β-tubulin gene
George S, Suwondo P, Akorli J, Otchere J, Harrison LM, Bilguvar K, Knight JR, Humphries D, Wilson MD, Caccone A, Cappello M. Application of multiplex amplicon deep-sequencing (MAD-seq) to screen for putative drug resistance markers in the Necator americanus isotype-1 β-tubulin gene. Scientific Reports 2022, 12: 11459. PMID: 35794459, PMCID: PMC9259660, DOI: 10.1038/s41598-022-15718-1.Peer-Reviewed Original ResearchConceptsSingle nucleotide polymorphismsPeriodic mass drug administrationHigh-risk groupCross-sectional studyDrug resistance markersMass drug administrationResistance-associated mutationsHookworm Necator americanusPost-treatment samplesIsotype-1 β-tubulin geneHookworm infectionPersistent infectionResistance markersDrug AdministrationNecator americanusInfection statusVeterinary nematodesInfectionMarkersNucleotide polymorphismsSensitive toolBenzimidazole drugsNucleotide alleles
2023
The effect of single dose albendazole (400 mg) treatment on the human gut microbiome of hookworm-infected Ghanaian individuals
Appiah-Twum F, Akorli J, Okyere L, Sagoe K, Osabutey D, Cappello M, Wilson M. The effect of single dose albendazole (400 mg) treatment on the human gut microbiome of hookworm-infected Ghanaian individuals. Scientific Reports 2023, 13: 11302. PMID: 37438457, PMCID: PMC10338455, DOI: 10.1038/s41598-023-38376-3.Peer-Reviewed Original ResearchConceptsHookworm infectionAlbendazole treatmentMicrobiota compositionStool samplesGut microbiomeGut microbiome dysbiosisInfection cure rateKintampo North MunicipalityPre-treatment statePotential microbial biomarkersAdjunct treatmentAnthelminthic therapySingle doseCure rateMicrobiome dysbiosisTreatment outcomesGut homeostasisPharmacological responseProbiotic supplementationGut microbiotaUninfected individualsCommensal bacteriaInfected individualsTherapy outcomeHuman gut microbiome
2022
Plasmodium falciparum coinfection is associated with improved IgE and IgG3 response against hookworm antigens
Sakyi SA, Wilson MD, Adu B, Opoku S, Brewoo A, Larbi A, Baafour EK, Tchum SK, Saahene RO, Aniagyei W, Sewor C, Courtin D, Cappello M, Gyan B, Amoani B. Plasmodium falciparum coinfection is associated with improved IgE and IgG3 response against hookworm antigens. Health Science Reports 2022, 5: e672. PMID: 35734341, PMCID: PMC9195015, DOI: 10.1002/hsr2.672.Peer-Reviewed Original ResearchL3 stage larvaeAlbendazole treatmentHookworm infectionImmune responseNonendemic controlsPlasmodium falciparum coinfectionsIron deficiency anemiaHookworm treatmentVaccine effectivenessIgG3 responsesIgG responsesAntibody profileSingle doseDeficiency anemiaIgG3 antibodiesBlood samplesIgEProtein malnutritionControl groupVaccine developmentHigh levelsSerum samplesIgG3Stage larvaeInfection
2021
Effect of hookworm infection and anthelmintic treatment on naturally acquired antibody responses against the GMZ2 malaria vaccine candidate and constituent antigens
Amoani B, Gyan B, Sakyi SA, Abu EK, Nuvor SV, Barnes P, Sarkodie-Addo T, Ahenkorah B, Sewor C, Dwomoh D, Theisen M, Cappello M, Wilson MD, Adu B. Effect of hookworm infection and anthelmintic treatment on naturally acquired antibody responses against the GMZ2 malaria vaccine candidate and constituent antigens. BMC Infectious Diseases 2021, 21: 332. PMID: 33832450, PMCID: PMC8028774, DOI: 10.1186/s12879-021-06027-5.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAlbendazoleAnthelminticsAntibodies, ProtozoanAntigens, ProtozoanCase-Control StudiesChildChild, PreschoolCross-Sectional StudiesFemaleHookworm InfectionsHumansImmunoglobulin GLongitudinal StudiesMalaria VaccinesMalaria, FalciparumMaleMiddle AgedParasitemiaProtozoan ProteinsYoung AdultConceptsMerozoite surface protein 3Antibody responseHookworm infectionMalaria vaccine candidateConstituent antigensP. falciparumGLURP-R0IgG3 levelsVaccine candidatesLongitudinal cross-sectional studyHigher IgG3 levelsUninfected endemic controlsP. falciparum infectionPlasmodium falciparum merozoite surface protein 3Levels of IgG1Kato-Katz methodCross-sectional studyHigher antibody responseMalaria vaccine efficacyKintampo North MunicipalityAnthelmintic treatmentHookworm treatmentMalaria parasitaemiaAlbendazole treatmentEndemic controls
2019
Genetic Markers of Benzimidazole Resistance among Human Hookworms (Necator americanus) in Kintampo North Municipality, Ghana.
Orr AR, Quagraine JE, Suwondo P, George S, Harrison LM, Dornas FP, Evans B, Caccone A, Humphries D, Wilson MD, Cappello M. Genetic Markers of Benzimidazole Resistance among Human Hookworms (Necator americanus) in Kintampo North Municipality, Ghana. American Journal Of Tropical Medicine And Hygiene 2019, 100: 351-356. PMID: 30734697, PMCID: PMC6367626, DOI: 10.4269/ajtmh.18-0727.Peer-Reviewed Original ResearchConceptsMass drug administrationSoil-transmitted helminthsKintampo North MunicipalitySchool-aged childrenSingle nucleotide polymorphismsPolymerase chain reactionEffectiveness of MDAWild-type SNPsInfected study subjectsPeriodic mass drug administrationResistance-associated single nucleotide polymorphismsCross-sectional studyResistance-associated mutationsWorld Health OrganizationHookworm infectionDrug exposureHookworm eggsUnreported associationStudy subjectsDrug AdministrationPosttreatment samplesChemotherapeutic approachesGrowth delayHuman hookwormHealth Organization
2018
Albendazole Treatment Improves Work Capacity in Women Smallholder Farmers Infected with Hookworm: A Double-Blind Randomized Control Trial.
Salmon M, Salmon C, Masoda M, Salumu JM, Bozzi C, Nieburg P, Harrison LM, Humphries D, Abaca Uvon N, Wendel SK, Trout C, Cappello M. Albendazole Treatment Improves Work Capacity in Women Smallholder Farmers Infected with Hookworm: A Double-Blind Randomized Control Trial. American Journal Of Tropical Medicine And Hygiene 2018, 98: 1419-1426. PMID: 29611504, PMCID: PMC5953358, DOI: 10.4269/ajtmh.17-0403.Peer-Reviewed Original ResearchConceptsWork capacityRandomized Effectiveness TrialSoil-transmitted helminthsHealth of womenStep testPlacebo groupAlbendazole treatmentPrimary outcomeHookworm infectionStep test resultsEffectiveness trialNonsignificant decreaseHeart rateControl trialTreatment groupsIntestinal parasitesPulse rateHookwormTrialsRisk benefitDrug distributionWomenTreatmentAlbendazoleMinutes
2016
Effectiveness of Albendazole for Hookworm Varies Widely by Community and Correlates with Nutritional Factors: A Cross-Sectional Study of School-Age Children in Ghana.
Humphries D, Nguyen S, Kumar S, Quagraine JE, Otchere J, Harrison LM, Wilson M, Cappello M. Effectiveness of Albendazole for Hookworm Varies Widely by Community and Correlates with Nutritional Factors: A Cross-Sectional Study of School-Age Children in Ghana. American Journal Of Tropical Medicine And Hygiene 2016, 96: 347-354. PMID: 27895280, PMCID: PMC5303035, DOI: 10.4269/ajtmh.16-0682.Peer-Reviewed Original ResearchConceptsEgg reduction rateCross-sectional studyMass drug administrationSchool-aged childrenEffectiveness of albendazoleCure rateDrug AdministrationMid-upper arm circumferenceSoil-transmitted helminth infectionsHost factorsNutritional factorsSingle-dose albendazoleOverall cure rateBlood hemoglobin levelsKintampo North MunicipalitySignificant community-level variationIndividual host factorsWorld Health OrganizationCommon STHHemoglobin levelsArm circumferenceHookworm infectionHelminth infectionsDeworming programsHookworm prevalence
2013
Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection
Wiśniewski M, Jaros S, Bąska P, Cappello M, Wędrychowicz H. Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection. Acta Parasitologica 2013, 58: 376-383. PMID: 23990436, DOI: 10.2478/s11686-013-0151-9.Peer-Reviewed Original ResearchConceptsChallenge infectionAnthelminthic drug resistanceEffective human vaccineWorm burden reductionsGroups of hamstersIron deficiency anemiaCDNA vaccineTriple immunizationInfected childrenDeficiency anemiaDNA vaccineHookworm infectionDNA vaccinationHuman vaccinesAnimal modelsNumerous antigensVaccine developmentGrowth retardationIntestinal nematodesDrug resistanceVaccineBurden reductionPartial protectionInfectionHemoglobin digestionHookworm Infection among School Age Children in Kintampo North Municipality, Ghana: Nutritional Risk Factors and Response to Albendazole Treatment
Humphries D, Simms BT, Davey D, Otchere J, Quagraine J, Terryah S, Newton S, Berg E, Harrison LM, Boakye D, Wilson M, Cappello M. Hookworm Infection among School Age Children in Kintampo North Municipality, Ghana: Nutritional Risk Factors and Response to Albendazole Treatment. American Journal Of Tropical Medicine And Hygiene 2013, 89: 540-548. PMID: 23836564, PMCID: PMC3771297, DOI: 10.4269/ajtmh.12-0605.Peer-Reviewed Original ResearchConceptsKintampo North MunicipalityHookworm infectionCure rateSchool-aged childrenModest cure ratesSingle-dose albendazoleNutritional risk factorsLevels of antibodiesYears of ageAge childrenMalaria parasitemiaAlbendazole treatmentRisk factorsChildren 6InfectionInfection statusHealth careSignificant predictorsAnimal foodsHookwormAlbendazoleFurther investigationAgeChildrenLow consumption
2012
Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum
Davey D, Manickam N, Simms BT, Harrison LM, Vermeire JJ, Cappello M. Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum. Experimental Parasitology 2012, 133: 243-249. PMID: 23232252, PMCID: PMC3580025, DOI: 10.1016/j.exppara.2012.11.010.Peer-Reviewed Original ResearchConceptsExperimental infectionThird-stage hookworm larvaeParasite-specific IgAHumoral immune responseStudy of pathogenesisResource-limited countriesHuman hookworm diseaseHookworm infectionLifelong susceptibilityPrimary infectionImmune responseProlonged susceptibilitySubsequent challengeAntibody productionVaccine developmentHookworm larvaeHookworm diseaseIntestinal nematodesInfectionPathological effectsRepeated exposureAncylostoma ceylanicumContinued susceptibilityMajor causeMediate resistanceOxadiazole 2-oxides are toxic to the human hookworm, Ancylostoma ceylanicum, however glutathione reductase is not the primary target
Treger R, Cook A, Rai G, Maloney D, Simeonov A, Jadhav A, Thomas C, Williams D, Cappello M, Vermeire J. Oxadiazole 2-oxides are toxic to the human hookworm, Ancylostoma ceylanicum, however glutathione reductase is not the primary target. International Journal For Parasitology Drugs And Drug Resistance 2012, 2: 171-177. PMID: 22844653, PMCID: PMC3404738, DOI: 10.1016/j.ijpddr.2012.05.001.Peer-Reviewed Original ResearchAncylostoma ceylanicumVivo anthelminthic efficacyIntestinal worm burdenEffective drug therapyGlutathione reductasePrimary targetOral treatmentSevere anemiaDrug therapyHookworm infectionHookworm Ancylostoma ceylanicumAntischistosomal compoundsHamster modelNovel chemotherapyTrematode parasite Schistosoma mansoniWorm burdenGrowth delayHookworm diseaseParasite Schistosoma mansoniEx vivoParasitic diseasesParasite deathA. ceylanicumSchistosoma mansoniWeight gainNutritional risk factors for hookworm infection among school age children in the Kintampo North Municipality, Ghana
Humphries D, Simms B, Davey D, Otchere J, Quagraine J, Berg E, Newton S, Harrison L, Boakye D, Wilson M, Cappello M. Nutritional risk factors for hookworm infection among school age children in the Kintampo North Municipality, Ghana. The FASEB Journal 2012, 26: 1028.6-1028.6. DOI: 10.1096/fasebj.26.1_supplement.1028.6.Peer-Reviewed Original ResearchHookworm infectionKintampo North MunicipalityRisk factorsInfection statusSchool-aged childrenNutritional risk factorsDietary risk factorsMalaria infection statusSignificant risk factorsDietary diversityDegree of stuntingLow-intensity infectionsChildren ages 6Recent dewormingClinical sequelaeResults PrevalenceHigh prevalenceFood groupsIntensity infectionsNutritional statusCross-sectional sampleHookwormInfectionAnemiaHAZ scores
2011
Epidemiology of Hookworm Infection in Kintampo North Municipality, Ghana: Patterns of Malaria Coinfection, Anemia, and Albendazole Treatment Failure
Humphries D, Mosites E, Otchere J, Twum WA, Woo L, Jones-Sanpei H, Harrison LM, Bungiro RD, Benham-Pyle B, Bimi L, Edoh D, Bosompem K, Wilson M, Cappello M. Epidemiology of Hookworm Infection in Kintampo North Municipality, Ghana: Patterns of Malaria Coinfection, Anemia, and Albendazole Treatment Failure. American Journal Of Tropical Medicine And Hygiene 2011, 84: 792-800. PMID: 21540391, PMCID: PMC3083749, DOI: 10.4269/ajtmh.2011.11-0003.Peer-Reviewed Original ResearchConceptsPoor nutritional statusHookworm infectionRisk factorsNutritional statusOverall egg reduction rateSerum immunoglobulin G (IgG) reactivitiesCross-sectional pilot studyEgg reduction rateImmunoglobulin G reactivityKintampo North MunicipalityAlbendazole therapyKintampo NorthMalaria coinfectionTreatment failureOverall prevalenceHigh prevalenceRepeat examinationEgg excretionSecretory antigensInfectionPilot studyCoinfectionSignificant reductionTherapyHigh rateTwenty-First Century Progress Toward the Global Control of Human Hookworm Infection
Bungiro R, Cappello M. Twenty-First Century Progress Toward the Global Control of Human Hookworm Infection. Current Infectious Disease Reports 2011, 13: 210. PMID: 21462001, DOI: 10.1007/s11908-011-0182-z.Peer-Reviewed Original ResearchHuman hookworm infectionHost immune responseCandidate vaccine antigensHigher infection rateExcretory/secretory proteinsImmunoepidemiological studiesTreatment failureHookworm infectionMass chemotherapyImmune responseAnimal modelsParasitic infectionsVaccine antigensMolecular pathogenesisInfection rateHookworm diseaseReduced efficacyProtease inhibitorsHookwormInfectionSaharan AfricaRecent studiesContinued useSecretory proteinsAsthma
2010
CD4+ T cells mediate mucosal and systemic immune responses to experimental hookworm infection
DONDJI B, SUN T, BUNGIRO RD, VERMEIRE JJ, HARRISON LM, BIFULCO C, CAPPELLO M. CD4+ T cells mediate mucosal and systemic immune responses to experimental hookworm infection. Parasite Immunology 2010, 32: 406-413. PMID: 20500671, PMCID: PMC3241967, DOI: 10.1111/j.1365-3024.2010.01204.x.Peer-Reviewed Original ResearchConceptsT cellsImmune responseHookworm infectionHost cellular immune responseExperimental hookworm infectionT-cell depletionMesenteric lymph nodesSystemic immune responsesCellular immune responsesHumoral immune responseIntestinal nematode infectionsIntestinal worm burdenResource-limited countriesHookworm antigensLymph nodesSevere anemiaIntraperitoneal injectionLymphocyte proliferationHookworm Ancylostoma ceylanicumIsotype controlDisease pathogenesisWorm burdenSyrian hamstersAncylostoma ceylanicumEffector role
2008
Mucosal antibody responses in experimental hookworm infection
BUNGIRO RD, SUN T, HARRISON LM, SHOEMAKER CB, CAPPELLO M. Mucosal antibody responses in experimental hookworm infection. Parasite Immunology 2008, 30: 293-303. PMID: 18312503, DOI: 10.1111/j.1365-3024.2008.01023.x.Peer-Reviewed Original ResearchConceptsMucosal IgA responsesSystemic immune responsesIgA responsesImmune responseHookworm infectionInfected hamstersRobust systemic immune responseAncylostoma ceylanicum infectionExperimental hookworm infectionFecal IgA responsesParasite-specific IgAMucosal immune responsesMucosal antibody responsesIntestinal worm burdenExcretory-secretory productsMucosal antibodiesProtective immunityChallenge infectionSerum IgGAntibody responsePrimary infectionSecretory IgAHamster modelIntestinal mucosaWorm burdenRole for Nitric Oxide in Hookworm-Associated Immune Suppression
Dondji B, Bungiro RD, Harrison LM, Vermeire JJ, Bifulco C, McMahon-Pratt D, Cappello M. Role for Nitric Oxide in Hookworm-Associated Immune Suppression. Infection And Immunity 2008, 76: 2560-2567. PMID: 18347036, PMCID: PMC2423093, DOI: 10.1128/iai.00094-08.Peer-Reviewed Original ResearchConceptsAntigen-presenting cellsHookworm infectionNitric oxideInfected animalsMesenteric lymph node cellsHost cellular immune responseCellular immune responsesLymph node cellsProliferative capacityT cell preparationsSurface immunoglobulin GParasite-induced immunosuppressionResource-poor countriesHookworm antigensMLN cellsLymphocyte subpopulationsPositive lymphocytesCellular immunityImmune suppressionLymphocyte proliferationNode cellsFluorescence-activated cell sortingInfected hamstersImmune responseAnimal studies
2006
Dietary Iron Content Mediates Hookworm Pathogenesis In Vivo
Held MR, Bungiro RD, Harrison LM, Hamza I, Cappello M. Dietary Iron Content Mediates Hookworm Pathogenesis In Vivo. Infection And Immunity 2006, 74: 289-295. PMID: 16368983, PMCID: PMC1346670, DOI: 10.1128/iai.74.1.289-295.2006.Peer-Reviewed Original ResearchConceptsDay 20 postinfectionGrowth delayIron-restricted dietHigh-iron dietIntestinal worm burdenDietary iron restrictionIron deficiency anemiaBlood hemoglobin levelsDietary iron contentGreater weight lossSignificant growth delayHemoglobin levelsDeficiency anemiaHookworm infectionDiet groupHamster modelSevere diseaseStandard dietUninfected controlsHemoglobin concentrationUninfected animalsWorm burdenDay 10Infected animalsHookworm disease
2005
Detection of excretory/secretory coproantigens in experimental hookworm infection.
Bungiro RD, Cappello M. Detection of excretory/secretory coproantigens in experimental hookworm infection. American Journal Of Tropical Medicine And Hygiene 2005, 73: 915-20. PMID: 16282303, DOI: 10.4269/ajtmh.2005.73.915.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayMolecular host-parasite interactionsFuture diagnostic methodsExcretory/secretoryFecal extractsImmunosorbent assayA. ceylanicum infectionExperimental hookworm infectionIntestinal worm burdenRabbit polyclonal IgG antibodyPolyclonal IgG antibodiesES proteinsDiagnostic methodsHookworm antigensDetectionHookworm infectionIgG antibodiesStool samplesWorm burdenDay 17Hamster serumCoproantigenFaecal eggMug/Degrees C.
2004
Hookworm infection: new developments and prospects for control
Bungiro R, Cappello M. Hookworm infection: new developments and prospects for control. Current Opinion In Infectious Diseases 2004, 17: 421-426. PMID: 15353961, DOI: 10.1097/00001432-200410000-00006.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHookworm infectionHookworm diseaseEffective global controlPathological immune responsesMajor health burdenParasite virulence factorsNew chemotherapeutic agentsDevelopment of vaccinesHookworm anaemiaImmunoepidemiological studiesMass chemotherapyHealth burdenImmune responseMolecular pathogenesisGrowth delayChemotherapeutic agentsInfectionVaccineSocioeconomic statusDiseaseVirulence factorsGlobal healthBenzimidazole anthelminthicsPathogenesisAnthelminthic resistance