Featured Publications
Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response
Bungiro RD, Harrison LM, Dondji B, Cappello M. Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response. PLOS Neglected Tropical Diseases 2022, 16: e0010098. PMID: 34986139, PMCID: PMC8765627, DOI: 10.1371/journal.pntd.0010098.Peer-Reviewed Original ResearchConceptsCellular immune responsesGolden Syrian hamstersImmune responsePercutaneous infectionOral infectionSyrian hamstersAncylostoma ceylanicumPrimary immune responseRoute of infectionModerate growth delayFaecal egg outputPercutaneous groupImmunological manifestationsAntibody titersHookworm Ancylostoma ceylanicumInfected hamstersWorm recoveryLarval antigensBlood hemoglobinDisease pathogenesisHost responseGrowth delayHuman infectionsInfectionA. ceylanicum
2013
In vitro Screening of Compounds against Laboratory and Field Isolates of Human Hookworm Reveals Quantitative Differences in Anthelmintic Susceptibility
Treger RS, Otchere J, Keil MF, Quagraine JE, Rai G, Mott BT, Humphries DL, Wilson M, Cappello M, Vermeire JJ. In vitro Screening of Compounds against Laboratory and Field Isolates of Human Hookworm Reveals Quantitative Differences in Anthelmintic Susceptibility. American Journal Of Tropical Medicine And Hygiene 2013, 90: 71-74. PMID: 24297811, PMCID: PMC3886431, DOI: 10.4269/ajtmh.12-0547.Peer-Reviewed Original ResearchConceptsField isolatesA. ceylanicumHookwormScreening of compoundsAncylostoma ceylanicumHuman hookwormAnthelmintic compoundsLaboratory strainsHookworm speciesPercent inhibitionSchool childrenTwofold increaseSelect compoundsAnthelmintic activityNovel anthelmintic compoundsAnthelmintic susceptibilityBrong Ahafo RegionIsolatesAhafo RegionCloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins
Siwińska A, Bąska P, Daniłowicz-Luebert E, Januszkiewicz K, Długosz E, Wędrychowicz H, Cappello M, Wiśniewski M. Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins. Acta Parasitologica 2013, 58: 112-118. PMID: 23377920, DOI: 10.2478/s11686-013-0116-z.Peer-Reviewed Original Research
2012
Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum
Davey D, Manickam N, Simms BT, Harrison LM, Vermeire JJ, Cappello M. Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum. Experimental Parasitology 2012, 133: 243-249. PMID: 23232252, PMCID: PMC3580025, DOI: 10.1016/j.exppara.2012.11.010.Peer-Reviewed Original ResearchConceptsExperimental infectionThird-stage hookworm larvaeParasite-specific IgAHumoral immune responseStudy of pathogenesisResource-limited countriesHuman hookworm diseaseHookworm infectionLifelong susceptibilityPrimary infectionImmune responseProlonged susceptibilitySubsequent challengeAntibody productionVaccine developmentHookworm larvaeHookworm diseaseIntestinal nematodesInfectionPathological effectsRepeated exposureAncylostoma ceylanicumContinued susceptibilityMajor causeMediate resistanceOxadiazole 2-oxides are toxic to the human hookworm, Ancylostoma ceylanicum, however glutathione reductase is not the primary target
Treger R, Cook A, Rai G, Maloney D, Simeonov A, Jadhav A, Thomas C, Williams D, Cappello M, Vermeire J. Oxadiazole 2-oxides are toxic to the human hookworm, Ancylostoma ceylanicum, however glutathione reductase is not the primary target. International Journal For Parasitology Drugs And Drug Resistance 2012, 2: 171-177. PMID: 22844653, PMCID: PMC3404738, DOI: 10.1016/j.ijpddr.2012.05.001.Peer-Reviewed Original ResearchAncylostoma ceylanicumVivo anthelminthic efficacyIntestinal worm burdenEffective drug therapyGlutathione reductasePrimary targetOral treatmentSevere anemiaDrug therapyHookworm infectionHookworm Ancylostoma ceylanicumAntischistosomal compoundsHamster modelNovel chemotherapyTrematode parasite Schistosoma mansoniWorm burdenGrowth delayHookworm diseaseParasite Schistosoma mansoniEx vivoParasitic diseasesParasite deathA. ceylanicumSchistosoma mansoniWeight gainA furoxan – amodiaquine hybrid as a potential therapeutic for three parasitic diseases
Mott BT, Cheng KC, Guha R, Kommer VP, Williams DL, Vermeire JJ, Cappello M, Maloney DJ, Rai G, Jadhav A, Simeonov A, Inglese J, Posner GH, Thomas CJ. A furoxan – amodiaquine hybrid as a potential therapeutic for three parasitic diseases. RSC Medicinal Chemistry 2012, 3: 1505-1511. PMID: 23205265, PMCID: PMC3509744, DOI: 10.1039/c2md20238g.Peer-Reviewed Original ResearchParasitic diseasesEx vivo assaysAntimalarial drug amodiaquineNovel therapiesEffective treatmentParasite Plasmodium falciparumSchistosoma mansoniAncylostoma ceylanicumParasitic targetsPotential therapeuticsMultiple mechanismsVivo assaysDiseaseFrequent emergenceAmodiaquineTherapyHuman populationMansoniFalciparum
2011
Ancylostoma ceylanicum Excretory–Secretory Protein 2 Adopts a Netrin-Like Fold and Defines a Novel Family of Nematode Proteins
Kucera K, Harrison LM, Cappello M, Modis Y. Ancylostoma ceylanicum Excretory–Secretory Protein 2 Adopts a Netrin-Like Fold and Defines a Novel Family of Nematode Proteins. Journal Of Molecular Biology 2011, 408: 9-17. PMID: 21352830, PMCID: PMC3070796, DOI: 10.1016/j.jmb.2011.02.033.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAncylostomatoideaAncylostomiasisAnimalsComplement System ProteinsHelminth ProteinsHumansMatrix MetalloproteinasesMolecular Sequence DataNerve Growth FactorsNetrin-1Protein ConformationProtein FoldingSequence Homology, Amino AcidTissue Inhibitor of MetalloproteinasesTumor Suppressor ProteinsConceptsMatrix metalloproteasesHost immune responseComplement factor C3Nematode proteinsProtein 2Complement-mediated cell lysisHookworm anaemiaMucosal vaccinesImmune responseUseful model organismFactor C3Decoy receptorDisease pathogenesisTissue inhibitorIntestinal attachmentNovel familyAncylostoma ceylanicumImmunoreactive moleculesAdult wormsGlobal healthModel organismsHookwormSequence homologyÅ resolutionHuman parasites
2010
CD4+ T cells mediate mucosal and systemic immune responses to experimental hookworm infection
DONDJI B, SUN T, BUNGIRO RD, VERMEIRE JJ, HARRISON LM, BIFULCO C, CAPPELLO M. CD4+ T cells mediate mucosal and systemic immune responses to experimental hookworm infection. Parasite Immunology 2010, 32: 406-413. PMID: 20500671, PMCID: PMC3241967, DOI: 10.1111/j.1365-3024.2010.01204.x.Peer-Reviewed Original ResearchConceptsT cellsImmune responseHookworm infectionHost cellular immune responseExperimental hookworm infectionT-cell depletionMesenteric lymph nodesSystemic immune responsesCellular immune responsesHumoral immune responseIntestinal nematode infectionsIntestinal worm burdenResource-limited countriesHookworm antigensLymph nodesSevere anemiaIntraperitoneal injectionLymphocyte proliferationHookworm Ancylostoma ceylanicumIsotype controlDisease pathogenesisWorm burdenSyrian hamstersAncylostoma ceylanicumEffector role
2009
Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum
Fairfax KC, Vermeire JJ, Harrison LM, Bungiro RD, Grant W, Husain SZ, Cappello M. Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum. International Journal For Parasitology 2009, 39: 1561-1571. PMID: 19591834, PMCID: PMC2760681, DOI: 10.1016/j.ijpara.2009.06.005.Peer-Reviewed Original ResearchConceptsMucosal adjuvant cholera toxinAdjuvant cholera toxinCause of anemiaLive adult wormsIntestinal worm burdenFatty acidsResource limited countriesFatty acid uptakeHookworm Ancylostoma ceylanicumReal-time PCRFatty acids de novoWorm burdenVaccine developmentLimited countriesIntestinal nematodesEx vivoA. ceylanicumCholera toxinHookworm biologyIndividual fatty acidsAncylostoma ceylanicumAcid uptakeAdult wormsProtein 1Potential role
2007
Short report: an agar plate method for culturing hookworm larvae: analysis of growth kinetics and infectivity compared with standard coproculture techniques.
Reiss D, Harrison LM, Bungiro R, Cappello M. Short report: an agar plate method for culturing hookworm larvae: analysis of growth kinetics and infectivity compared with standard coproculture techniques. American Journal Of Tropical Medicine And Hygiene 2007, 77: 1087-90. PMID: 18165527, DOI: 10.4269/ajtmh.2007.77.1087.Peer-Reviewed Original ResearchStructural and Functional Characterization of a Secreted Hookworm Macrophage Migration Inhibitory Factor (MIF) That Interacts with the Human MIF Receptor CD74*
Cho Y, Jones BF, Vermeire JJ, Leng L, DiFedele L, Harrison LM, Xiong H, Kwong YK, Chen Y, Bucala R, Lolis E, Cappello M. Structural and Functional Characterization of a Secreted Hookworm Macrophage Migration Inhibitory Factor (MIF) That Interacts with the Human MIF Receptor CD74*. Journal Of Biological Chemistry 2007, 282: 23447-23456. PMID: 17567581, PMCID: PMC3707627, DOI: 10.1074/jbc.m702950200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntigens, Differentiation, B-LymphocyteCricetinaeCytokinesHistocompatibility Antigens Class IIHumansIntestinal MucosaMacrophage Migration-Inhibitory FactorsMesocricetusMolecular Sequence DataProtein BindingRabbitsRecombinant ProteinsSequence Homology, Amino AcidConceptsMacrophage migration inhibitory factorMIF receptor CD74Migration inhibitory factorReceptor CD74Inhibitory factorHost immune responseHuman macrophage migration inhibitory factorMIF proteinHookworm Ancylostoma ceylanicumImmune responseDisease pathogenesisHuman cytokinesMacrophage migrationAncylostoma ceylanicumMajor causeWorm survivalParasite survivalISO-1Significant differencesTautomerase activityHookworm proteinsCytokinesCD74HookwormRelative bioactivity
2006
A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostoma ceylanicum
Cappello M, Bungiro RD, Harrison LM, Bischof LJ, Griffitts JS, Barrows BD, Aroian RV. A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostoma ceylanicum. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 15154-15159. PMID: 17005719, PMCID: PMC1622792, DOI: 10.1073/pnas.0607002103.Peer-Reviewed Original ResearchConceptsCry proteinsCrystal proteinsSoil bacterium Bacillus thuringiensisBacterium Bacillus thuringiensisBacillus thuringiensis crystal proteinsBt Cry proteinsVivo anthelminthic activityFree-living nematodesA. ceylanicumLarval development assaysAdult A. ceylanicumGastrointestinal nematodesBacillus thuringiensisParasitic nematodesNematode parasitesVeterinary useCry5BAncylostoma ceylanicumNematodesDevelopment assaysEgg excretionAdult female wormsHuman diseasesWorm burdenPermissive host
2001
Molecular Characterization of Ancylostoma Inhibitors of Coagulation Factor Xa HOOKWORM ANTICOAGULANT ACTIVITY IN VITRO PREDICTS PARASITE BLOODFEEDING IN VIVO *
Harrison LM, Nerlinger A, Bungiro RD, Córdova JL, Kuzmič P, Cappello M. Molecular Characterization of Ancylostoma Inhibitors of Coagulation Factor Xa HOOKWORM ANTICOAGULANT ACTIVITY IN VITRO PREDICTS PARASITE BLOODFEEDING IN VIVO *. Journal Of Biological Chemistry 2001, 277: 6223-6229. PMID: 11741914, DOI: 10.1074/jbc.m109908200.Peer-Reviewed Original ResearchConceptsCoagulation factor XaAdult A. ceylanicumHookworm speciesHuman vaccine developmentIron deficiency anemiaAnticoagulant activityFactor Xa inhibitory activityFactor Xa activityDog hookworm Ancylostoma caninumFactor XaXa inhibitory activityMechanism of actionFirst anticoagulantGastrointestinal hemorrhageDeficiency anemiaReverse transcription-PCRSpecies of hookwormXa activityPeptide-1Vaccine developmentHookworm Ancylostoma caninumA. ceylanicumAncylostoma ceylanicumHookwormAncylostoma caninumMitigation of Hookworm Disease by Immunization with Soluble Extracts of Ancylostoma ceylanicum
Bungiro R, Greene J, Kruglov E, Cappello M. Mitigation of Hookworm Disease by Immunization with Soluble Extracts of Ancylostoma ceylanicum. The Journal Of Infectious Diseases 2001, 183: 1380-1387. PMID: 11294670, DOI: 10.1086/319867.Peer-Reviewed Original ResearchConceptsHookworm infectionMajor clinical sequelaeHuman vaccine developmentCause of anemiaSyrian golden hamstersAdult worm burdenGlobal control effortsClinical sequelaeActive vaccinationPrimary infectionWorm burdenAdult hookwormsGrowth delayVaccine developmentHookworm diseaseGolden hamstersPooled seraAncylostoma ceylanicumPartial protectionAnemiaWeight lossAbsence of reductionInfectionAdult parasitesHamsters