2016
Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection
Wiśniewski M, Jaros S, Bąska P, Cappello M, Długosz E, Wędrychowicz H. Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection. Experimental Parasitology 2016, 163: 1-7. PMID: 26795262, DOI: 10.1016/j.exppara.2016.01.006.Peer-Reviewed Original ResearchConceptsA. ceylanicum infectionAncylostoma ceylanicum infectionHookworm speciesWorm burden reductionsSyrian golden hamstersProtective immunityDNA vaccineGut mucosaGastrointestinal tractIntestinal nematodesGolden hamstersBurden reductionAdult wormsHemoglobin digestionEgg countsImmunizationVaccineInfectionHamstersMetalloproteasesMucosaHookwormBloodTractImmunity
2011
Ancylostoma ceylanicum Excretory–Secretory Protein 2 Adopts a Netrin-Like Fold and Defines a Novel Family of Nematode Proteins
Kucera K, Harrison LM, Cappello M, Modis Y. Ancylostoma ceylanicum Excretory–Secretory Protein 2 Adopts a Netrin-Like Fold and Defines a Novel Family of Nematode Proteins. Journal Of Molecular Biology 2011, 408: 9-17. PMID: 21352830, PMCID: PMC3070796, DOI: 10.1016/j.jmb.2011.02.033.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAncylostomatoideaAncylostomiasisAnimalsComplement System ProteinsHelminth ProteinsHumansMatrix MetalloproteinasesMolecular Sequence DataNerve Growth FactorsNetrin-1Protein ConformationProtein FoldingSequence Homology, Amino AcidTissue Inhibitor of MetalloproteinasesTumor Suppressor ProteinsConceptsMatrix metalloproteasesHost immune responseComplement factor C3Nematode proteinsProtein 2Complement-mediated cell lysisHookworm anaemiaMucosal vaccinesImmune responseUseful model organismFactor C3Decoy receptorDisease pathogenesisTissue inhibitorIntestinal attachmentNovel familyAncylostoma ceylanicumImmunoreactive moleculesAdult wormsGlobal healthModel organismsHookwormSequence homologyÅ resolutionHuman parasites
2009
Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum
Fairfax KC, Vermeire JJ, Harrison LM, Bungiro RD, Grant W, Husain SZ, Cappello M. Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum. International Journal For Parasitology 2009, 39: 1561-1571. PMID: 19591834, PMCID: PMC2760681, DOI: 10.1016/j.ijpara.2009.06.005.Peer-Reviewed Original ResearchConceptsMucosal adjuvant cholera toxinAdjuvant cholera toxinCause of anemiaLive adult wormsIntestinal worm burdenFatty acidsResource limited countriesFatty acid uptakeHookworm Ancylostoma ceylanicumReal-time PCRFatty acids de novoWorm burdenVaccine developmentLimited countriesIntestinal nematodesEx vivoA. ceylanicumCholera toxinHookworm biologyIndividual fatty acidsAncylostoma ceylanicumAcid uptakeAdult wormsProtein 1Potential role
2007
Short report: an agar plate method for culturing hookworm larvae: analysis of growth kinetics and infectivity compared with standard coproculture techniques.
Reiss D, Harrison LM, Bungiro R, Cappello M. Short report: an agar plate method for culturing hookworm larvae: analysis of growth kinetics and infectivity compared with standard coproculture techniques. American Journal Of Tropical Medicine And Hygiene 2007, 77: 1087-90. PMID: 18165527, DOI: 10.4269/ajtmh.2007.77.1087.Peer-Reviewed Original Research
2004
Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms
Bungiro RD, Solis CV, Harrison LM, Cappello M. Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms. Infection And Immunity 2004, 72: 2203-2213. PMID: 15039344, PMCID: PMC375217, DOI: 10.1128/iai.72.4.2203-2213.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, OralAmino Acid SequenceAncylostomaAncylostomiasisAnimalsAntibodies, HelminthAntigens, HelminthBase SequenceCloning, MolecularCricetinaeHelminth ProteinsImmunizationImmunization, PassiveInjections, SubcutaneousMesocricetusMolecular Sequence DataRecombinant ProteinsSequence Analysis, DNAVaccinesConceptsAdult worm RNANovel immunoreactive proteinsCDNA predictsImmunization of hamstersES productsEnzyme-linked immunosorbentProtein 2Immunoreactive proteinExcretory-secretory productsHookworm anaemiaOral immunizationGlobal morbidityReverse transcription-PCRInfected hamstersInduced antibodiesHigh-pressure liquid chromatographyReverse-phase high-pressure liquid chromatographyNormal serumAdult hookwormsComplete recoveryVaccinationHyperimmune rabbitAnemiaImmunizationAdult worms
2003
Oral Transfer of Adult Ancylostoma ceylanicum Hookworms into Permissive and Nonpermissive Host Species
Bungiro RD, Anderson BR, Cappello M. Oral Transfer of Adult Ancylostoma ceylanicum Hookworms into Permissive and Nonpermissive Host Species. Infection And Immunity 2003, 71: 1880-1886. PMID: 12654804, PMCID: PMC152085, DOI: 10.1128/iai.71.4.1880-1886.2003.Peer-Reviewed Original ResearchConceptsBALB/c miceHigh-dose groupHumoral immune responseCD-1 miceLong-term survivalRecovery of parasitesExcretory-secretory productsHemoglobin levelsPrechallenge levelsC57BL/6 miceHookworm infectionC miceWeanling hamstersOral infectionImmune responseMurine modelHookworm larvaeSyrian hamstersMouse strainsTypical infectionsInfectionPrior exposureMiceAdult wormsOral transfer
2001
Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic
Harrison L, Córdova J, Cappello M. Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic. Molecular And Biochemical Parasitology 2001, 115: 101-107. PMID: 11377744, DOI: 10.1016/s0166-6851(01)00276-6.Peer-Reviewed Original ResearchConceptsPolyclonal IgGAnticoagulant activityFuture immunization strategiesGastrointestinal blood lossIron deficiency anemiaPartial thromboplastin timeFactor Xa activityHuman coagulation factor XaBlood lossDeficiency anemiaHookworm infectionThromboplastin timeXa activityAmphidial glandsAdult Ancylostoma caninum hookwormsCoagulation factor XaAdult hookwormsImmunization strategiesA. ceylanicumIgGMajor causeHookwormSerine protease inhibitorAdult wormsViable target
1999
The Hookworm Platelet Inhibitor: Functional Blockade of Integrins GPIIb/IIIa (αIIbβ3) and GPIa/IIa (α2β1) Inhibits Platelet Aggregation and Adhesion In Vitro
Chadderdon R, Cappello M. The Hookworm Platelet Inhibitor: Functional Blockade of Integrins GPIIb/IIIa (αIIbβ3) and GPIa/IIa (α2β1) Inhibits Platelet Aggregation and Adhesion In Vitro. The Journal Of Infectious Diseases 1999, 179: 1235-1241. PMID: 10191228, DOI: 10.1086/314724.Peer-Reviewed Original ResearchConceptsIntegrin GPIIb/IIIaPlatelet inhibitorsSoluble protein extractsGPIIb/IIIaPlatelet aggregationMolecular massProtein extractsIron deficiency anemiaGPIa/IIaInhibitory monoclonal antibodiesVariety of agonistsAnti-platelet activityAdult Ancylostoma caninum hookwormsInhibits platelet aggregationPlatelet receptorsDeficiency anemiaFunctional blockadeSpecific blockadeBiologic roleSize exclusion chromatographyIntestinal nematodesSecretory productsMonoclonal antibodiesInhibitorsAdult worms
1995
Ancylostoma caninum anticoagulant peptide: a hookworm-derived inhibitor of human coagulation factor Xa.
Cappello M, Vlasuk G, Bergum P, Huang S, Hotez P. Ancylostoma caninum anticoagulant peptide: a hookworm-derived inhibitor of human coagulation factor Xa. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 6152-6156. PMID: 7597095, PMCID: PMC41660, DOI: 10.1073/pnas.92.13.6152.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsArthropod ProteinsBlood CoagulationChromatography, GelChromatography, High Pressure LiquidChromatography, Ion ExchangeFactor Xa InhibitorsHelminth ProteinsHirudinsHumansIntercellular Signaling Peptides and ProteinsKineticsMass SpectrometryMolecular Sequence DataPeptide FragmentsPeptidesProthrombin TimeRecombinant ProteinsSerine Proteinase InhibitorsTicksConceptsBlood lossFactor XaIntestinal blood lossGastrointestinal blood lossHuman hookworm infectionIron deficiency anemiaSoil-transmitted helminthsHuman coagulation factor XaFree factor XaAnticoagulant peptideSpecific inhibitorHookworm infectionDeficiency anemiaProthrombin timeIntestinal mucosaCoagulation factor XaMajor causeRecombinant hirudinChromogenic assayMajor anticoagulantAdult wormsAnticoagulantsProtease inhibitorsHuman coagulationInhibitors
1994
Hyaluronidases Of The Gastrointestinal Invasive Nematodes Ancylostoma Caninum And Anisakis Simplex: Possible Functions In The Pathogenesis Of Human Zoonoses
Hotez P, Cappello M, Hawdon J, Beckers C, Sakanari J. Hyaluronidases Of The Gastrointestinal Invasive Nematodes Ancylostoma Caninum And Anisakis Simplex: Possible Functions In The Pathogenesis Of Human Zoonoses. The Journal Of Infectious Diseases 1994, 170: 918-926. PMID: 7930737, DOI: 10.1093/infdis/170.4.918.Peer-Reviewed Original Research