2014
Molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm Ancylostoma ceylanicum
Fairfax KC, Harrison LM, Cappello M. Molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm Ancylostoma ceylanicum. Molecular And Biochemical Parasitology 2014, 198: 37-44. PMID: 25481749, PMCID: PMC4354846, DOI: 10.1016/j.molbiopara.2014.11.005.Peer-Reviewed Original ResearchConceptsAmino acid sequenceFatty acids de novoShares sequence identityExcretory/secretory proteinsPseudocoelomic spaceMolecular cloningCis-parinaric acidPartial cDNASequence identityAcid sequenceFatty acidsSimilar proteinsSecretory proteinsHookworm biologyRecombinant subunitsSoluble adult worm extractProteinAntigens/allergensSubunits
2003
Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum
Del Valle A, Jones BF, Harrison LM, Chadderdon RC, Cappello M. Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum. Molecular And Biochemical Parasitology 2003, 129: 167-177. PMID: 12850261, DOI: 10.1016/s0166-6851(03)00121-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntibodies, HelminthChromatography, High Pressure LiquidCloning, MolecularCollagenDNA, ComplementaryFibrinogenHelminth ProteinsImmunoglobulin GImmunohistochemistryIntegrin alpha2beta1Molecular Sequence DataPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexRecombinant ProteinsSpectrometry, Mass, Electrospray IonizationConceptsIron deficiency anemiaCell surface integrin receptorsIdentical N-terminalAmino acid sequence homologyAmino acid sequenceSingle gene productSurface integrin receptorsDeficiency anemiaMammalian hemostasisCorresponding native proteinsCDNA correspondingSignificant homologyMolecular cloningSimilar molecular massSecreted proteinsGene productsSequence homologyAcid sequenceInhibitor proteinHPI proteinVaccine-based strategiesNative proteinN-terminalAdult Ancylostoma caninumAdult stage
2002
A novel family of anticoagulants from the saliva of Ixodes scapularis
Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E. A novel family of anticoagulants from the saliva of Ixodes scapularis. Insect Molecular Biology 2002, 11: 641-650. PMID: 12421422, DOI: 10.1046/j.1365-2583.2002.00375.x.Peer-Reviewed Original ResearchConceptsN-terminal amino acid sequenceSalivary gland cDNA libraryAmino acid sequenceN-terminal sequenceCDNA libraryAcid sequenceAnticoagulant proteinsIxodes scapularisMolecular approachesTerminal sequenceIntrinsic pathwayEscherichia coliSpecific inhibitorNovel familyProteinSalp14Different functionsTick salivaSequenceBiological activityDose-dependent mannerProtease inhibitorsReversed-phase HPLCParaloguesScapularis