2013
MMP13 is a critical target gene during the progression of osteoarthritis
Wang M, Sampson ER, Jin H, Li J, Ke QH, Im HJ, Chen D. MMP13 is a critical target gene during the progression of osteoarthritis. Arthritis Research & Therapy 2013, 15: r5. PMID: 23298463, PMCID: PMC3672752, DOI: 10.1186/ar4133.Peer-Reviewed Original ResearchConceptsMeniscal-ligamentous injuryOA progressionControl miceMMP13 activityType II collagenTUNEL stainingCre-negative control miceChondrocyte apoptosisEffective disease-modifying treatmentTerminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stainingTransferase dUTP nick end labeling stainingDUTP nick end labeling stainingRole of MMP13Nick end labeling stainingArticular cartilage lossCartilage gradingDisease-modifying treatmentsProgression of osteoarthritisWild-type miceDegenerative joint diseaseDevelopment of OAResults of immunohistochemistryEnd labeling stainingCre-negative miceArticular cartilage degeneration
2012
Lead induces an osteoarthritis‐like phenotype in articular chondrocytes through disruption of TGF‐β signaling
Holz JD, Beier E, Sheu T, Ubayawardena R, Wang M, Sampson ER, Rosier RN, Zuscik M, Puzas JE. Lead induces an osteoarthritis‐like phenotype in articular chondrocytes through disruption of TGF‐β signaling. Journal Of Orthopaedic Research® 2012, 30: 1760-1766. PMID: 22517267, PMCID: PMC3839422, DOI: 10.1002/jor.22117.Peer-Reviewed Original ResearchConceptsLead treatmentOsteoarthritis-like phenotypeNormal chondrocyte phenotypeDose-dependent mannerArticular chondrocytesTGF-β signalingActive caspase-3MMP13 activityLead exposureHigher level leadType II collagenVivo exposureCollagen levelsNovel targetType X collagenCaspase-3Articular surfaceEnvironmental toxinsLead toxicityII collagenReporter activityTreatmentArticular cartilageDosePhenotypic shift