2015
Periosteal PTHrP Regulates Cortical Bone Remodeling During Fracture Healing
Wang M, Nasiri AR, Broadus AE, Tommasini SM. Periosteal PTHrP Regulates Cortical Bone Remodeling During Fracture Healing. Bone 2015, 81: 104-111. PMID: 26164475, PMCID: PMC4641003, DOI: 10.1016/j.bone.2015.07.008.Peer-Reviewed Original ResearchConceptsFracture healingPTHrP expressionCKO miceCortical bone surfaceFracture repairTibial fracture surgeryRole of PTHrPHormone-related proteinTibial fracture modelCartilaginous callus formationConditional knockout miceBone surfaceCortical bone remodelingType I receptorCD1 controlsFracture surgeryCD1 miceKnockout miceInitial genetic evidencePTHrPOsteoblastic activityBone remodelingMiceBone mineralizationI receptor
2013
Deletion of the Transforming Growth Factor β Receptor Type II Gene in Articular Chondrocytes Leads to a Progressive Osteoarthritis‐like Phenotype in Mice
Shen J, Li J, Wang B, Jin H, Wang M, Zhang Y, Yang Y, Im H, O'Keefe R, Chen D. Deletion of the Transforming Growth Factor β Receptor Type II Gene in Articular Chondrocytes Leads to a Progressive Osteoarthritis‐like Phenotype in Mice. Arthritis & Rheumatism 2013, 65: 3107-3119. PMID: 23982761, PMCID: PMC3928444, DOI: 10.1002/art.38122.Peer-Reviewed Original ResearchMeSH KeywordsADAM ProteinsADAMTS5 ProteinAnimalsCartilage, ArticularChondrocytesCore Binding Factor Alpha 2 SubunitDisease ProgressionMatrix Metalloproteinase 13MiceMice, KnockoutOsteoarthritisPhenotypeProtein Serine-Threonine KinasesReceptor, Transforming Growth Factor-beta Type IIReceptors, Transforming Growth Factor betaSignal TransductionUp-RegulationConceptsCritical downstream target genesDownstream target genesTarget genesGrowth factor β signalingRegulation of Runx2Mouse genetic approachesLoss of TGFβType II geneGene expression analysisInhibition of TGFβDevelopment of osteoarthritisRat chondrosarcoma cellsGenetic approachesExpression analysisConditional knockout miceTGFβ signalingOsteoarthritis-like phenotypeGenesΒ signalingADAMTS5 geneReceptor type IIChondrosarcoma cellsTGFβCartilage homeostasisOA-like phenotype
2011
BMP2, but not BMP4, is crucial for chondrocyte proliferation and maturation during endochondral bone development
Shu B, Zhang M, Xie R, Wang M, Jin H, Hou W, Tang D, Harris SE, Mishina Y, O'Keefe RJ, Hilton MJ, Wang Y, Chen D. BMP2, but not BMP4, is crucial for chondrocyte proliferation and maturation during endochondral bone development. Journal Of Cell Science 2011, 124: 3428-3440. PMID: 21984813, PMCID: PMC3196857, DOI: 10.1242/jcs.083659.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBone DevelopmentBone Morphogenetic Protein 2Bone Morphogenetic Protein 4Cell DifferentiationCell Growth ProcessesCells, CulturedChondrocytesCore Binding Factor Alpha 1 SubunitCyclin-Dependent Kinase 4Gene Expression RegulationGrowth PlateMiceMice, KnockoutOsteochondrodysplasiasProtein Processing, Post-TranslationalSignal TransductionConceptsEndochondral bone developmentBMP4 geneCartilage developmentDeletion of Bmp2Post-transcriptional levelRunx2 protein levelsBone developmentChondrocyte proliferationChondrodysplasia phenotypeProteasomal degradationBMP2 geneGenetic controlKey regulatorConditional knockout miceMolecular mechanismsKnockout miceBMP2Chondrocyte differentiationGenesGrowth plate chondrocytesRunx2 expressionCartilage phenotypeSpecific functionsProfound defectsNovel insightsSmad1 plays an essential role in bone development and postnatal bone formation
Wang M, Jin H, Tang D, Huang S, Zuscik MJ, Chen D. Smad1 plays an essential role in bone development and postnatal bone formation. Osteoarthritis And Cartilage 2011, 19: 751-762. PMID: 21420501, PMCID: PMC3113680, DOI: 10.1016/j.joca.2011.03.004.Peer-Reviewed Original ResearchConceptsPostnatal bone formationBone developmentCKO miceAlkaline phosphatase activityCalvarial bone developmentRole of Smad1Essential roleGene expression analysisBone formationOsteoblast-specific deletionCol2a1-Cre transgenic miceTransgenic miceBMP signalingSmad1 geneRelative gene expressionExpression analysisGene expressionConditional knockout miceOsteopenic phenotypeSmad1Formation assaysPhosphatase activityPrimary cellsOsteoblast proliferationKnockout miceTGF‐β signaling plays an essential role in the growth and maintenance of intervertebral disc tissue
Jin H, Shen J, Wang B, Wang M, Shu B, Chen D. TGF‐β signaling plays an essential role in the growth and maintenance of intervertebral disc tissue. FEBS Letters 2011, 585: 1209-1215. PMID: 21420963, PMCID: PMC3090135, DOI: 10.1016/j.febslet.2011.03.034.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Density Conservation AgentsCells, CulturedChondrocytesCollagen Type IIFemaleGrowth PlateHistocytochemistryIntervertebral DiscMaleMatrix Metalloproteinase 13MiceMice, KnockoutMice, TransgenicProtein Serine-Threonine KinasesReceptor, Transforming Growth Factor-beta Type IIReceptors, Transforming Growth Factor betaReverse Transcriptase Polymerase Chain ReactionSignal TransductionTamoxifenTime FactorsConceptsKnockout miceTgfbr2 conditional knockout miceMMP13 geneDisc tissueConditional knockout miceGrowth plate chondrocytesIntervertebral disc tissueAnnulus fibrosus cellsTGFBR2 geneTransgenic miceGrowth plate cartilagePostnatal stagesMiceNormal growth plate cartilagePlate cartilageDisc phenotypesDisc cellsNull backgroundPresent studyCartilage growthTissueCartilageCritical roleCellsEmbryonic stages