2016
Improved Determination of Subnuclear Position Enabled by Three-Dimensional Membrane Reconstruction
Zhao Y, Schreiner SM, Koo PK, Colombi P, King MC, Mochrie SG. Improved Determination of Subnuclear Position Enabled by Three-Dimensional Membrane Reconstruction. Biophysical Journal 2016, 111: 19-24. PMID: 27410730, PMCID: PMC4945324, DOI: 10.1016/j.bpj.2016.05.036.Peer-Reviewed Original ResearchConceptsChromatin biologyLac operator arraysMembrane marker proteinsNuclear positionBroad practical utilityNuclear volumeNuclear peripheryOrganelle shapeChromatin markersTranscriptional regulationSubnuclear positionLacI-GFPDNA repairIndividual lociNuclear compartmentPopulation of cellsNuclear envelopeLocus positionParticular locusMarker proteinsImage analysis pipelineLociCell populationsAnalysis pipelineBiological accuracy
2015
Nuclear–cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions
Stewart RM, Zubek AE, Rosowski KA, Schreiner SM, Horsley V, King MC. Nuclear–cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions. Journal Of Cell Biology 2015, 209: 403-418. PMID: 25963820, PMCID: PMC4427780, DOI: 10.1083/jcb.201502024.Peer-Reviewed Original ResearchConceptsIntercellular adhesionNuclear positionEpidermal tissue integrityLinker of nucleoskeletonCross talkPrimary mouse keratinocytesCytoskeleton (LINC) complexCytoplasmic cytoskeletonAdhesion functionMicrotubule networkDefective adhesionCytoskeletonSUN2Mouse keratinocytesTissue integrityFollicle structureEpidermal keratinocytesAdhesionNucleoskeletonCellsKeratinocytesAdhesion formationNucleusIntegrityComplexes