2023
Acetyl-methyllysine marks chromatin at active transcription start sites
Lu-Culligan W, Connor L, Xie Y, Ekundayo B, Rose B, Machyna M, Pintado-Urbanc A, Zimmer J, Vock I, Bhanu N, King M, Garcia B, Bleichert F, Simon M. Acetyl-methyllysine marks chromatin at active transcription start sites. Nature 2023, 622: 173-179. PMID: 37731000, PMCID: PMC10845139, DOI: 10.1038/s41586-023-06565-9.Peer-Reviewed Original ResearchConceptsPost-translational modificationsLysine residuesActive transcription start sitesTranscription start siteRange of speciesChromatin biologyChromatin proteinsLysine methylationActive chromatinProteins BRD2Transcriptional initiationLysine acetylationHistone H4Start siteMammalian tissuesHuman diseasesSame residuesMethylationAcetylationChromatinResiduesProteinBiological signalsHistonesBRD2
2016
Improved Determination of Subnuclear Position Enabled by Three-Dimensional Membrane Reconstruction
Zhao Y, Schreiner SM, Koo PK, Colombi P, King MC, Mochrie SG. Improved Determination of Subnuclear Position Enabled by Three-Dimensional Membrane Reconstruction. Biophysical Journal 2016, 111: 19-24. PMID: 27410730, PMCID: PMC4945324, DOI: 10.1016/j.bpj.2016.05.036.Peer-Reviewed Original ResearchConceptsChromatin biologyLac operator arraysMembrane marker proteinsNuclear positionBroad practical utilityNuclear volumeNuclear peripheryOrganelle shapeChromatin markersTranscriptional regulationSubnuclear positionLacI-GFPDNA repairIndividual lociNuclear compartmentPopulation of cellsNuclear envelopeLocus positionParticular locusMarker proteinsImage analysis pipelineLociCell populationsAnalysis pipelineBiological accuracy