Single-Cell and Population Transcriptomics Reveal Pan-epithelial Remodeling in Type 2-High Asthma
Jackson ND, Everman JL, Chioccioli M, Feriani L, Goldfarbmuren KC, Sajuthi SP, Rios CL, Powell R, Armstrong M, Gomez J, Michel C, Eng C, Oh SS, Rodriguez-Santana J, Cicuta P, Reisdorph N, Burchard EG, Seibold MA. Single-Cell and Population Transcriptomics Reveal Pan-epithelial Remodeling in Type 2-High Asthma. Cell Reports 2020, 32: 107872. PMID: 32640237, PMCID: PMC8046336, DOI: 10.1016/j.celrep.2020.107872.Peer-Reviewed Original ResearchConceptsIL-13Single-cell RNA sequencingHuman airway epithelial cell culturesCell typesIL-13 effectsAirway epithelial cell culturesNasal airway epitheliumCandidate therapeutic targetTranscriptional statesAsthmatic childrenAsthma endotypesRNA sequencingMucus obstructionAirway epitheliumSecretory programMucin productionNormal epithelial functionTherapeutic targetEpithelial cell culturesCellular remodelingER stressCell deathEpithelial functionMetaplastic stateSecretory cells