2021
Obtaining Xenopus tropicalis Eggs.
Lane M, Mis EK, Khokha MK. Obtaining Xenopus tropicalis Eggs. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106344. PMID: 34031209, DOI: 10.1101/pdb.prot106344.Peer-Reviewed Original ResearchConceptsDevelopmental biologyGene manipulation toolsPowerful model systemCell biological studiesCell-free systemTetraploid genomeDiploid genomeThousands of eggsEgg extractsGenetic studiesXenopusGenomePremier systemModel systemEggsBiological studiesBiologyEmbryosFrogsManipulation toolsTiming of stepsSpeciesHormoneFemalesCellsObtaining Xenopus tropicalis Embryos by In Vitro Fertilization.
Lane M, Khokha MK. Obtaining Xenopus tropicalis Embryos by In Vitro Fertilization. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106351. PMID: 34031212, DOI: 10.1101/pdb.prot106351.Peer-Reviewed Original ResearchObtaining Xenopus tropicalis Embryos by Natural Mating.
Lane M, Khokha MK. Obtaining Xenopus tropicalis Embryos by Natural Mating. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106609. PMID: 34031213, DOI: 10.1101/pdb.prot106609.Peer-Reviewed Original Research
2013
Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis
Lane MA, Kimber M, Khokha MK. Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis. PLOS ONE 2013, 8: e76807. PMID: 24116167, PMCID: PMC3792888, DOI: 10.1371/journal.pone.0076807.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedBase SequenceBinding SitesBreedingCrosses, GeneticDNA Transposable ElementsEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMaleMicroscopy, FluorescenceModels, GeneticMutagenesis, InsertionalReverse Transcriptase Polymerase Chain ReactionTransposasesXenopusConceptsTol2 transposonEnhancer-trapping strategySimple breeding strategiesTransgenic reporter linesX. tropicalisTransgenic linesGene trapBreeding strategiesReporter linesTrap strategyBiological processesTransposonDiverse arrayTransgenesisPowerful modelXenopusGenomeGermlineLinesExpressingRemobilizationTropicalisNew opportunities
2005
Identification of mutants in inbred Xenopus tropicalis
Grammer TC, Khokha MK, Lane MA, Lam K, Harland RM. Identification of mutants in inbred Xenopus tropicalis. Cells And Development 2005, 122: 263-272. PMID: 15763207, DOI: 10.1016/j.mod.2004.11.003.Peer-Reviewed Original ResearchConceptsXenopus tropicalisRecessive embryonic lethalsGenetic model organismEarly vertebrate developmentIdentification of mutantsFuture mutagenesisVertebrate developmentMutant analysisModel organismsX. tropicalisEmbryonic lethalGenetic analysisGenetic backgroundFrogsMutationsTropicalisAmphibiansMutantsMutagenesisLethalGeneticsOrganismsStrainsCrossHybrids