2021
Raising and Maintaining Xenopus tropicalis from Tadpole to Adult
Lane M, Slocum M, Khokha MK. Raising and Maintaining Xenopus tropicalis from Tadpole to Adult. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106369. PMID: 34031210, DOI: 10.1101/pdb.prot106369.Peer-Reviewed Original Research
2018
CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis
Deniz E, Mis EK, Lane M, Khokha MK. CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis. Methods In Molecular Biology 2018, 1865: 163-174. PMID: 30151766, DOI: 10.1007/978-1-4939-8784-9_12.Peer-Reviewed Original ResearchConceptsCardiac developmentCRISPR/Candidate genesHigh-density SNP arrayCRISPR/Cas9 systemGenome editing technologyCongenital heart disease genesNew genomic technologiesHeart disease genesCopy number variationsRapid functional assayXenopus tropicalisCas9 systemGenetic basisDevelopmental systemsEditing technologyGenomic technologiesSequence variationDisease genesDifferent genesGenetic analysisSNP arrayDevelopmental mechanismsMolecular mechanismsWhole-exome sequencing
2017
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ. CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Nature Communications 2017, 8: 2024. PMID: 29222508, PMCID: PMC5722943, DOI: 10.1038/s41467-017-01836-2.Peer-Reviewed Original ResearchConceptsHomology-directed repairCpf1 activityGenome editingDifferent eukaryotic systemsGenome engineering toolsEfficient homology-directed repairPost-translational modulationEctothermic organismsEctothermic speciesEukaryotic systemsDNA endonucleaseCRISPR-Cpf1Efficient mutagenesisGenomic DNADNA integrationMolecular understandingTemporal controlZebrafishAsCpf1Cpf1LbCpf1EditingNovel classGenomeMutagenesis