2021
Microinjection of Xenopus tropicalis Embryos.
Lane M, Mis EK, Khokha MK. Microinjection of Xenopus tropicalis Embryos. Cold Spring Harbor Protocols 2021, 2022: pdb.prot107644. PMID: 34244348, DOI: 10.1101/pdb.prot107644.Peer-Reviewed Original ResearchConceptsEmbryo sizeLarger embryo sizeTargeted gene manipulationEmbryo development timeSmall embryo sizeXenopus tropicalis embryosDevelopmental biologyEarly embryosMicroinjection protocolGene manipulationGenetic studiesFirst divisionXenopusEmbryosImportant modelDevelopment timeMicroinjectionMorpholinoCRISPRBiologySpeciesDNAMRNAOocytesFertilizationObtaining Xenopus tropicalis Eggs.
Lane M, Mis EK, Khokha MK. Obtaining Xenopus tropicalis Eggs. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106344. PMID: 34031209, DOI: 10.1101/pdb.prot106344.Peer-Reviewed Original ResearchConceptsDevelopmental biologyGene manipulation toolsPowerful model systemCell biological studiesCell-free systemTetraploid genomeDiploid genomeThousands of eggsEgg extractsGenetic studiesXenopusGenomePremier systemModel systemEggsBiological studiesBiologyEmbryosFrogsManipulation toolsTiming of stepsSpeciesHormoneFemalesCellsRaising and Maintaining Xenopus tropicalis from Tadpole to Adult
Lane M, Slocum M, Khokha MK. Raising and Maintaining Xenopus tropicalis from Tadpole to Adult. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106369. PMID: 34031210, DOI: 10.1101/pdb.prot106369.Peer-Reviewed Original Research
2013
Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis
Lane MA, Kimber M, Khokha MK. Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis. PLOS ONE 2013, 8: e76807. PMID: 24116167, PMCID: PMC3792888, DOI: 10.1371/journal.pone.0076807.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedBase SequenceBinding SitesBreedingCrosses, GeneticDNA Transposable ElementsEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMaleMicroscopy, FluorescenceModels, GeneticMutagenesis, InsertionalReverse Transcriptase Polymerase Chain ReactionTransposasesXenopusConceptsTol2 transposonEnhancer-trapping strategySimple breeding strategiesTransgenic reporter linesX. tropicalisTransgenic linesGene trapBreeding strategiesReporter linesTrap strategyBiological processesTransposonDiverse arrayTransgenesisPowerful modelXenopusGenomeGermlineLinesExpressingRemobilizationTropicalisNew opportunities