2021
Obtaining Xenopus tropicalis Eggs.
Lane M, Mis EK, Khokha MK. Obtaining Xenopus tropicalis Eggs. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106344. PMID: 34031209, DOI: 10.1101/pdb.prot106344.Peer-Reviewed Original ResearchConceptsDevelopmental biologyGene manipulation toolsPowerful model systemCell biological studiesCell-free systemTetraploid genomeDiploid genomeThousands of eggsEgg extractsGenetic studiesXenopusGenomePremier systemModel systemEggsBiological studiesBiologyEmbryosFrogsManipulation toolsTiming of stepsSpeciesHormoneFemalesCells
2017
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ. CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Nature Communications 2017, 8: 2024. PMID: 29222508, PMCID: PMC5722943, DOI: 10.1038/s41467-017-01836-2.Peer-Reviewed Original ResearchConceptsHomology-directed repairCpf1 activityGenome editingDifferent eukaryotic systemsGenome engineering toolsEfficient homology-directed repairPost-translational modulationEctothermic organismsEctothermic speciesEukaryotic systemsDNA endonucleaseCRISPR-Cpf1Efficient mutagenesisGenomic DNADNA integrationMolecular understandingTemporal controlZebrafishAsCpf1Cpf1LbCpf1EditingNovel classGenomeMutagenesis
2013
Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis
Lane MA, Kimber M, Khokha MK. Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis. PLOS ONE 2013, 8: e76807. PMID: 24116167, PMCID: PMC3792888, DOI: 10.1371/journal.pone.0076807.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedBase SequenceBinding SitesBreedingCrosses, GeneticDNA Transposable ElementsEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMaleMicroscopy, FluorescenceModels, GeneticMutagenesis, InsertionalReverse Transcriptase Polymerase Chain ReactionTransposasesXenopusConceptsTol2 transposonEnhancer-trapping strategySimple breeding strategiesTransgenic reporter linesX. tropicalisTransgenic linesGene trapBreeding strategiesReporter linesTrap strategyBiological processesTransposonDiverse arrayTransgenesisPowerful modelXenopusGenomeGermlineLinesExpressingRemobilizationTropicalisNew opportunities