2023
CMPK2 restricts Zika virus replication by inhibiting viral translation
Pawlak J, Hsu J, Xia H, Han P, Suh H, Grove T, Morrison J, Shi P, Cresswell P, Laurent-Rolle M. CMPK2 restricts Zika virus replication by inhibiting viral translation. PLOS Pathogens 2023, 19: e1011286. PMID: 37075076, PMCID: PMC10150978, DOI: 10.1371/journal.ppat.1011286.Peer-Reviewed Original ResearchConceptsCytidine/uridine monophosphate kinase 2I interferon-stimulated genesZika virus replicationYellow fever virusAntiviral activityAntiviral effectVirus replicationKunjin virusType I interferon-stimulated genesFirst lineOverall antiviral responseHost's first lineEffective therapeutic interventionsViral translationBroad antiviral activityInterferon-stimulated genesGlobal health threatAntiviral treatmentFlaviviral infectionsPathogenic flavivirusesAntiviral functionDrug AdministrationTherapeutic interventionsAntiviral responseDengue virus
2021
Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein
Hsu JC, Laurent-Rolle M, Pawlak JB, Wilen CB, Cresswell P. Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2101161118. PMID: 34045361, PMCID: PMC8214666, DOI: 10.1073/pnas.2101161118.Peer-Reviewed Original ResearchConceptsSARS-CoV-2Interferon-stimulated genesImmune responseSevere acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Host protein synthesisRespiratory syndrome coronavirus 2Syndrome coronavirus 2Innate immune responseUnprecedented global health crisisCoronavirus 2N7-methyltransferase activityOngoing COVID-19 pandemicHuman coronavirusesTranslational shutdownVirus replicationNsp14 proteinGlobal health crisisProtein synthesisInhibition activityCausative agentCOVID-19COVID-19 pandemicSARS-CoV-2 nsp14Dependent induction
2013
Dengue Virus Co-opts UBR4 to Degrade STAT2 and Antagonize Type I Interferon Signaling
Morrison J, Laurent-Rolle M, Maestre AM, Rajsbaum R, Pisanelli G, Simon V, Mulder LC, Fernandez-Sesma A, García-Sastre A. Dengue Virus Co-opts UBR4 to Degrade STAT2 and Antagonize Type I Interferon Signaling. PLOS Pathogens 2013, 9: e1003265. PMID: 23555265, PMCID: PMC3610674, DOI: 10.1371/journal.ppat.1003265.Peer-Reviewed Original ResearchConceptsDengue hemorrhagic feverType I interferonDengue feverSTAT2 degradationImmune evasionI interferonDENV NS5Anti-DENV therapeuticsDengue virus infectionDENV NS5 proteinVirus infectionHemorrhagic feverDENV replicationViral replicationAntiviral therapeuticsEfficient viral replicationIFNPotential severityFeverDENVInterferonNS5Competent cellsHost proteinsNS5 protein
2010
The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling
Laurent-Rolle M, Boer EF, Lubick KJ, Wolfinbarger JB, Carmody AB, Rockx B, Liu W, Ashour J, Shupert WL, Holbrook MR, Barrett AD, Mason PW, Bloom ME, García-Sastre A, Khromykh AA, Best SM. The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling. Journal Of Virology 2010, 84: 3503-3515. PMID: 20106931, PMCID: PMC2838099, DOI: 10.1128/jvi.01161-09.Peer-Reviewed Original ResearchConceptsPY-STAT1IFN antagonismJAK-STAT signal transductionIFN antagonistAlpha/beta interferonSignal transductionWild-type virusAnalogous residuesIFN-dependent gene expressionGene expressionJAK-STATKunjin virusSingle residueHost innate responseNonstructural protein NS5NS5 proteinSTAT1 phosphorylationSTAT2 degradationPoor suppressorNY99 strainPresence of IFNMutationsIFN-alpha/NS5Beta interferon
2009
NS5 of Dengue Virus Mediates STAT2 Binding and Degradation
Ashour J, Laurent-Rolle M, Shi PY, García-Sastre A. NS5 of Dengue Virus Mediates STAT2 Binding and Degradation. Journal Of Virology 2009, 83: 5408-5418. PMID: 19279106, PMCID: PMC2681973, DOI: 10.1128/jvi.02188-08.Peer-Reviewed Original ResearchConceptsDengue virusInnate immune responseInnate antiviral responseImmune responseInfection resultsIFN responseAntiviral responseDENV proteinsLevel of expressionAntiviral therapeuticsDENV genomeDecreased levelsInterferonPotential targetProtein levelsViral pathogensHost proteasesReduced levelsProteasome activityMultiple mechanismsVirusFacilitate infectionNovel mechanismViral polypeptidesNS5
2003
Inhibition of interferon signaling by dengue virus
Muñoz-Jordán J, Sánchez-Burgos GG, Laurent-Rolle M, García-Sastre A. Inhibition of interferon signaling by dengue virus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 14333-14338. PMID: 14612562, PMCID: PMC283592, DOI: 10.1073/pnas.2335168100.Peer-Reviewed Original ResearchConceptsPositive-strand RNA genomeExpression of NS4BPositive-strand RNA virusesNuclear signal transducerVirus-encoded proteinsStrand RNA virusesStrand RNA genomeHuman A549 cellsDengue virusRNA genomeDengue virus-infected cellsSignal transducerTranscription 1Gene expressionUnknown functionVirus-infected cellsInhibition of interferonRNA virusesDengue virus infectionNS4BMosquito-borne flavivirusProteinA549 cellsIFN responseViral products