2023
Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1
Abini-Agbomson S, Gretarsson K, Shih R, Hsieh L, Lou T, De Ioannes P, Vasilyev N, Lee R, Wang M, Simon M, Armache J, Nudler E, Narlikar G, Liu S, Lu C, Armache K. Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1. Molecular Cell 2023, 83: 2872-2883.e7. PMID: 37595555, DOI: 10.1016/j.molcel.2023.07.020.Peer-Reviewed Original ResearchConceptsNon-catalytic activitiesNon-catalytic mechanismHistone H4 lysine 20Histone variant H2A.ZH4 lysine 20Large macromolecular complexesCatalytic activityHeterochromatin formationHeterochromatin functionVariant H2A.ZLysine 20Nucleosome substratesGenomic stabilityDNA replicationNucleosomal DNAHistone methyltransferaseChromatin condensationSUV420H1Histone octamerMacromolecular complexesCryoelectron microscopyCellular analysisEssential roleDistinct phenotypesCrucial role
2007
The Site-Specific Installation of Methyl-Lysine Analogs into Recombinant Histones
Simon MD, Chu F, Racki LR, de la Cruz CC, Burlingame AL, Panning B, Narlikar GJ, Shokat KM. The Site-Specific Installation of Methyl-Lysine Analogs into Recombinant Histones. Cell 2007, 128: 1003-1012. PMID: 17350582, PMCID: PMC2932701, DOI: 10.1016/j.cell.2006.12.041.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCysteineHistonesLysineMethylationModels, MolecularNucleosomesRecombinant ProteinsXenopus ProteinsConceptsLysine methylationChromatin structureRecombinant histonesContext of chromatinHistone lysine methylationHistone lysine residuesSpecific lysine methylationNucleosome remodelingEffector proteinsPosttranslational modificationsDegree of methylationRecombinant proteinsHistonesLysine residuesMethyl lysineMethylationBiochemical mechanismsSite-specific installationProteinRapid generationNatural counterpartsChromatinPowerful toolAdaptorResidues