2024
A surgical protocol for establishing spinal cord ischemia with extended lifespan and low complication rates in rats
Yasuda N, Sasaki M, Kocsis J, Kawaharada N, Honmou O. A surgical protocol for establishing spinal cord ischemia with extended lifespan and low complication rates in rats. World Neurosurgery 2024, 188: e349-e356. PMID: 38789035, DOI: 10.1016/j.wneu.2024.05.114.Peer-Reviewed Original ResearchIschemic spinal cord injurySpinal cord ischemiaCord ischemiaComplication rateRat modelTherapeutic strategiesEvaluate new therapeutic strategiesFunctional recoveryMale Sprague-Dawley ratsLow complication rateSprague-Dawley ratsSpecialized surgical equipmentExperimental animal modelsImprove functional recoveryPromote functional recoverySpinal cord injuryCross-clampingSevere neurological disordersAzygos veinSurgical protocolDescending aortaBulldog clampsLumbar levelsSpinal cordIschemic lesions
2017
Intravenous Infusion of Bone Marrow–Derived Mesenchymal Stem Cells Reduces Erectile Dysfunction Following Cavernous Nerve Injury in Rats
Matsuda Y, Sasaki M, Kataoka-Sasaki Y, Takayanagi A, Kobayashi K, Oka S, Nakazaki M, Masumori N, Kocsis JD, Honmou O. Intravenous Infusion of Bone Marrow–Derived Mesenchymal Stem Cells Reduces Erectile Dysfunction Following Cavernous Nerve Injury in Rats. Sexual Medicine 2017, 6: 49-57. PMID: 29275062, PMCID: PMC5815969, DOI: 10.1016/j.esxm.2017.10.005.Peer-Reviewed Original ResearchMajor pelvic ganglionBone marrow-derived MSCsMarrow-derived mesenchymal stem cellsPostoperative erectile dysfunctionCavernous nerve injuryErectile dysfunctionCN injuryIntravenous infusionVehicle groupMSC groupIntracavernous pressureMesenchymal stem cellsNerve injuryBone marrowNeurotrophic factor expression levelsBrain-derived neurotrophic factorMale Sprague-Dawley ratsFluorogold-positive neuronsSmooth muscle atrophyPotential therapeutic effectsStem cellsSprague-Dawley ratsExpression levelsReal-time polymerase chain reactionBone marrow-derived mesenchymal stem cells
2015
Intravenous Preload of Mesenchymal Stem Cells Rescues Erectile Function in a Rat Model of Cavernous Nerve Injury
Takayanagi A, Sasaki M, Kataoka-Sasaki Y, Kobayashi K, Matsuda Y, Oka S, Masumori N, Kocsis JD, Honmou O. Intravenous Preload of Mesenchymal Stem Cells Rescues Erectile Function in a Rat Model of Cavernous Nerve Injury. The Journal Of Sexual Medicine 2015, 12: 1713-1721. PMID: 26211660, DOI: 10.1111/jsm.12957.Peer-Reviewed Original ResearchConceptsMajor pelvic ganglionGlia cell-derived neurotrophic factorArterial pressureCN injuryErectile dysfunctionIntracavernous pressureMesenchymal stem cellsMSC groupErectile functionDMEM groupNeurotrophic factorCavernous nerve injury modelMale Sprague-Dawley ratsNerve injury modelPotential preventive effectMale SD ratsImportant neurotrophic factorSprague-Dawley ratsReal-time quantitative real-time polymerase chain reactionExpression levelsReal-time polymerase chain reactionQuantitative real-time polymerase chain reactionMRNA expression levelsPelvic gangliaPolymerase chain reaction