2024
A surgical protocol for establishing spinal cord ischemia with extended lifespan and low complication rates in rats
Yasuda N, Sasaki M, Kocsis J, Kawaharada N, Honmou O. A surgical protocol for establishing spinal cord ischemia with extended lifespan and low complication rates in rats. World Neurosurgery 2024, 188: e349-e356. PMID: 38789035, DOI: 10.1016/j.wneu.2024.05.114.Peer-Reviewed Original ResearchIschemic spinal cord injurySpinal cord ischemiaCord ischemiaComplication rateRat modelTherapeutic strategiesEvaluate new therapeutic strategiesFunctional recoveryMale Sprague-Dawley ratsLow complication rateSprague-Dawley ratsSpecialized surgical equipmentExperimental animal modelsImprove functional recoveryPromote functional recoverySpinal cord injuryCross-clampingSevere neurological disordersAzygos veinSurgical protocolDescending aortaBulldog clampsLumbar levelsSpinal cordIschemic lesions
2022
Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury
Hirota R, Sasaki M, Kataoka-Sasaki Y, Oshigiri T, Kurihara K, Fukushi R, Oka S, Ukai R, Yoshimoto M, Kocsis JD, Yamashita T, Honmou O. Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury. Journal Of Neurotrauma 2022, 39: 1665-1677. PMID: 35611987, PMCID: PMC9734021, DOI: 10.1089/neu.2022.0106.Peer-Reviewed Original ResearchConceptsSpinal cord injuryCorticospinal tractMesenchymal stem cellsCord injurySpinal cordSpontaneous recoveryInfused mesenchymal stem cellsLimited spontaneous recoveryDorsal corticospinal tractLateral corticospinal tractStem cellsCST pathwayCST projectionsSCI inductionMSC infusionAxonal sproutingFunctional recoveryLateral funiculusIntravenous infusionAxonal tracerLesion coreMotor pathwaysFunctional improvementCircuit reorganizationMajor projections
2021
Focal brainstem infarction in the adult rat
Namioka A, Namioka T, Sasaki M, Kocsis JD, Honmou O. Focal brainstem infarction in the adult rat. Lab Animal 2021, 50: 97-107. PMID: 33564191, DOI: 10.1038/s41684-021-00722-1.Peer-Reviewed Original ResearchConceptsBrainstem ischemiaNew therapeutic approachesIschemic lesionsFunctional recoveryTherapeutic approachesAnimal modelsFemale Sprague-Dawley ratsBrainstem ischemic lesionsLower basilar arteryArtery occlusion modelIschemic lesion volumeType of strokeReliable animal modelSprague-Dawley ratsBrainstem infarctionExtended observation periodNeurological deficitsBasilar arteryLesion volumeOcclusion modelAdult ratsIschemiaPresent symptomsSurvival rateHigher survival rate
2019
Intravenous infusion of mesenchymal stem cells improves impaired cognitive function in a cerebral small vessel disease model
Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Suzuki J, Sasaki Y, Nagahama H, Hashi K, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells improves impaired cognitive function in a cerebral small vessel disease model. Neuroscience 2019, 408: 361-377. PMID: 30999031, DOI: 10.1016/j.neuroscience.2019.04.018.Peer-Reviewed Original ResearchConceptsCerebral small vessel diseaseBlood-brain barrierProgressive brain atrophyVascular dementiaMesenchymal stem cellsAlzheimer's diseaseCognitive functionBrain atrophyIntravenous infusionEffects of MSCsInfused mesenchymal stem cellsSmall vessel diseaseEssential pathological featuresAccumulation of AβSpinal cord injuryBBB transport systemsStem cellsBBB functionAβ accumulationHypertensive ratsNeuronal damageFunctional recoveryVessel diseaseCord injuryPathological features
2018
Intravenous infusion of mesenchymal stem cells promotes functional recovery in a rat model of chronic cerebral infarction.
Namioka T, Namioka A, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells promotes functional recovery in a rat model of chronic cerebral infarction. Journal Of Neurosurgery 2018, 131: 1289-1296. PMID: 30485210, DOI: 10.3171/2018.5.jns18140.Peer-Reviewed Original ResearchMiddle cerebral artery occlusionCerebral infarctionIntravenous infusionMesenchymal stem cellsChronic phaseRat modelBone marrowBlood-brain barrier integrityCerebral artery occlusionVehicle-treated groupChronic cerebral infarctionPhase of strokeStem cellsAdult bone marrowArtery occlusionBBB leakageMotor recoveryFunctional recoveryIschemic volumeFunctional outcomeSystemic infusionBrain parenchymaFunctional improvementSystemic injectionClinical studiesIntravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat.
Namioka A, Namioka T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat. Journal Of Neurosurgery 2018, 131: 1308-1316. PMID: 30485204, DOI: 10.3171/2018.4.jns173121.Peer-Reviewed Original ResearchBasilar artery occlusionIschemic lesion volumeInfused mesenchymal stem cellsIntravenous infusionMesenchymal stem cellsLesion volumeBrainstem infarctionMSC infusionFunctional recoveryIntravenous MSC infusionIschemic brainstem lesionsPosterior circulation strokeAcute stroke therapyArtery occlusion modelGroup 28 daysNylon monofilament sutureReliable animal modelMultiple behavioral testsStem cellsProximal BAArtery occlusionBrainstem lesionsPosterior circulationStroke therapyFunctional outcomeIntravenous Infusion of Mesenchymal Stem Cells Alters Motor Cortex Gene Expression in a Rat Model of Acute Spinal Cord Injury
Oshigiri T, Sasaki T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Oka S, Morita T, Hirota R, Yoshimoto M, Yamashita T, Hashimoto-Torii K, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Alters Motor Cortex Gene Expression in a Rat Model of Acute Spinal Cord Injury. Journal Of Neurotrauma 2018, 36: 411-420. PMID: 29901416, PMCID: PMC6352512, DOI: 10.1089/neu.2018.5793.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfused mesenchymal stem cellsMesenchymal stem cellsCord injuryIntravenous infusionFunctional improvementAcute spinal cord injuryBlood-spinal cord barrierGene expression signaturesAxonal sproutingFunctional recoveryMotor cortexSystemic infusionVehicle infusionSpinal cordRat modelTherapeutic mechanismImproved functionInfusionTranscription-polymerase chain reaction dataPearson correlation analysisBehavioral functionsExpression signaturesGenome-wide RNA profilingRecent evidenceFunctional recovery after the systemic administration of mesenchymal stem cells in a rat model of neonatal hypoxia-ischemia.
Sakai T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Fukumura S, Kobayashi M, Tsutsumi H, Kocsis JD, Honmou O. Functional recovery after the systemic administration of mesenchymal stem cells in a rat model of neonatal hypoxia-ischemia. Journal Of Neurosurgery Pediatrics 2018, 22: 513-522. PMID: 30074448, DOI: 10.3171/2018.5.peds1845.Peer-Reviewed Original ResearchConceptsNeonatal hypoxia-ischemiaMesenchymal stem cellsHypoxia-ischemiaRat modelHypoxic-ischemic encephalopathyStem cellsCerebral palsyFunctional recoveryFunctional outcomeSystemic administrationIntravenous administrationAnimal studiesBrain volumePotential treatmentAdministrationPalsyCellsPatientsEncephalopathySynaptogenesisPreservation of interhemispheric cortical connections through corpus callosum following intravenous infusion of mesenchymal stem cells in a rat model of cerebral infarction
Nagahama H, Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Namioka T, Namioka A, Oka S, Onodera R, Suzuki J, Sasaki Y, Kocsis JD, Honmou O. Preservation of interhemispheric cortical connections through corpus callosum following intravenous infusion of mesenchymal stem cells in a rat model of cerebral infarction. Brain Research 2018, 1695: 37-44. PMID: 29802840, DOI: 10.1016/j.brainres.2018.05.033.Peer-Reviewed Original ResearchConceptsInterhemispheric cortical connectionsCorpus callosumIntravenous infusionMesenchymal stem cellsDiffusion tensor imagingMotor cortexFunctional improvementCortical connectionsAdeno-associated virusRat middle cerebral artery occlusion stroke modelMiddle cerebral artery occlusion stroke modelContralateral motor cortexBlood-brain barrierMotor cortex connectivityVehicle-infused groupMRI diffusion tensor imagingPotential therapeutic mechanismNeuroanatomical tracing techniquesStem cellsCerebral infarctionInflammatory infiltrationAnatomical restorationCerebral strokeFunctional recoveryNeurotrophic factor
2016
Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury
Morita T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Oka S, Oshigiri T, Takebayashi T, Yamashita T, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury. Neuroscience 2016, 335: 221-231. PMID: 27586052, DOI: 10.1016/j.neuroscience.2016.08.037.Peer-Reviewed Original ResearchConceptsSpinal cord injuryIntravenous infusionMesenchymal stem cellsCord injuryBone marrowBlood-spinal cord barrier integrityPhases of SCIChronic spinal cord injuryOpen-field locomotor functionContusive spinal cord injurySevere Contusive Spinal Cord InjuryVehicle-treated groupStem cellsAdult bone marrowBSCB leakageMSC infusionExtensive remyelinationMotor recoveryFunctional recoverySerotonergic fibersCorticospinal tractSystemic infusionFunctional improvementSpinal cordRat model
2014
Possible neural plasticity detected by fMRI associates with improved motor function following intravenous injection of mesenchymal stem cells in a rat stroke model
Nakazaki M, Suzuki J, Sasaki M, Oka S, Sasaki Y, Honmou O. Possible neural plasticity detected by fMRI associates with improved motor function following intravenous injection of mesenchymal stem cells in a rat stroke model. Cerebral Blood Flow And Metabolism (Japanese Journal Of Cerebral Blood Flow And Metabolism) 2014, 25: 67. DOI: 10.16977/cbfm.25.2_67.Peer-Reviewed Original ResearchFunctional recoveryMesenchymal stem cellsLesion volumeSensorimotor cortexMotor functionInfused groupIschemic stroke model ratsStroke model ratsGreater functional recoveryImproved motor functionImproved functional outcomesAutologous mesenchymal stem cellsRat stroke modelRandomized-clinical trialFMRI patternsStem cellsCerebral infarctionStroke patientsFunctional outcomeIntravenous transplantationModel ratsMSCs groupFunctional deficitsSomatosensory cortexClinical trials
2012
Bilateral cortical hyperactivity detected by fMRI associates with improved motor function following intravenous infusion of mesenchymal stem cells in a rat stroke model
Suzuki J, Sasaki M, Harada K, Bando M, Kataoka Y, Onodera R, Mikami T, Wanibuchi M, Mikuni N, Kocsis JD, Honmou O. Bilateral cortical hyperactivity detected by fMRI associates with improved motor function following intravenous infusion of mesenchymal stem cells in a rat stroke model. Brain Research 2012, 1497: 15-22. PMID: 23274536, DOI: 10.1016/j.brainres.2012.12.028.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain InfarctionCerebral CortexDisease Models, AnimalExercise TestImage Processing, Computer-AssistedInfarction, Middle Cerebral ArteryMagnetic Resonance ImagingMesenchymal Stem Cell TransplantationMesenchymal Stem CellsMovement DisordersOxygenRatsRats, Sprague-DawleyStatistics, NonparametricTime FactorsConceptsMSC groupMesenchymal stem cellsLesion volumeFunctional MRISensorimotor cortexMotor functionElectrical stimulationRat cerebral infarction modelGreater functional recoveryCerebral infarction modelImproved motor functionImproved functional outcomesRat stroke modelHigh-intensity signalStem cellsFunctional recoveryBilateral signalsCortical hyperactivityFunctional outcomeIntravenous infusionIntravenous transplantationFunctional deficitsSomatosensory cortexInfused groupStroke model
2009
BDNF-Hypersecreting Human Mesenchymal Stem Cells Promote Functional Recovery, Axonal Sprouting, and Protection of Corticospinal Neurons after Spinal Cord Injury
Sasaki M, Radtke C, Tan AM, Zhao P, Hamada H, Houkin K, Honmou O, Kocsis JD. BDNF-Hypersecreting Human Mesenchymal Stem Cells Promote Functional Recovery, Axonal Sprouting, and Protection of Corticospinal Neurons after Spinal Cord Injury. Journal Of Neuroscience 2009, 29: 14932-14941. PMID: 19940189, PMCID: PMC2825276, DOI: 10.1523/jneurosci.2769-09.2009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain-Derived Neurotrophic FactorCells, CulturedCytoprotectionDisease Models, AnimalFemaleGene ExpressionGenetic VectorsGrowth ConesHumansMesenchymal Stem Cell TransplantationNerve RegenerationNeuronal PlasticityPyramidal TractsRatsRats, Sprague-DawleyRecovery of FunctionSpinal Cord InjuriesTransfectionTransplantation, HeterologousTreatment OutcomeConceptsSpinal cord injuryMesenchymal stem cellsCord injuryFunctional outcomeBone marrowAcute spinal cord injuryBrain-derived neurotrophic factorCorticospinal tract neuronsNumber of FGImproved functional outcomesPrimary motor cortexSpinal gray matterPotential therapeutic effectsStem cellsM1 cortexTransection lesionCorticospinal neuronsTract neuronsAxonal sproutingFunctional recoveryVentral hornNeuronal densitySerotonergic fibersLesion cavityMotor cortex
2005
28 Transplantation of Peripheral-Myelin-Forming Cells to Repair Demyelinated Axons
Kocsis J, Sasaki M. 28 Transplantation of Peripheral-Myelin-Forming Cells to Repair Demyelinated Axons. 2005, 421-433. DOI: 10.1016/b978-012738761-1/50029-8.Peer-Reviewed Original ResearchTransplantation of SCsTransplantation of OECsSpinal cord injuryCentral nervous systemSchwann cellsAxonal regenerationBone marrow cellsFunctional recoveryMultiple sclerosisCell transplantationCord injurySpinal cordHind limb locomotor functionContusive spinal cord injuryBone marrow cell transplantationMarrow cellsSpinal cord injury modelMarrow cell transplantationCerebral ischemia modelSpinal cord resultsGlobal neuroprotectionDemyelination modelCord resultsFunctional outcomeDemyelinated axons