2021
Intravenous Infusion of Mesenchymal Stem Cells Enhances Therapeutic Efficacy of Reperfusion Therapy in Cerebral Ischemia
Kiyose R, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Magota H, Oka S, Ukai R, Takemura M, Yokoyama T, Kocsis JD, Honmou O. Intravenous Infusion of Mesenchymal Stem Cells Enhances Therapeutic Efficacy of Reperfusion Therapy in Cerebral Ischemia. World Neurosurgery 2021, 149: e160-e169. PMID: 33618048, DOI: 10.1016/j.wneu.2021.02.056.Peer-Reviewed Original ResearchConceptsRegional cerebral blood flowInfused mesenchymal stem cellsReperfusion therapyPermanent MCAOIntravenous infusionMesenchymal stem cellsTherapeutic efficacyTransient middle cerebral artery occlusion modelMiddle cerebral artery occlusion modelArterial spin labeling magnetic resonance imagingArtery occlusion modelStandard therapeutic strategyTransient MCAO modelCerebral blood flowBehavioral functionsNon-favorable outcomeRat model systemStem cellsMCAO groupStroke inductionAcute strokeCerebral ischemiaNeurologic functionInfusion groupMCAO modelFocal brainstem infarction in the adult rat
Namioka A, Namioka T, Sasaki M, Kocsis JD, Honmou O. Focal brainstem infarction in the adult rat. Lab Animal 2021, 50: 97-107. PMID: 33564191, DOI: 10.1038/s41684-021-00722-1.Peer-Reviewed Original ResearchConceptsBrainstem ischemiaNew therapeutic approachesIschemic lesionsFunctional recoveryTherapeutic approachesAnimal modelsFemale Sprague-Dawley ratsBrainstem ischemic lesionsLower basilar arteryArtery occlusion modelIschemic lesion volumeType of strokeReliable animal modelSprague-Dawley ratsBrainstem infarctionExtended observation periodNeurological deficitsBasilar arteryLesion volumeOcclusion modelAdult ratsIschemiaPresent symptomsSurvival rateHigher survival rate
2018
Intravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat.
Namioka A, Namioka T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat. Journal Of Neurosurgery 2018, 131: 1308-1316. PMID: 30485204, DOI: 10.3171/2018.4.jns173121.Peer-Reviewed Original ResearchBasilar artery occlusionIschemic lesion volumeInfused mesenchymal stem cellsIntravenous infusionMesenchymal stem cellsLesion volumeBrainstem infarctionMSC infusionFunctional recoveryIntravenous MSC infusionIschemic brainstem lesionsPosterior circulation strokeAcute stroke therapyArtery occlusion modelGroup 28 daysNylon monofilament sutureReliable animal modelMultiple behavioral testsStem cellsProximal BAArtery occlusionBrainstem lesionsPosterior circulationStroke therapyFunctional outcome
2017
Elevated brain derived neurotrophic factor levels in plasma reflect in vivo functional viability of infused mesenchymal stem cells for stroke in rats.
Nakamura H, Sasaki Y, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Namioka T, Namioka A, Onodera R, Suzuki J, Nagahama H, Mikami T, Wanibuchi M, Kocsis JD, Honmou O. Elevated brain derived neurotrophic factor levels in plasma reflect in vivo functional viability of infused mesenchymal stem cells for stroke in rats. Journal Of Neurosurgical Sciences 2017, 63: 42-49. PMID: 28181779, DOI: 10.23736/s0390-5616.17.03989-3.Peer-Reviewed Original ResearchConceptsInfused mesenchymal stem cellsMiddle cerebral artery occlusionVivo functional viabilityBDNF levelsMSC infusionMesenchymal stem cellsMCAO inductionPlasma BDNFNeurotrophic factorLesion volumeMSC groupMiddle cerebral artery occlusion modelPermanent middle cerebral artery occlusionCerebral infarction rat modelBrain BDNF levelsElicit functional recoveryIntravenous MSC infusionLimb placement testNeurotrophic factor levelsPlasma BDNF levelsCerebral artery occlusionArtery occlusion modelRat stroke modelFunctional viabilityImmunosorbent assay analyses
2011
Development of a Middle Cerebral Artery Occlusion Model in the Nonhuman Primate and a Safety Study of I.V. Infusion of Human Mesenchymal Stem Cells
Sasaki M, Honmou O, Radtke C, Kocsis JD. Development of a Middle Cerebral Artery Occlusion Model in the Nonhuman Primate and a Safety Study of I.V. Infusion of Human Mesenchymal Stem Cells. PLOS ONE 2011, 6: e26577. PMID: 22039510, PMCID: PMC3200343, DOI: 10.1371/journal.pone.0026577.Peer-Reviewed Original ResearchConceptsMiddle cerebral arteryMagnetic resonance imagingM1 occlusionCollateral circulationSevere groupVenous bloodOcclusion modelMesenchymal stem cellsMiddle cerebral artery occlusion modelM1 branchNHP stroke modelsExtensive collateral circulationArtery occlusion modelPost-operative courseMCA territory infarctionGreater functional improvementLeft sylvian fissureNonhuman primate modelFronto-temporal craniotomyExperimental stroke researchAfrican green monkeysStem cellsHMSC infusionTerritory infarctionNeurological examination
2009
A Rat Middle Cerebral Artery Occlusion Model and Intravenous Cellular Delivery
Sasaki M, Honmou O, Kocsis JD. A Rat Middle Cerebral Artery Occlusion Model and Intravenous Cellular Delivery. Methods In Molecular Biology 2009, 549: 187-195. PMID: 19378204, DOI: 10.1007/978-1-60327-931-4_13.Peer-Reviewed Original ResearchConceptsMiddle cerebral artery occlusion modelArtery occlusion modelCerebral ischemiaIntraluminal sutureOcclusion modelRat middle cerebral artery occlusion modelInternal carotid arteryInterventional therapeutic approachesMagnetic resonance imagingUseful experimental modelNormal pathophysiologyInfarction volumeStroke therapyPermanent occlusionCarotid arteryReversible occlusionTherapeutic approachesIntravenous deliveryResonance imagingSuture insertionExperimental modelIschemiaAnimal weightPathophysiologySuture